The Study of High-Efficiency Plant Regeneration of Jatropha curcas

LIU Bo-bin; LU Meng-zhu; LI Ling; CHEN Jie-nan

The seedling leaves grown in greenhouse were used as explants to study the effects of different concentrations of 6-BA and IBA added in MS media on the regeneration of adventitious shoots. Cultured in the dark condition for 60 days, it is found that the optimal hormone combination for callus induction and adventitious shoots induction is 6-BA 5 mg·L-1 and IBA 0.5 mg·L-1. The induction rate of adventitious shoots is as high as 75.8%. Based on the optimal combination, the effect of gibberellic acid (GA3) on the regeneration of adventitious shoots was studied. The regeneration frequency of leaf-discs could reach to 90.9% and the average number of adventitious shoots was up to 4.6 when the concentration of GA3 was 0.05 mg·L-1.

1. Institute of Biological and Environmental Science &Technology, Central South University of Forestry and Technology, Changsha 410004, Hu’nan, China

2. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China)

Abstract: The seedling leaves grown in greenhouse were used as explants to study the effects of different concentrations of 6-BA and IBA added in MS media on the regeneration of adventitious shoots. Cultured in the dark condition for 60 days, it is found that the optimal hormone combination for callus induction and adventitious shoots induction is 6-BA 5 mg·L-1 and IBA 0.5 mg·L-1. The induction rate of adventitious shoots is as high as 75.8%. Based on the optimal combination, the effect of gibberellic acid (GA3) on the regeneration of adventitious shoots was studied. The regeneration frequency of leaf-discs could reach to 90.9% and the average number of adventitious shoots was up to 4.6 when the concentration of GA3 was 0.05 mg·L-1.

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Reference (12)

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The Study of High-Efficiency Plant Regeneration of Jatropha curcas

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