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Cloning and Sequence Analysis of CONSTANS Homologous Gene from Dendrocalamus xishuangbannaensis

  • CONSTANS (CO) is one of the key genes controlling flowering time of the flowering plants in Photoperiod Pathways. In current study, two primers, COS1 and COA1, based on the conserved region of Hd1 sequences from CO-like gene of rice (Oryza sativa), were employed to amplify a 1 520 bp fragment by polymerase chain reaction (PCR) with genomic DNA of Dendrocalamus xishuangbannaensis D. Z. Li & H. Q. Yang. The fragment was cloned into a pGEM-T vector and then sequenced. Result of the 1 520 bp fragment cloned into a pGEM-T vector produced a fragment of gene, i.e. DxCO1, with accession number GQ358925 in GenBank. The DNA sequence analysis indicated that DxCO1 included one intron of 590 bp and two exons encoding putative 310 amino acids. Using homology analyses of DNA sequence, DxCO1 was homologous to the other six CO-like genes from Gramineae plants with 81%-91% supports, respectively. Phylogenetic analysis, based on protein sequences of DxCO1 and the known CO-like genes of seed plant in GenBank, showed that DxCO1 and other five CO-like genes from Gramineae plants clustered into a monophyly with bootstrap value of 92%. Meanwhile, there was a zinc-finger B-box (Cx2Cx8Cx7Cx2Cx4Hx8H) domain near amino terminus and a CCT (CO,CO-like, TOC1) domain near carboxy terminus in putative amino acid sequence of DxCO1. These results suggested that DxCO1 belonged to CO-like gene family, and it might have the same function of controlling flowering time like other CO homologous genes.
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  • [1]

    Simpson G G, Dean C. Arabidopsis, the rosetta stone of flowering time [J]. Science, 2002, 296: 285-289
    [2]

    Mouradov A C F, Coupland G. Control of flowering time: interacting pathways as a basis for diversity [J]. Plant Cell, 2002, Suppl: 111-130
    [3]

    Datta S, Hettiarachchi G H C M, Deng X W, et al. Arabidopsis CONSTANS-LIKE3 is a positive regulator of red light signaling and root growth [J]. Plant Cell, 2006, 18 (1), 70-84
    [4]

    Miller T A, Muslin E H, Dorweiler J E. A maize CONSTANS-like gene, conz1, exhibits distinct diurnal expression patterns in varied photoperiods [J]. Planta, 2008, 227 (6): 1377-1388
    [5]

    Takahashi Y, Teshima K M, Yokoi S, et al. Variations in Hd1 proteins, Hd3a promoters, and Ehd1 expression levels contribute to diversity of flowering time in cultivated rice [J]. Proc Nation Acad Sci, 2009, 106 (11): 4555-4560
    [6]

    Putterill J, Robson F, Lee K, et al. The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors [J]. Cell, 1995, 80 (6): 847-857
    [7]

    Suarez-Lopez P, Wheatley K, Robson F, et al. CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis[J]. Nature, 2001, 410: 1116-1120
    [8]

    Nemoto Y, Kisaka M, Fuse T, et al. Characterization and functional analysis of three wheat genes with homology to the CONSTANS flowering time gene in transgenic rice [J]. Plant J, 2003, 36 (1): 82-93
    [9]

    Holefors A, OPseth L, Rosnes A K R, et al. Identification of PaCOL1 and PaCO2, two CONSTANS-like genes showing decreased transcript levels preceding short day induced growth cessation in Norway spruce [J]. Plant Physiol Biochem, 2009, 47 (2): 105-115
    [10]

    Almada R, Cabrera N, Casaretto J A, et al. VvCO and VvCOL1, two CONSTANS homologous genes, are regulated during flower induction and dormancy in grapevine buds [J]. Plant Cell Rep, 2009, 28 (8): 1193-1203
    [11]

    Hassidim M, Harir Y, Yakir E, et al. Over-expression of CONSTANS-LIKE5 can induce flowering in short-day grown Arabidopsis [J]. Planta, 2009, 230 (3): 481-491
    [12]

