Analysis of Genetic Diversity and Differentiation for Provenances of Manglietia yuyuanensis

LI Yin-gang; ZHOU Zhi-chun; FAN Hui-hua; HONG Chang-sheng; JIN Guo-qing

Volume 21 Issue 4

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Article Navigation > Forest Research > 2008 > 21(4): 582-586

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Analysis of Genetic Diversity and Differentiation for Provenances of Manglietia yuyuanensis

1.
Research Institute of Subtropical Forestry,CAF,Fuyang 311400,Zhejiang,China
2.
Extending Center for Forestry S & T of Jian'ou City,Fujian Province,Jian'ou 353100,Fujian,China
3.
Forestry Bureau of Longquan City,Zhejiang Province,Longquan 323700,Zhejiang,China

Abstract

Genetic diversity and genetic differentiation of 11 Manglietia yuyuanensis provenances from main distribution area were analyzed using ISSR molecular markers.A total of 108 amplified loci were detected by 9 primers,of which the proportion of polymorphic loci was 86.11%.A high degree of genetic diversity in M.yuyuanensis was detected,and the genetic diversity index in species level was 0.282 7,which was markedly higher than that of M.decidua and M.patungensis,the rare and endangered species belonging to the same genus with M.yuyuanensis.The result showed that there was significant difference in genetic diversity among provenances.Provenances from north and north-west Fujian province and the nearby area,including Shaxian,Jian'ou,Wuyishan of Fujian and Longquan of Zhejiang,had higher genetic diversity,which were the dominant regions to be protected and utilized. Small population effect brought by habitat fragmentation and distance isolation effect resulted in high genetic differentiation among provenances,which contributed to 25.89% of total genetic variation,and the differentiation within provenances contributed to 74.11% of total genetic variation.Results also showed that the genetic distance amog provenances was not significantly related to the geographical distance amog provenances.Based on the Nei's unbiased genetic distance,11 provenances might be obviously divided into 3 clusters.Shaxian and Jian'ou of Fujian with high genetic diversity and large nature population was clustered into one group.The genetic distance among Huangshan of Anhui,Longquan and Kaihua of Zhejiang,Wuyishan,Shaowu and Liancheng of Fujian,and Lichuan and Longnan of Jiangxi was small,then the above provenances were clustered.Quannan of Jiangxi,located at the south of distribution area,was an individual provenance.Based on the characteristic of its wider leaves,it was conferred that Quannan provenance was mistaken for M.fordiana Oliv.in the course of seed collection.
- Manglietia yuyuanensis,
- provenance,
- genetic diversity,
- genetic differentiation,
- ISSR

