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Citation:

Study on Molecular Identif ica tion and Genetic Rela tionships ofEctomycorrhiza l Stra ins of Su illus Gene

  • Received Date: 2007-04-10
  • The molecular identification and genetic diversity of 27 Su illus strains isolated from InnerMongolia werestudied based on the sequencing analysis of ITS, the internal transcribed spacer region of the ribosomal RNA genes,and ITS-RFLP for the first time. ITS sequences of 27 Suillus strainswere subjected to a GenBank search and SP1,SP2, SP3, SP4, SP5, SP6, SP7, SP8, SP9, SF1, SF2, SF3, P. Lac-1, P. Lac-2, 3′, 3″, 3# were belonged to Suillus lu teuswith 99% similarity to DQ440568. 1 in GenBank. These S uillus strains were classified into 3 genotypes. The greatgenetic distance of“zhang2”and 1″from others might be the results of the influence by its hosts and habitats.Although sequence analysis of ITS was accurate, the ITS-RFLP was simp le and p ractical and might be useful to thequick identification of ectomycorrhizal fungi. It was more effective and accurative for using these two techniquescombination.
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Study on Molecular Identif ica tion and Genetic Rela tionships ofEctomycorrhiza l Stra ins of Su illus Gene

  • 1. College of Forestry, InnerMongolia Agricultural University, Hohhot 010019, InnerMongolia, China
  • 2. Research Institute of Forestry, CAF, Beijing 100091, China

Abstract: The molecular identification and genetic diversity of 27 Su illus strains isolated from InnerMongolia werestudied based on the sequencing analysis of ITS, the internal transcribed spacer region of the ribosomal RNA genes,and ITS-RFLP for the first time. ITS sequences of 27 Suillus strainswere subjected to a GenBank search and SP1,SP2, SP3, SP4, SP5, SP6, SP7, SP8, SP9, SF1, SF2, SF3, P. Lac-1, P. Lac-2, 3′, 3″, 3# were belonged to Suillus lu teuswith 99% similarity to DQ440568. 1 in GenBank. These S uillus strains were classified into 3 genotypes. The greatgenetic distance of“zhang2”and 1″from others might be the results of the influence by its hosts and habitats.Although sequence analysis of ITS was accurate, the ITS-RFLP was simp le and p ractical and might be useful to thequick identification of ectomycorrhizal fungi. It was more effective and accurative for using these two techniquescombination.

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