Cloning and Expression Analysis of the C4H Gene Involved in the
Lignin Biosynthesis in Phyllostachys edulis

JIN Shun-yu; LU Meng-zhu; GAO jian

The full-length cDNA encoding C4H gene was cloned from 1-year-old moso bamboo seedings cDNAs using RT-PCR and RACE methods. The whole open reading frame C4H gene was 1 506 bp encoding 502 amino acids. The amino acid sequence shared high similarity with the corresponding genes in monocots Sorghum vulgare, Oryza sativa and Zea mays at 88%, 88% and 87% level, respectively. Real time quantitative PCR showed that expression of C4H was the highest in winter-shoot, moderate in top-spring shoot, mid-spring shoot, leaf, 3-year-old stem, root, leaf sheath, 2-year-old stem, whole spring shoot and 1-year-old stem, the lowest in base-shoot. This indicated that the cloned C4H was involved in the cell wall deposition during the development of vascular tissues, thus could be used in modulation of bamboo lignin biosynthesis through genetic engineering.

Cloning and Expression Analysis of the C4H Gene Involved in the Lignin Biosynthesis in Phyllostachys edulis

1. International Center of Bamboo and Rattan

2. Key Open Laboratory on Bamboo and Rattan Science and Technology,State Forestry Administration,Beijing 100102,China

Abstract: The full-length cDNA encoding C4H gene was cloned from 1-year-old moso bamboo seedings cDNAs using RT-PCR and RACE methods. The whole open reading frame C4H gene was 1 506 bp encoding 502 amino acids. The amino acid sequence shared high similarity with the corresponding genes in monocots Sorghum vulgare, Oryza sativa and Zea mays at 88%, 88% and 87% level, respectively. Real time quantitative PCR showed that expression of C4H was the highest in winter-shoot, moderate in top-spring shoot, mid-spring shoot, leaf, 3-year-old stem, root, leaf sheath, 2-year-old stem, whole spring shoot and 1-year-old stem, the lowest in base-shoot. This indicated that the cloned C4H was involved in the cell wall deposition during the development of vascular tissues, thus could be used in modulation of bamboo lignin biosynthesis through genetic engineering.

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Reference (19)

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Cloning and Expression Analysis of the C4H Gene Involved in the Lignin Biosynthesis in Phyllostachys edulis

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通讯作者: 陈斌, bchen63@163.com

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