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Citation:

Study on the Sa lt Tolerance of Genetica llyMod if ied Tr iplo idChineseWhite Poplar

  • Received Date: 2007-10-10
  • The salt tolerance of tissue cultured p lantlet differentiation ofmodified trip loid Chinese white pop larwhich PLD /AtNHXI gene of saline resistance were transformed into were tested within 4 - 11 g·kg-1 salt concentration of 8 levels.Tissue cultured p lantlets rooting were tested within 2 - 10 g·kg-1 salt concentration of 9 levels. One year pot seedlingswere tested salt tolerance within 3 - 12 g · kg-1 salt concentration of 10 levels. The results showed that when saltconcentration reached 8 g · kg-1 , the genetically modified trip loid Chinese white pop lar began to have effects ondifferential growth, leaves color of tissue cultured p lantlets, number of differentiation buds and length of new growth, anddifferentiation of bottle seedlingswas restrained, gotwithered or dead with increasing of salt concentration, and that leavescolor had little effect, but rooting and new growth began to be restrained and rootage obviously reduced when tissuecultured p lantlets grew in rooting culture medium of 7 g·kg-1 salt concentration for 20 days, and that when pot seedlingsgrew for 32 days in the soil of 7 g·kg-1 salt concentration, they began to appear salt injury.
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Study on the Sa lt Tolerance of Genetica llyMod if ied Tr iplo idChineseWhite Poplar

  • 1. 11Taishan Institute of Forestry Science, Tai’an 271000, Shandong, China
  • 2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences,Beijing 100101, China
  • 3. Qingdao University of Science and Technology, Qingdao 266042, Shandong, China

Abstract: The salt tolerance of tissue cultured p lantlet differentiation ofmodified trip loid Chinese white pop larwhich PLD /AtNHXI gene of saline resistance were transformed into were tested within 4 - 11 g·kg-1 salt concentration of 8 levels.Tissue cultured p lantlets rooting were tested within 2 - 10 g·kg-1 salt concentration of 9 levels. One year pot seedlingswere tested salt tolerance within 3 - 12 g · kg-1 salt concentration of 10 levels. The results showed that when saltconcentration reached 8 g · kg-1 , the genetically modified trip loid Chinese white pop lar began to have effects ondifferential growth, leaves color of tissue cultured p lantlets, number of differentiation buds and length of new growth, anddifferentiation of bottle seedlingswas restrained, gotwithered or dead with increasing of salt concentration, and that leavescolor had little effect, but rooting and new growth began to be restrained and rootage obviously reduced when tissuecultured p lantlets grew in rooting culture medium of 7 g·kg-1 salt concentration for 20 days, and that when pot seedlingsgrew for 32 days in the soil of 7 g·kg-1 salt concentration, they began to appear salt injury.

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