Effects of Waterlogging Stress on Growth and Physiological Characters in
Multiple Transgenic Populus× euramericana 'Guariento’

LI Huan; DING Chang-jun; SU Xiao-hua; SHEN Ying-bai; DU Ke-jiu

Volume 23 Issue 1

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Citation:

Effects of Waterlogging Stress on Growth and Physiological Characters in Multiple Transgenic Populus× euramericana 'Guariento’

1.
College of Forestry, Agricultural University of Hebei, Baoding 071001, Hebei, China
2.
Research Institute of Forestry, Chinese Academy of Forestry
3.
Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091,China

Abstract

Effects of different water treatments(waterlogging stress, light-waterlogging stress,normal water supply)on growth and physiology of multiple transgenic Populus× euramericana 'Guariento’(D5-9,D5-18,D5-19,D5-20,D5-21,D5-24,D5-26)were studied. The results showed that: Along with waterlogging stress intensifying, high growth, root growth and biomass accumulation of the multiple transgenic plants decreased, and the diameter of stem increased. Net photosynthetic rate decreased: The minimum was CK in the light-waterlogging stress treatment (11.99 μmol·m-2·s-1,14.0% lower than normal water supply) and the waterlogging stress treatment (10.37 μmol·m-2·s-1,25.6 % lower than normal water supply), whereas the maximum was D5-26(13.95 μmol·m-2·s-1) in the light-waterlogging stress treatment and D5-19(12.01 μmol·m-2·s-1) under waterlogging stress.Transpiration rate, water use efficiency, stomatal limit, chlorophyll a content, chlorophyll b content, total chlorophyll content, PSⅡ photochemical efficiency, electron transfer rate, potential fluorescence efficiency and maximum fluorescence efficiency of the multiple transgenic plants all decreased, but the reduction of chlorophyll a content was lower than chlorophyll b content. However, stomatal conductance, intercellular CO2, minimal fluorescence and maximum fluorescence increased. Waterlogging stress suppressed the growth, photosynthesis and chlorophyll fluorescence, and effects of the multiple transgenic plants were lighter and different. Growth, photosynthesis and chlorophyll fluorescence parameters were used to comprehensive evaluation of all the plants, the sequence of waterlogging resistance ability was: D5-26>D5-19>D5-18>D5-21>D5-9>D5-20>D5-24>CK.
- poplar,
- multiple transgenic,
- waterlogging,
- growth,
- photosynthesis,
- chlorophyllⅡ fluorescence