References

[1]	叶桂艳. 中国木兰科树种[M]. 北京:中国农业出版社, 1996
[2]	金国庆,周志春,胡红宝,等. 3种乡土阔叶树种容器育苗技术研究[J]. 林业科学研究, 2005, 17 (4) : 387 - 392
[3]	王忠平,陈水龙,赖培淼,等. 乳源木莲造林试验研究初报[J]. 福建林学院学报, 1996, 16 (4) : 315 - 318
[4]	周志春,李建民,陈炳星,等. 几种亚热带速生乡土阔叶树种的制浆特性评价[J]. 中国造纸, 2003, 22 (2) : 8 - 12
[5]	陈存及,曹永慧,董建文,等. 乳源木莲天然林优势种群结构与空间格局[J]. 福建林学院学报, 2001, 21 (3) : 207 - 211
[6]	曹永慧,陈存及,赖培淼,等. 乳源木莲天然林群落种间联结的研究[J]. 福建林学院学报, 2003, 23 (2) : 124 - 127
[7]	李因刚, 周志春,金国庆,等. 乳源木莲苗生长和形态的地理种源分化[J]. 林业科学研究, 2007, 20 (1) : 35 - 39
[8]	林新春,俞志雄,裘利洪,等. 濒危植物华木莲的遗传多样性研究[J]. 江西农业大学学报, 2003, 25 (6) : 805 - 810
[9]	廖文芳,夏念和,邓云飞,等. 华木莲的遗传多样性研究[J]. 云南植物研究, 2004, 26 (1) : 58 - 64
[10]	何敬胜,李作洲,黄宏文. 濒危物种巴东木莲的等位酶遗传多样性及其保护策略[J]. 生物多样性, 2005, 13 (1) : 27 - 35
[11]	Huang J C, Sun M. Genetic diversity and relationship s of sweetpotato and its wild relatives in Ipom oea series Batatas (Convolvulaceae) as revealed by inter-simp le sequence repeat ( ISSR) andrestriction analysis of chlorop lastDNA[J]. Theoretical and App liedGenetics, 2000, 100: 1050 - 1060
[12]	MartinsM, Tenreiro R, OliveiraM M. Genetic relatedness of Portuguese alm ond cultivars assessed by RAPD and ISSR markers[J].Plant Cell Reports, 2003, 22: 71 - 78
[13]	Panda S,Martin J, Aguinagalde I. Chlorop last and nuclear DNAstudies in a few members of the B rassica oleracea L. group usingPCR-RFLP and ISSR-PCR markers: a population genetic analysis[J]. Theoretical and App lied Genetics, 2003, 106: 1122 - 1128
[14]	Wolfe A D, Liston A. Contributions of PCR-based methods to p lantsystematics and evolutionary biology[M] / / SoltisD E, Soltis P S,Doyle J J. Molecular systematics of p lants II. DNA Sequencing.Norwell,M A: KluwerAcademic Publishers, 1998: 43 - 86
[15]	Harris J. RAPDs in systematic-a useful methodology? [M] / /Hollingsworth PM, Bateman RM, Gornall R J. Molecular systematics and p lant evolution. London: Taylor and Francis, 1999: 221- 228
[16]	Tsumura Y, Ohba K, Strauss S H. Diversity and inheritance of inter-simp le sequence repeat polymorphism in Douglas-fir ( Pseudotsuga m enziesii) and sugi (Cryptom eria japonica1) [J]. Theoreticaland App lied Genetics, 1994, 92: 40 - 45
[17]	Doyle J J, Doyle J L. A rap id DNA isolation p rocedure for smallquantities of fresh leaf tissue [J]. Phytochem Bull, 1987, 19: 11- 15
[18]	Williams J G K, Kubelik A R,Livak K J, et al. DNA polymorphismsamp lified by arbitrary p rimers are useful as geneticmarkers[J]. Nucleic Acids Research, 1990, 18 (22) : 6531 - 6535
[19]	钱　韦,葛　颂. 居群遗传结构中显性标记数据分析方法初探[J]. 遗传学报, 2000, 28 (3) : 244 - 255
[20]	Lynch M,Milligan B G. Analysis of population genetic structurewith RAPD markers[J]. Molecular Ecology, 1994, 3 (2) : 91 - 99

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Analysis of Genetic Diversity and Differentiation for Provenances of Manglietia yuyuanensis

1. Research Institute of Subtropical Forestry,CAF,Fuyang 311400,Zhejiang,China

2. Extending Center for Forestry S & T of Jian'ou City,Fujian Province,Jian'ou 353100,Fujian,China

3. Forestry Bureau of Longquan City,Zhejiang Province,Longquan 323700,Zhejiang,China

Abstract: Genetic diversity and genetic differentiation of 11 Manglietia yuyuanensis provenances from main distribution area were analyzed using ISSR molecular markers.A total of 108 amplified loci were detected by 9 primers,of which the proportion of polymorphic loci was 86.11%.A high degree of genetic diversity in M.yuyuanensis was detected,and the genetic diversity index in species level was 0.282 7,which was markedly higher than that of M.decidua and M.patungensis,the rare and endangered species belonging to the same genus with M.yuyuanensis.The result showed that there was significant difference in genetic diversity among provenances.Provenances from north and north-west Fujian province and the nearby area,including Shaxian,Jian'ou,Wuyishan of Fujian and Longquan of Zhejiang,had higher genetic diversity,which were the dominant regions to be protected and utilized. Small population effect brought by habitat fragmentation and distance isolation effect resulted in high genetic differentiation among provenances,which contributed to 25.89% of total genetic variation,and the differentiation within provenances contributed to 74.11% of total genetic variation.Results also showed that the genetic distance amog provenances was not significantly related to the geographical distance amog provenances.Based on the Nei's unbiased genetic distance,11 provenances might be obviously divided into 3 clusters.Shaxian and Jian'ou of Fujian with high genetic diversity and large nature population was clustered into one group.The genetic distance among Huangshan of Anhui,Longquan and Kaihua of Zhejiang,Wuyishan,Shaowu and Liancheng of Fujian,and Lichuan and Longnan of Jiangxi was small,then the above provenances were clustered.Quannan of Jiangxi,located at the south of distribution area,was an individual provenance.Based on the characteristic of its wider leaves,it was conferred that Quannan provenance was mistaken for M.fordiana Oliv.in the course of seed collection.

