Phylogenetic Rela tionsh ip Ana lysis among 13 Spec ies of P rim u la L.in South-west Sichuan Using RAPD Marker
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1.
Research Institute of Forestry, CAF
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2.
Key Laboratory of Tree Breeding Cultivation, State Forestry Administration,Beijing 100091, China
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Received Date:
2007-12-04
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Abstract
Using RAPD, the phylogenetic relationship of Prim ula L. from Longchi, Kangding, Mugecuo and L itangpopulationswas studied. The results indicated that 250 DNA fragments, which ranged from 130 bp to 1 800 bp,were amp lified based on 28 RAPD p rimers, 209 polymorphic fragments (83.6% ) were detected, and the averagenumber of DNA fragments per p rimerwas 9. The mean of genetic distance was 0.660 2. The dendrogram generatedbyUPGMA clustered all the 85 materials into 4 group s. Excep t P. vialii, P. prattii, P. m onticola and P. ovalifolia,the other specieswere not separated each other by the cluster. The result showed that P. secund if lora belonged toSect. Proliferae was also sustained, and there were distinct difference between Sect. Petiolares and Sect. A leuritiawith other sections. The dendrogram indicated that Longchi population was separated for Kangding, Mugecho andLitang populations, but the latter three populations were not distinguished each other. This suggested the eco-geographic characteristcs significantly influenced the genetic diversity of Prim ula L.
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