SDS-PAGE Ana lysis on Prote in ofH yphan tria cunea Nuclear Polyhedrosis Virus

XUE Jian-jie; QU Liang-jian; WANG Yu-zhu; ZHANG Yong-an; TANG Ming; YANG Wei-yi

Hyphantria cuea nucleopolyhedrovirus (HcNPV) and several other nucleopolyhedrosis was extracted bydifferential centrifugation, and purified with sucrose gradient centrifugation method. Polyhedrin was gotten andanalysed by SDS - PAGE. It indicated thatmost of those p roteins had 32 KD p rotein band, and with 64 KD p rotein,and might be the main p rotein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virusp rotein in 18 KD department had a specific band.

1. College of Forestry,Northwest Sci-Tech University ofAgriculture and Forestry, Yangling　712100, Shaanxi, China

2. Research Institute of Forest Ecology, Environment and Protection, CAF, Key Laboratory of Forest Protection,State Forestry Administration,Beijing

Received Date: 2008-09-09

Abstract: Hyphantria cuea nucleopolyhedrovirus (HcNPV) and several other nucleopolyhedrosis was extracted bydifferential centrifugation, and purified with sucrose gradient centrifugation method. Polyhedrin was gotten andanalysed by SDS - PAGE. It indicated thatmost of those p roteins had 32 KD p rotein band, and with 64 KD p rotein,and might be the main p rotein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virusp rotein in 18 KD department had a specific band.

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Reference (13)

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SDS-PAGE Ana lysis on Prote in ofH yphan tria cunea Nuclear Polyhedrosis Virus

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