Studies on the Screening and Liquid Shake Culture Condition of High
Virulent Metarhizium anisopliae against Monochamus alternatus Hope Adults
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1.
Suqian Forestry Bureau of Jiangsu Province, Suqian 223800, Jiangsu,China
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2.
Forestry Academy of Jiangsu Province, Nanjing 211153,Jiangsu, China
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3.
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China
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Abstract
The germination percentage with different temperatures water bath treatment and virulent against Monochamus alternatus of different Metarhizium anisopliae were measured in this paper. The results showed that Metarhizium anisopliae789(Ma789)was the most virulent one. Conditions of liquid shake culture to Ma789 were studied by orthogonal test, the optimum liquid medium recipe was sucrose 20 g·L-1,yeast-extract paste 10 g·L-1,KH2PO4 3.0 g·L-1,MgSO4·7H2O 1.0 g·L-1,CaCl2 0.5 g·L-1 for mycelium culture; but for yielding spore, the optimum liquid medium recipe was sucrose 20 g·L-1, peptone 10 g·L-1,KH2PO4 1.5 g·L-1,MgSO4·7H2O 2.0 g·L-1,CaCl2 0.5 g·L-1.
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