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Citation:

Analysis of Genetic Diversity in Lycoris radiata Using ISSRMarker

  • Received Date: 2007-12-03
  • The genetic diversity of 14 Lycoris radiata populations was analyzed by ISSR markers. The results ofPOPGEN32 analysis showed that at species level, the percentage of polymorphics was 92. 31% , Shannon’s index(H) was 0. 459 7, and Nei’s gene diversity ( I) was 0. 302 5, indicating a high level of genetic diversity. But atpopulation level, theywere 49. 65% , 0. 262 0 and 0. 176 3, respectively, suggesting a low level of genetic diversity.And the gene differentiation coefficient (Gst) and gene flow (Nm ) among the populations were 0. 503 5 and0. 698 3, respectively. The analysis ofmolecular variance (AMOVA) demonstrated that there was a relatively highlevel genetic variation (46. 12% ) among the populations, and there was relatively low genetic variation (53. 88% )within the populations. The high genetic differentiation among populations and the low genetic diversity withinpopulations could be attributed to the habitat fragmentation and the limited gene flow among populations.
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    [2] 杨志玲,谭梓峰. 红花石蒜资源的开发利用和繁育研究建议[J]. 经济林研究, 2003, 21 (4) : 97 - 99

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    [14] 崔海丽,夏春镗,赵则胜. 黑稻资源遗传多样性的SSR分析[J].中国农学通报, 2007, 23 (1) : 63 - 67

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Analysis of Genetic Diversity in Lycoris radiata Using ISSRMarker

  • 1. Research Institute of Subtrop ical Forestry, CAF, Fuyang 311400, Zhejiang, China
  • 2. Central South University of Forestry and Technology, Changsha 410004, Hu’nan , China

Abstract: The genetic diversity of 14 Lycoris radiata populations was analyzed by ISSR markers. The results ofPOPGEN32 analysis showed that at species level, the percentage of polymorphics was 92. 31% , Shannon’s index(H) was 0. 459 7, and Nei’s gene diversity ( I) was 0. 302 5, indicating a high level of genetic diversity. But atpopulation level, theywere 49. 65% , 0. 262 0 and 0. 176 3, respectively, suggesting a low level of genetic diversity.And the gene differentiation coefficient (Gst) and gene flow (Nm ) among the populations were 0. 503 5 and0. 698 3, respectively. The analysis ofmolecular variance (AMOVA) demonstrated that there was a relatively highlevel genetic variation (46. 12% ) among the populations, and there was relatively low genetic variation (53. 88% )within the populations. The high genetic differentiation among populations and the low genetic diversity withinpopulations could be attributed to the habitat fragmentation and the limited gene flow among populations.

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