References

[1]	胡田田,康绍忠. 植物淹水胁迫响应的研究进展[J]. 福建农林大学学报:自然科学版,2005, 34(1):18-24
[2]	卓仁英, 陈益泰. 木本植物抗涝性研究进展[J]. 林业科学研究, 2001, 14(2): 215-222
[3]	Melissa S B, Katharine A P , Russell S. Influence of newly imposed salinity and waterlogging on Eucalyptus gracilis in South Australia[J]. Tree Physiology, 2005, 25: 1339-1346
[4]	Vicent Arbona, Zahed Hossain, María F, et al. Antioxidant enzymatic activity is linked to waterlogging stress tolerance in citrus[J] .Physiologia Plantarum, 2008, 132: 452-466
[5]	王生. 淹水胁迫对杨树无性系苗期生长及生理的影响[J]. 云南林业科技, 1998 (2): 28-33
[6]	曹福亮, 罗伯特·法门. 人工淹水处理对美洲黑杨生理特性的影响[J].南京林业大学学报, 1993,17(2):18-24
[7]	Wang J G, Su X H, JI L L, et al. Multiple transgenes Populus ×euramericana 'Guariento’ plants obtained by biolistic bombardment[J]. Chinese Science Bulletin, 2007, 52(2): 224-230
[8]	Maxwell K, Johnson G N. Chlorophyll fluorescence—apractical guide[J]. Journal of Experimental Botany, 2000,51:659-668
[9]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000: 134-137
[10]	王萍, 王荣, 刘庆华, 等. 几个牡丹品种耐涝性的比较[J]. 华中农业大学学报, 2008, 27(2): 309-312
[11]	杨敏生, 裴保华, 朱之悌. 白杨双交杂种株系抗旱性鉴定指标分析[J]. 林业科学, 2002, 38(6): 36-42
[12]	唐罗忠, 徐锡增, 方升佐. 土壤涝渍对杨树和柳树苗期生长及生理性状影响的研究[J]. 应用生态学报, 1998, 9(5): 471-474
[13]	Ahmed S, Nawata E, Hosokawa M, et al. Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging[J]. Plant Science, 2002, 163: 117-123
[14]	张培通, 徐立华, 杨长琴, 等. 涝渍对棉花产量及其构成的影响[J]. 江苏农业学报, 2008, 24(6): 785-791
[15]	Colmer T D. Aerenchyma and an inducible barrier to radial oxygen loss facilitate root aeration in upland, paddy and deep water rice[J]. Annals of Botany, 2003, 91: 301-309
[16]	Thomas A L,Guerreiro S M C,Sodek L.Aerenchyma formation and recovery from hypoxia of the flooded root system of nodulated soybean[J].Annals of Botany, 2005, 97: 1191-1198
[17]	李义良. 转基因杨树的分子检测及抗逆性评价 . 北京:北京林业大学, 2007
[18]	寇伟锋, 刘兆普, 陈铭达, 等. 不同浓度海水对油葵幼苗光合作用和叶绿素荧光特性的影响[J]. 西北植物学报, 2006, 26(1): 73-77
[19]	隆小华, 刘兆普, 蒋云芳, 等. 海水处理对不同产地菊芋幼苗光合作用及叶绿素荧光特性的影响[J]. 植物生态学报, 2006, 30(5): 827-834
[20]	Fernandez M D. Changes in photosynthesis and fluorescence in response to flooding in emerged and submerged leaves of Pouteria orinocoensis[J]. Phosynthetica, 2006, 44 (1): 32-38
[21]	陈芳清, 郭成圆, 王传华, 等. 水淹对秋华柳幼苗生理生态特征的影响[J]. 应用生态学报, 2008, 19(6): 1229-1233
[22]	惠红霞, 许兴, 李前荣. 外源甜菜碱对盐胁迫下枸杞光合功能的改善[J]. 西北植物学报, 2003, 23(12): 2137-2142
[23]	衣英华, 樊大勇, 谢宗强, 等. 模拟淹水对枫杨和栓皮栎气体交换、叶绿素荧光和水势的影响[J]. 植物生态学报, 2006, 30(6): 960-968
[24]	冯建灿, 胡秀丽, 毛训甲. 叶绿素荧光动力学在研究植物逆境生理中的应用[J].经济林研究, 2002, 20(4): 14-18, 30
[25]	罗俊, 张木清, 林彦铨, 等. 甘蔗苗期叶绿素荧光参数与抗旱性关系研究[J].中国农业科学, 2004, 37(11): 1718-1721
[26]	温国胜, 田海涛, 张明如, 等. 叶绿素荧光分析技术在林木培育中的应用[J]. 应用生态学报, 2006, 17(10): 1973-1977

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Effects of Waterlogging Stress on Growth and Physiological Characters in Multiple Transgenic Populus× euramericana 'Guariento’

1. College of Forestry, Agricultural University of Hebei, Baoding 071001, Hebei, China

2. Research Institute of Forestry, Chinese Academy of Forestry

3. Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091,China

Abstract: Effects of different water treatments(waterlogging stress, light-waterlogging stress,normal water supply)on growth and physiology of multiple transgenic Populus× euramericana 'Guariento’(D5-9,D5-18,D5-19,D5-20,D5-21,D5-24,D5-26)were studied. The results showed that: Along with waterlogging stress intensifying, high growth, root growth and biomass accumulation of the multiple transgenic plants decreased, and the diameter of stem increased. Net photosynthetic rate decreased: The minimum was CK in the light-waterlogging stress treatment (11.99 μmol·m-2·s-1,14.0% lower than normal water supply) and the waterlogging stress treatment (10.37 μmol·m-2·s-1,25.6 % lower than normal water supply), whereas the maximum was D5-26(13.95 μmol·m-2·s-1) in the light-waterlogging stress treatment and D5-19(12.01 μmol·m-2·s-1) under waterlogging stress.Transpiration rate, water use efficiency, stomatal limit, chlorophyll a content, chlorophyll b content, total chlorophyll content, PSⅡ photochemical efficiency, electron transfer rate, potential fluorescence efficiency and maximum fluorescence efficiency of the multiple transgenic plants all decreased, but the reduction of chlorophyll a content was lower than chlorophyll b content. However, stomatal conductance, intercellular CO2, minimal fluorescence and maximum fluorescence increased. Waterlogging stress suppressed the growth, photosynthesis and chlorophyll fluorescence, and effects of the multiple transgenic plants were lighter and different. Growth, photosynthesis and chlorophyll fluorescence parameters were used to comprehensive evaluation of all the plants, the sequence of waterlogging resistance ability was: D5-26>D5-19>D5-18>D5-21>D5-9>D5-20>D5-24>CK.