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Reference (20)

[1]	叶桂艳. 中国木兰科树种[M]. 北京:中国农业出版社, 1996
[2]	金国庆,周志春,胡红宝,等. 3种乡土阔叶树种容器育苗技术研究[J]. 林业科学研究, 2005, 17 (4) : 387 - 392
[3]	王忠平,陈水龙,赖培淼,等. 乳源木莲造林试验研究初报[J]. 福建林学院学报, 1996, 16 (4) : 315 - 318
[4]	周志春,李建民,陈炳星,等. 几种亚热带速生乡土阔叶树种的制浆特性评价[J]. 中国造纸, 2003, 22 (2) : 8 - 12
[5]	陈存及,曹永慧,董建文,等. 乳源木莲天然林优势种群结构与空间格局[J]. 福建林学院学报, 2001, 21 (3) : 207 - 211
[6]	曹永慧,陈存及,赖培淼,等. 乳源木莲天然林群落种间联结的研究[J]. 福建林学院学报, 2003, 23 (2) : 124 - 127
[7]	李因刚, 周志春,金国庆,等. 乳源木莲苗生长和形态的地理种源分化[J]. 林业科学研究, 2007, 20 (1) : 35 - 39
[8]	林新春,俞志雄,裘利洪,等. 濒危植物华木莲的遗传多样性研究[J]. 江西农业大学学报, 2003, 25 (6) : 805 - 810
[9]	廖文芳,夏念和,邓云飞,等. 华木莲的遗传多样性研究[J]. 云南植物研究, 2004, 26 (1) : 58 - 64
[10]	何敬胜,李作洲,黄宏文. 濒危物种巴东木莲的等位酶遗传多样性及其保护策略[J]. 生物多样性, 2005, 13 (1) : 27 - 35
[11]	Huang J C, Sun M. Genetic diversity and relationship s of sweetpotato and its wild relatives in Ipom oea series Batatas (Convolvulaceae) as revealed by inter-simp le sequence repeat ( ISSR) andrestriction analysis of chlorop lastDNA[J]. Theoretical and App liedGenetics, 2000, 100: 1050 - 1060
[12]	MartinsM, Tenreiro R, OliveiraM M. Genetic relatedness of Portuguese alm ond cultivars assessed by RAPD and ISSR markers[J].Plant Cell Reports, 2003, 22: 71 - 78
[13]	Panda S,Martin J, Aguinagalde I. Chlorop last and nuclear DNAstudies in a few members of the B rassica oleracea L. group usingPCR-RFLP and ISSR-PCR markers: a population genetic analysis[J]. Theoretical and App lied Genetics, 2003, 106: 1122 - 1128
[14]	Wolfe A D, Liston A. Contributions of PCR-based methods to p lantsystematics and evolutionary biology[M] / / SoltisD E, Soltis P S,Doyle J J. Molecular systematics of p lants II. DNA Sequencing.Norwell,M A: KluwerAcademic Publishers, 1998: 43 - 86
[15]	Harris J. RAPDs in systematic-a useful methodology? [M] / /Hollingsworth PM, Bateman RM, Gornall R J. Molecular systematics and p lant evolution. London: Taylor and Francis, 1999: 221- 228
[16]	Tsumura Y, Ohba K, Strauss S H. Diversity and inheritance of inter-simp le sequence repeat polymorphism in Douglas-fir ( Pseudotsuga m enziesii) and sugi (Cryptom eria japonica1) [J]. Theoreticaland App lied Genetics, 1994, 92: 40 - 45
[17]	Doyle J J, Doyle J L. A rap id DNA isolation p rocedure for smallquantities of fresh leaf tissue [J]. Phytochem Bull, 1987, 19: 11- 15
[18]	Williams J G K, Kubelik A R,Livak K J, et al. DNA polymorphismsamp lified by arbitrary p rimers are useful as geneticmarkers[J]. Nucleic Acids Research, 1990, 18 (22) : 6531 - 6535
[19]	钱　韦,葛　颂. 居群遗传结构中显性标记数据分析方法初探[J]. 遗传学报, 2000, 28 (3) : 244 - 255
[20]	Lynch M,Milligan B G. Analysis of population genetic structurewith RAPD markers[J]. Molecular Ecology, 1994, 3 (2) : 91 - 99

Analysis of Genetic Diversity and Differentiation for Provenances of Manglietia yuyuanensis

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