    Kim S Y, Yu X H, Michaels S D. Regulation of CONSTANS and FLOWERING LOCUS T expression in response to changing light quality [J]. Plant Physiology, 2008, 148 (1): 269-279
    [13] 辉朝茂, 杨宇明, 杜 凡, 等. 云南竹亚科珍稀种质资源及其开花结实研究[J]. 世界竹藤通讯, 2007, 5(2):15-20

    [14] 杜 凡,薛嘉榕,杨宇明,等.15年来云南竹子的开花现象及其类型的研究[J].林业科学, 2000, 36(6) : 57-68

    [15] 田 波.竹类植物开花时间相关基因的克隆与功能分析 . 昆明: 中国科学院昆明植物研究所, 2005

    [16] 王 泾.云南竹类开花规律研究及人工促进竹子开花的试验 .昆明: 西南林学院资源学院, 2005

    [17] 田 波, 陈永燕, 严远鑫, 等.一个竹类植物MADS区基因的克隆及其在拟南芥中的表达[J].科学通报, 2005, 50(2): 145-151

    [18]

    Mao W, Yang H Q, Li D Z. Dendrocalamus xishuangbannaensis (Poaceae:Bambusoideae), a new species from Yunnan, China [J]. Ann Bot Fennici, 2009, 46 (6): 574-576
    [19]

    Doyle J J, Doyle J L. A rapid DNA isolation procedure for small quantities of fresh leaf material [J]. Phytochem Bull, 1987, 19 (1): 11-15
    [20]

    Felsenstein, J. PHYLIP-Phylogeny Inference Package [J]. Cladistics, 1989, 5 (2): 164-166
    [21]

    Torok M, Elkin L D. Two B or not two B? Overview of the rapidly expanding B-box family of proteins [J]. Differentiation, 2000, 67 (1): 63-71
    [22]

    Strayer C, Oyama T, Schultz T F, et al. Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog [J]. Science, 2000, 289: 768-771
    [23]

    Robson F, Costa M, Hepworth S R, et al. Functional importance of conserved domains in the flowering-time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants [J]. Plant J, 2001, 28 (6): 619-631
    [24]

    Komeda Y. Genetic regulation of time to flower in Arabidopsis thaliana [J]. Annu Rev Plant Biol, 2004, 55:521-535
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Cloning and Sequence Analysis of CONSTANS Homologous Gene from Dendrocalamus xishuangbannaensis

  • 1. College of Biology Conservation, Southwest Forestry University, Kunming 650224, Yunnan, China
  • 2. Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China

Abstract: CONSTANS (CO) is one of the key genes controlling flowering time of the flowering plants in Photoperiod Pathways. In current study, two primers, COS1 and COA1, based on the conserved region of Hd1 sequences from CO-like gene of rice (Oryza sativa), were employed to amplify a 1 520 bp fragment by polymerase chain reaction (PCR) with genomic DNA of Dendrocalamus xishuangbannaensis D. Z. Li & H. Q. Yang. The fragment was cloned into a pGEM-T vector and then sequenced. Result of the 1 520 bp fragment cloned into a pGEM-T vector produced a fragment of gene, i.e. DxCO1, with accession number GQ358925 in GenBank. The DNA sequence analysis indicated that DxCO1 included one intron of 590 bp and two exons encoding putative 310 amino acids. Using homology analyses of DNA sequence, DxCO1 was homologous to the other six CO-like genes from Gramineae plants with 81%-91% supports, respectively. Phylogenetic analysis, based on protein sequences of DxCO1 and the known CO-like genes of seed plant in GenBank, showed that DxCO1 and other five CO-like genes from Gramineae plants clustered into a monophyly with bootstrap value of 92%. Meanwhile, there was a zinc-finger B-box (Cx2Cx8Cx7Cx2Cx4Hx8H) domain near amino terminus and a CCT (CO,CO-like, TOC1) domain near carboxy terminus in putative amino acid sequence of DxCO1. These results suggested that DxCO1 belonged to CO-like gene family, and it might have the same function of controlling flowering time like other CO homologous genes.

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