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Reference (26)

[1]	胡田田,康绍忠. 植物淹水胁迫响应的研究进展[J]. 福建农林大学学报:自然科学版,2005, 34(1):18-24
[2]	卓仁英, 陈益泰. 木本植物抗涝性研究进展[J]. 林业科学研究, 2001, 14(2): 215-222
[3]	Melissa S B, Katharine A P , Russell S. Influence of newly imposed salinity and waterlogging on Eucalyptus gracilis in South Australia[J]. Tree Physiology, 2005, 25: 1339-1346
[4]	Vicent Arbona, Zahed Hossain, María F, et al. Antioxidant enzymatic activity is linked to waterlogging stress tolerance in citrus[J] .Physiologia Plantarum, 2008, 132: 452-466
[5]	王生. 淹水胁迫对杨树无性系苗期生长及生理的影响[J]. 云南林业科技, 1998 (2): 28-33
[6]	曹福亮, 罗伯特·法门. 人工淹水处理对美洲黑杨生理特性的影响[J].南京林业大学学报, 1993,17(2):18-24
[7]	Wang J G, Su X H, JI L L, et al. Multiple transgenes Populus ×euramericana 'Guariento’ plants obtained by biolistic bombardment[J]. Chinese Science Bulletin, 2007, 52(2): 224-230
[8]	Maxwell K, Johnson G N. Chlorophyll fluorescence—apractical guide[J]. Journal of Experimental Botany, 2000,51:659-668
[9]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000: 134-137
[10]	王萍, 王荣, 刘庆华, 等. 几个牡丹品种耐涝性的比较[J]. 华中农业大学学报, 2008, 27(2): 309-312
[11]	杨敏生, 裴保华, 朱之悌. 白杨双交杂种株系抗旱性鉴定指标分析[J]. 林业科学, 2002, 38(6): 36-42
[12]	唐罗忠, 徐锡增, 方升佐. 土壤涝渍对杨树和柳树苗期生长及生理性状影响的研究[J]. 应用生态学报, 1998, 9(5): 471-474
[13]	Ahmed S, Nawata E, Hosokawa M, et al. Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging[J]. Plant Science, 2002, 163: 117-123
[14]	张培通, 徐立华, 杨长琴, 等. 涝渍对棉花产量及其构成的影响[J]. 江苏农业学报, 2008, 24(6): 785-791
[15]	Colmer T D. Aerenchyma and an inducible barrier to radial oxygen loss facilitate root aeration in upland, paddy and deep water rice[J]. Annals of Botany, 2003, 91: 301-309
[16]	Thomas A L,Guerreiro S M C,Sodek L.Aerenchyma formation and recovery from hypoxia of the flooded root system of nodulated soybean[J].Annals of Botany, 2005, 97: 1191-1198
[17]	李义良. 转基因杨树的分子检测及抗逆性评价 . 北京:北京林业大学, 2007
[18]	寇伟锋, 刘兆普, 陈铭达, 等. 不同浓度海水对油葵幼苗光合作用和叶绿素荧光特性的影响[J]. 西北植物学报, 2006, 26(1): 73-77
[19]	隆小华, 刘兆普, 蒋云芳, 等. 海水处理对不同产地菊芋幼苗光合作用及叶绿素荧光特性的影响[J]. 植物生态学报, 2006, 30(5): 827-834
[20]	Fernandez M D. Changes in photosynthesis and fluorescence in response to flooding in emerged and submerged leaves of Pouteria orinocoensis[J]. Phosynthetica, 2006, 44 (1): 32-38
[21]	陈芳清, 郭成圆, 王传华, 等. 水淹对秋华柳幼苗生理生态特征的影响[J]. 应用生态学报, 2008, 19(6): 1229-1233
[22]	惠红霞, 许兴, 李前荣. 外源甜菜碱对盐胁迫下枸杞光合功能的改善[J]. 西北植物学报, 2003, 23(12): 2137-2142
[23]	衣英华, 樊大勇, 谢宗强, 等. 模拟淹水对枫杨和栓皮栎气体交换、叶绿素荧光和水势的影响[J]. 植物生态学报, 2006, 30(6): 960-968
[24]	冯建灿, 胡秀丽, 毛训甲. 叶绿素荧光动力学在研究植物逆境生理中的应用[J].经济林研究, 2002, 20(4): 14-18, 30
[25]	罗俊, 张木清, 林彦铨, 等. 甘蔗苗期叶绿素荧光参数与抗旱性关系研究[J].中国农业科学, 2004, 37(11): 1718-1721
[26]	温国胜, 田海涛, 张明如, 等. 叶绿素荧光分析技术在林木培育中的应用[J]. 应用生态学报, 2006, 17(10): 1973-1977

Effects of Waterlogging Stress on Growth and Physiological Characters in Multiple Transgenic Populus× euramericana 'Guariento’

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通讯作者: 陈斌, bchen63@163.com

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Effects of Waterlogging Stress on Growth and Physiological Characters in Multiple Transgenic Populus× euramericana 'Guariento’

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