In V itro Culture and Plant Regenera tion of B auh in ia ga lpin ii

LU Xiu-li; ZHANG Qing-fei; BIAN Li-ming; WANG Rui; XU Shan-feng; GUI Ren-yi

Winter lignified stems of B auhinia galpinii were used as exp lants for tissue culture, and the effects ofdifferent hormones on inductivity rate and rooting rate were investigated by supp lementing different concentrations.The results indicated that budswere induced from a 2 cm high young p lantlet cultured inMSmedium supp lementedwith 1. 0 mg·L-1 62BA and 0. 1 mg·L-1 NAA for 20 d, and the induction rate was 100%. The mean amount ofbuds was 8. 7. The medium ofMS + 0. 4mg·L-1 NAA was used for rooting and the rooting rate was 85%. Theexperiment strategy to establish tissue culture system from lignified stem was feasible, which could be used for coldacclimation and cold resistantmutant selection.

1. Shanghai Landscape Gardening Research Institute, Shanghai　200232, China

2. Key Laboratory of Forest Genetics and Gene Engineering,Nanjing Forestry University, Nanjing　210037 , J iangsu, China

3. Key Laboratory of Forest Genetics and Gene Engineering,Nanjing Forestry University, Nanjing　210037 , J iangsu, China

4. Shanghai Forestry Station, Shanghai　200072, China

Received Date: 2007-10-08

Abstract: Winter lignified stems of B auhinia galpinii were used as exp lants for tissue culture, and the effects ofdifferent hormones on inductivity rate and rooting rate were investigated by supp lementing different concentrations.The results indicated that budswere induced from a 2 cm high young p lantlet cultured inMSmedium supp lementedwith 1. 0 mg·L-1 62BA and 0. 1 mg·L-1 NAA for 20 d, and the induction rate was 100%. The mean amount ofbuds was 8. 7. The medium ofMS + 0. 4mg·L-1 NAA was used for rooting and the rooting rate was 85%. Theexperiment strategy to establish tissue culture system from lignified stem was feasible, which could be used for coldacclimation and cold resistantmutant selection.

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Reference (19)

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In V itro Culture and Plant Regenera tion of B auh in ia ga lpin ii

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通讯作者: 陈斌, bchen63@163.com

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