Construction and Analysis of the Suppression SubtractiveHybridization L ibrary of Larix Cuttings during Rooting

FENG Jian; QI Li-wang; SUN Xiao-mei; HAN Su-ying; ZHANG Shou-gong

Taking the stem s and roots of tw o fu ll sib clones w ith significant d ifference in roo ting rate as testmaterials, tw o differential expression cDNA librar ies o f Larix cutting during root ing w ere established by PCR se lectTM cDNA subtract ion k i.t The resu lts of analysis of Rsa I d igest ion, ligat ion and subtraction eff iciency show edtha t the tw o libraries w ere established successfully. The posit ive clones w ere screened by PCR and dot blots. 521UniEST w ere obta ined by sequenc ing and b io informat ics ana lysis.

1. Group of Trees Cell Biology, Research Inst itu te of Forestry, CAF, Beijing 100091, Ch ina

2. Liaon ing A cad emy of Forestry,Shenyang 110032, L iaon ing, Ch ina

3. Research Inst itu te o fForest Eco logy, Environment and Protect ion, CAF, Beijing 100091, Ch ina

Received Date: 2008-03-07

Abstract: Taking the stem s and roots of tw o fu ll sib clones w ith significant d ifference in roo ting rate as testmaterials, tw o differential expression cDNA librar ies o f Larix cutting during root ing w ere established by PCR se lectTM cDNA subtract ion k i.t The resu lts of analysis of Rsa I d igest ion, ligat ion and subtraction eff iciency show edtha t the tw o libraries w ere established successfully. The posit ive clones w ere screened by PCR and dot blots. 521UniEST w ere obta ined by sequenc ing and b io informat ics ana lysis.

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Reference (13)

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Construction and Analysis of the Suppression SubtractiveHybridization L ibrary of Larix Cuttings during Rooting

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References

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