Study on Culture Media for Micropropagation of Oplopanax elatus Nakai
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1.
Department of Biology, Tonghua Normal University, Tonghua 134002, Jilin, China
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Received Date:
2007-09-15
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Abstract
Taking the tender leaves ofOplopanax elatus as exp lants for the experiment, Uniform Design were used totest the most suitable media for callus induction, differentiation of shoot and rooting. The results showed thatN6 +6-BA 0. 5 mg·L-1 + 2, 4-D 0. 2 mg·L-1 +Vc 10 mg·L-1 was fitted for callus induction, the medium fordifferentiation of shootwas N6 + 6-BA 4. 5 mg·L-1 +NAA 0. 1 mg·L-1 +Vc 20 mg·L-1 , the medium forrooting was 1 /4MS + IBA 0. 1mg·L-1 +NAA 0. 1 mg·L-1. Tissue culture required different kinds of culturemedia in different phases.
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