• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

Citation:

Preliminary Study on the Mortierella isabellina Fermentating to Produce Lipid

  • To improve the yield of microbial lipids, the lipids production was studied by using the fungus Mortierella isabellina. On the basis of the single factor experiment, the effect of various carbon and nitrogen sources and their concentrations, inoculum size, initial pH and inorganic ions on mycelium growth and accumulation of lipid yield in shaker culture was studied. The results showed that the optimal culture condition were as follows: glucose 100 g·L-1, yeast extract 3.0 g·L-1, inoculum size 20%, initial pH 5.0 - 6.0, MgSO4·7H2O 0.5 g·L-1, KH2PO4 2.0 g·L-1.Under the optimum culture condition, the biomass yield was 20.62 g·L-1, lipid content was 43.02%, the lipid yield was 8.87 g·L-1.
  • 加载中
  • [1]

    Hassan M, Philippe J B, Granger L M, et al. Influence of nitrogen and ion limitation on lipid production by crytococeus eurvatus grown in batch and fed-batch culture[J]. Process Biochemistry, 1996, 31(4): 355-361
    [2]

    Manirakiza P, Covaco A, Sehepens P. Comparative study on total lipid determination using aoxhlet, rcese-cottlieb, bli&dyer, and modified bligh&dyer extraction methoas[J]. Journal of Food Composition and Analysis, 2001, 14: 93-100
    [3]

    Papanikolaus S, Komaitis M, Aggelis G. Single cell oil (SCO) production by Mortierella isabellina grown on high-sugar content medial[J]. Bioresource Technology, 2004, 95: 287-291
    [4] 余 华. 微生物油脂开发利用的研究[J].粮油食品,2001,(3): 44-45

    [5]

    Hiruta O,Kamisaka Y,Yokochl T,et al. γ-linolenic acid production by a low temperature-resistant of Mortierella ramanniana[J]. Journal of Fermentation and Bioengineering, 1996,82(2): 119-123
    [6]

    Hiruta O,Kamisaka Y, Takebe H, et al. Optimization and scale-up of γ-linolenic acid production by Mortierella ramanniana MM 15-1, a high γ-linolenic acid producing mutant[J]. Journal of Fermentation and Bioengineering, 1996 ,82(4): 366-370
    [7] 张 鹏,杨培林,戴美学.微生物多不饱和脂肪酸的研究进展[J].微生物学杂志,2006,26(1):101-104

    [8] 黄亚东.γ-亚麻酸生产技术[J].广州食品工业科技,2002,18(1):28-29

    [9] 吴思国,赵 鑫,胡翠敏,等.转化N-乙酰-D-葡糖胺产油真菌的筛选[J].中国生物工程杂志,2008,28(11):58-62

    [10] 陈英明,陆继东,肖 波,等.生物柴油原料资源利用与开发[J].能源工程,2007(1):33-37

    [11] 宋安东,冯 冲,谢 慧,等.微生物技术在生物柴油开发与应用中的作用[J].食品与发酵工业,2006,32(10):93-97

    [12] 陶 杰,戴传超,戴 群,等.微生物能源的转化效率和经济可行性研究[J].微生物学杂志,2006,26(6):48-54

    [13] 李永红,刘 波,孙 艳,等.广谱碳源产油酵母的筛选[J].中国生物工程杂志,2005,25(12):39-44

    [14] 赵宗宝.加快微生物油脂研究为生物柴油产业提供廉价原料[J].中国生物工程杂志,2005,25(2):8-11.

    [15]

    Ratledge C.Regulation of lipid accumulation in oleaginous microorganisms[J].Biochem Sec Trans,2002,30:1047-1050
    [16] 郝 林.食品微生物学实验技术[M].北京:中国农业出版社,2001:138-139

    [17] 刘淑君,杨文博,施安辉.高产油脂酵母选育及摇瓶发酵条件的研究[J].微生物学通报,2000,27(2):93-97

    [18] 李植峰,张 玲,沈晓京,等.四种真菌油脂提取方法的比较研究[J].微生物学通报,2001,28(6):72-76

    [19] 楼献文.油脂中脂肪酸的气相毛细管色谱法分析[J].色谱,1993,11(6):346-347

    [20] 徐润娴,张 琦,吕宪禹.培养条件对少根根霉发酵生产γ-亚麻酸的影响[J].天津药学,2006,18(2):13-15

    [21]

    Ratledge C, Jame P W. The biochemistry and molecular biology of lipid accumulation in oleaginous microorgabisms[J]. Adv Appl Microbiol, 2002, 51: 1-51
    [22] 姚汝华.微生物工程工艺原理[M].广州:华南理工大学出版社,1996:194-195

    [23]

    Schafer A. Vegetable oil fattya acid methyl eaers as alternative diesel fuels for eommereial vehicles engines [J]. Plant Oil Fuel Proc Symp, 1987, 17 (4) : 29-46
    [24] 汤世华,陈明锴,杨建斌,等.深黄被饱霉高产多不饱和脂肪酸优化培养的研究[J].中国油脂,2008,33(5):37-39

  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article views(3750) PDF downloads(2467) Cited by()

Proportional views

Preliminary Study on the Mortierella isabellina Fermentating to Produce Lipid

  • 1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400,Zhejiang,China
  • 3. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China

Abstract: To improve the yield of microbial lipids, the lipids production was studied by using the fungus Mortierella isabellina. On the basis of the single factor experiment, the effect of various carbon and nitrogen sources and their concentrations, inoculum size, initial pH and inorganic ions on mycelium growth and accumulation of lipid yield in shaker culture was studied. The results showed that the optimal culture condition were as follows: glucose 100 g·L-1, yeast extract 3.0 g·L-1, inoculum size 20%, initial pH 5.0 - 6.0, MgSO4·7H2O 0.5 g·L-1, KH2PO4 2.0 g·L-1.Under the optimum culture condition, the biomass yield was 20.62 g·L-1, lipid content was 43.02%, the lipid yield was 8.87 g·L-1.

Reference (24)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return