G iem sa C-band ing, F ISH and Karyotype Ana lysis ofPhyllosta chys pubescens var. heterocycla Chrom osom e

XU Chuan-mei; ZHENG Hua-wei; WANG Cong; TANG Ding-qin

Giemsa C-band ing and FISH analysis of the Ph. pubescens var. heterocycla chromosomes from root tipswere carried ou t in this research. The resu lts showed that the C-band ing karyotypewou ld be 2n= 48= 4CI+ 2CI+ T+ 2CT+ + 10CT+ + 4I+ 2I+ + 6T+ + 6C+ 8T + 2IT + 2I+ T. The specific C-band ing points on each chromosomecou ld be distinguished and the d ifferences ofC-band ing intensity a lso could be recognized. 45S rDNA was used asprobe for FISH analysis, and one couple of hybridization signals was d isp layed on the chromosome centrimere. Inthis research we have a conclusion that chromosomes ofPh. pubescens var. heterocycla can be easily distinguishedand identified by Giemsa C-band ing and FISH approaches.

1. Key Lab forModern Silvicultural Techno logy of Zh ejiang Province, Zhejiang ForestryUn ivers ity, Lin. An 311300, Zh ejiang, Ch ina

Received Date: 2008-12-03

Abstract: Giemsa C-band ing and FISH analysis of the Ph. pubescens var. heterocycla chromosomes from root tipswere carried ou t in this research. The resu lts showed that the C-band ing karyotypewou ld be 2n= 48= 4CI+ 2CI+ T+ 2CT+ + 10CT+ + 4I+ 2I+ + 6T+ + 6C+ 8T + 2IT + 2I+ T. The specific C-band ing points on each chromosomecou ld be distinguished and the d ifferences ofC-band ing intensity a lso could be recognized. 45S rDNA was used asprobe for FISH analysis, and one couple of hybridization signals was d isp layed on the chromosome centrimere. Inthis research we have a conclusion that chromosomes ofPh. pubescens var. heterocycla can be easily distinguishedand identified by Giemsa C-band ing and FISH approaches.

HTML

Reference (18)

[1]	H un ter AW S. A karyo systemat ic invest igat ion in th e G ram ineae[J]. Can ad ian Jou rnalResearch, 1934, 11(4): 213- 241
[2]	M cClu re F A. N ew genera and species ofBambu so ideae from eastern[J]. A sia L ingn an Scien ce Bu lletin, 1940, 9: 66- 67
[3]	李秀兰, 刘松, 宋文芹, 等. 40 种散生竹的染色体数目[J]. 植物分类学报, 1999, 37(6): 541- 544
[4]	李秀兰, 林汝顺, 冯学琳, 等. 中国部分丛生竹类染色体数目报道[J]. 植物分类学报, 2001, 39 (5): 433 - 442
[5]	陈瑞阳, 宋文芹, 李秀兰, 等. 中国主要经济植物基因组染色体图谱(第四册: 中国竹类染色体图谱) [M]. 北京: 科学出版社, 2003
[6]	G illB S. G iemsa band ing techn ique for cereal ch romosome [J]. CerealR esearch Commun ication, 1974, 2: 87- 94
[7]	G il lB S, Friebe B, EndoT R. Standard karyotype and nomen clatu resystem for descrip tion of ch romosome b ands and structu ral aberrat ion sin wh eat (Triticum aestivum L.) [J]. Genome, 1991, 34: 830- 839
[8]	李懋学, 陈瑞阳. 关于植物核型分析的标准化问题[J]. 武汉植物学研究, 1985, 3(4): 297- 302
[9]	刘光欣, 胡凤荣, 席梦利, 等. 岷江百合根尖染色体的C-分带和FISH 分析[J]. 分子植物育种, 2008, 6(1): 95- 99
[10]	陈佩度. 用分子原位杂交(GISH)鉴定小麦簇毛麦双二倍体、附加系、代换系和易位系[J]. 遗传学报, 1995b, 22 (5):380- 386
[11]	Jiang J M, Friebe B, G ill B S. Recent advances in a lien genetran sfer in wheat[J]. Euphytica, 1993, 73: 199- 212
[12]	Levan A, Fredga K, SandbergA A. Nomenclatu re for cen tromericposit ion on ch romosome[J]. H ered itas, 1964, 52: 201- 220
[13]	Stebb in sG L. Ch romosomal evolu tion in h igher p lants [M]. London: EdwardA rnold Ltd, 1971: 87- 89
[14]	A ranoH. Cytological stud ies in sub familyCarduoidae of Japan ù:Th e k aryotype analys is and phylogen ic cons ideration on Pertya andAinslia ea (2) [J]. BotMagTokyo, 1963, 76: 32- 39
[15]	刘大钧. 细胞遗传学[M]. 北京: 中国农业出版社, 1998: 10 - 11
[16]	陈瑞阳, 宋文芹, 李秀兰, 等. 中国主要经济植物基因组染色体图谱(第二册: 中国农作物及其野生近源植物染色体图谱)[M]. 北京: 科学出版社, 2003
[17]	樊龙江, 郭兴益, 马乃训. 竹类植物与水稻等其它禾本科作物的系统进化关系及基因序列组成的比较[J]. 林业科学研究,2006, 19 (2): 165 - 169
[18]	徐川梅, 卢江杰, 汤定钦. 利用FISH 技术研究45S rDNA 在部分竹种染色体上的分布[J]. 林业科学(待发表)

G iem sa C-band ing, F ISH and Karyotype Ana lysis ofPhyllosta chys pubescens var. heterocycla Chrom osom e

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views