The Extraction of Polysacchar ide from WhiteWax Sca le andAna lysis ofMonosacchar ide Compositions

HE Zhao; SUN Long; FENG Ying; CHENG Xiao-ming

WhiteWax Scale ( Ericerus pela Chavannes) polysaccharide was extracted with water, and p recip itated byalcohol. The contents of polysaccharide、p rotein and amino acidswere measured. The cap illary zone electrophoresis(CZE) method was developed to separate and identify the monosaccharide composition of white wax scalepolysaccharide. In thismethod, white wax scale polysaccharide was hydrolyzed into componentmonosaccharides andsubsequently derived with 1-phenyl-3-methyl-5-pyrazolone ( PMP) , the concentration and pH of bufferwere studiedand op timized. The results showed extracting ratio of polysaccharide was 2. 4% , content of polysaccharide was256 mg·g-1 content of p rotein was 242 mg·g-1 , the polysaccharide consisted of glucose, mannose, galactose withthe molar ratio of 1∶0. 85∶0. 93.

1. Research Institute of Resources Insects, CAF, Laboratory of Cultivation and Utilization of Resource Insects,State Forestry Administration, Kunming　650224, Yunnan, China

Received Date: 2008-06-04

Abstract: WhiteWax Scale ( Ericerus pela Chavannes) polysaccharide was extracted with water, and p recip itated byalcohol. The contents of polysaccharide、p rotein and amino acidswere measured. The cap illary zone electrophoresis(CZE) method was developed to separate and identify the monosaccharide composition of white wax scalepolysaccharide. In thismethod, white wax scale polysaccharide was hydrolyzed into componentmonosaccharides andsubsequently derived with 1-phenyl-3-methyl-5-pyrazolone ( PMP) , the concentration and pH of bufferwere studiedand op timized. The results showed extracting ratio of polysaccharide was 2. 4% , content of polysaccharide was256 mg·g-1 content of p rotein was 242 mg·g-1 , the polysaccharide consisted of glucose, mannose, galactose withthe molar ratio of 1∶0. 85∶0. 93.

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The Extraction of Polysacchar ide from WhiteWax Sca le andAna lysis ofMonosacchar ide Compositions

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References

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