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Citation:

Establishment of Optimum ISSR2PCR Reaction Systemin Tung O il Tree(Ve rn ic ia fordii)

  • Received Date: 2007-08-06
  • To app ly ISSR to genetic breeding of tung oil tree (Vernicia fordii) , both the single factor test and theorthogonal diagram were used to op timize the ISSR amp lification system of tung in four factors ( Taq DNApolymerase,Mg2+ , dNTP, p rimer) at three levels respectively. There were some differences between the results ofthe two methods. Through comp rehensive analysis, a suitable ISSR-PCR reaction system was established: 20 μLreaction system containing 1. 0 U Taq DNA polymerase, 2. 0 mmol·L-1 Mg2+ , 0. 20 mmol·L-1 dNTP, 0. 4μmol·L-1 p rimer, 1 ×PCR buffer, 40 ng temp late.
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Establishment of Optimum ISSR2PCR Reaction Systemin Tung O il Tree(Ve rn ic ia fordii)

  • 1. College of Life Science, Anhui Normal University, Wuhu 241000, Anhui, China
  • 2. Research Institute of Subtrop ical Forestry, CAF, Fuyang 311400, Zhejiang, China

Abstract: To app ly ISSR to genetic breeding of tung oil tree (Vernicia fordii) , both the single factor test and theorthogonal diagram were used to op timize the ISSR amp lification system of tung in four factors ( Taq DNApolymerase,Mg2+ , dNTP, p rimer) at three levels respectively. There were some differences between the results ofthe two methods. Through comp rehensive analysis, a suitable ISSR-PCR reaction system was established: 20 μLreaction system containing 1. 0 U Taq DNA polymerase, 2. 0 mmol·L-1 Mg2+ , 0. 20 mmol·L-1 dNTP, 0. 4μmol·L-1 p rimer, 1 ×PCR buffer, 40 ng temp late.

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