Molecular Cloning, Characterization and Expression of Catalase1 Gene from Ginkgo biloba

CHENG Hua; LI Lin-ling; XU Feng; WANG Yan; CHENG Shui-yuan

Volume 23 Issue 4

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Molecular Cloning, Characterization and Expression of Catalase1 Gene from Ginkgo biloba

1.
College of Life Science and Engineering, Huanggang Normal University, Huanggang 438000, Hubei, China
3.
College of Horticulture Science, Hebei Agricultural University, Baoding 071001, Hebei, China

Abstract

The full-length cDNA sequence of Ginkgo biloba L. Catalase1 gene was isolated from G. biloba. Phylogenetic tree analysis revealed that GbCAT1 shared the same ancestor with other CAT. The result of Southern analysis showed that GbCAT1 gene was encoded by a small gene family in G. biloba. The expression analysis by RT-PCR showed that GbCAT1 expressed in a tissue-specific manner in G. biloba. GbCAT1 was also found to be up-regulated by the four tested abiotic stress, abscisic acid, osmotic stress, low temperature, and thermal injury. The expression of GbCAT1 was regulated to reduce by salicylic acid. These results indicate that the CAT1 has the potential to play a role in response to environmental stresses.
- Ginkgo biloba.,
- Catalase1 gene,
- expression analysis

References

[1]	Scandalios J G. Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses[J]. Brazilian Journal of Medical and Biological Research,2005, 38(7): 995-1014
[2]	Willekens H, Inzé D, Van Montagu M, et al. Catalases in plants[J]. Molecular Breeding,1995, 1(3): 207-228
[3]	Redinbaugh M G, Sabre M,Scandalios J G. The distribution of catalase activity, isozyme protein, and transcript in the tissues of the developing maize seedling[J]. Plant Physiology,1990, 92(2): 375-380
[4]	Lee S H,An C S. Differential expression of three catalase genes in hot pepper (Capsicum annuum L.)[J]. Molecules and cells,2005, 20(2): 247-255
[5]	Kwon S I, Lee H,An C S. Differential expression of three catalase genes in the small radish (Rhaphanus sativus L. var. sativus)[J]. Molecules and cells,2007, 24(1): 37-44
[6]	Boldt R,Scandalios J G. Influence of UV-light on the expression of the Cat2 and Cat3 catalase genes in maize[J]. Free Radical Biology and Medicine,1997, 23(3): 505-514
[7]	Chen Z, Iyer S, Caplan A, et al. Differential accumulation of salicylic acid and salicylic acid-sensitive catalase in different rice tissues[J]. Plant Physiology,1997, 114(1): 193-201
[8]	Eising R,Gerhardt B. Catalase synthesis and turnover during peroxisome transition in the cotyledons of Helianthus annuus L. [J]. Plant Physiology,1989, 89(3): 1000-1005
[9]	Esaka M, Yamada N, Kitabayashi M, et al. cDNA cloning and differential gene expression of three catalases in pumpkin[J]. Plant molecular biology,1997, 33(1): 141-155
[10]	Mittler R,Zilinskas B A. Purification and characterization of pea cytosolic ascorbate peroxidase[J]. Plant Physiology,1991, 97(3): 962-968
[11]	Mittler R,Zilinskas B A. Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought[J]. The Plant Journal,1994, 5(3): 397-405
[12]	Pinhero R G, Rao M V, Paliyath G, et al. Changes in activities of antioxidant enzymes and their relationship to genetic and paclobutrazol-induced chilling tolerance of maize seedlings[J]. Plant Physiology,1997, 114(2): 695-704
[13]	Esaka M, Maeshima M,Asahi T. Mechanism of the increase in catalase activity through microbody development in wounded sweet potato root tissue[J]. Plant and cell physiology,1983, 24(4): 615-623
[14]	Sakajo S, Nakamura K,Asahi T. Increase in catalase mRNA in wounded sweet potato tuberous root tissue[J]. Plant and cell physiology,1987, 28(5): 919-924
[15]	Badiani M, Schenone G, Paolacci A R, et al. Daily fluctuations of antioxidants in bean (Phaseolus vulgaris L.) leaves as affected by the presence of ambient air pollutants[J]. Plant and cell physiology,1993, 34(2): 271-279
[16]	Willekens H, Van Camp W, Van Montagu M, et al. Ozone, sulfur dioxide, and ultraviolet B have similar effects on mRNA accumulation of antioxidant genes in Nicotiana plumbaginifolia L[J]. Plant Physiology,1994, 106(3): 1007-1014
[17]	Prasad T K, Anderson M D, Martin B A, et al. Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide[J]. The Plant Cell Online,1994, 6(1):65-74
[18]	Mandhania S, Madan S,Sawhney V. Antioxidant defense mechanism under salt stress in wheat seedlings[J]. Biologia Plantarum,2006, 50(2): 227-231
[19]	Dat J F, Pellinen R, Beeckman T, et al. Changes in hydrogen peroxide homeostasis trigger an active cell death process in tobacco[J]. Plant Journal,2003, 33(4): 621-632
[20]	Polidoros A N, Mylona P V,Scandalios J G. Transgenic tobacco plants expressing the maize Cat2 gene have altered catalase levels that affect plant-pathogen interactions and resistance to oxidative stress[J]. Transgenic Research,2001,10(6): 555-569
[21]	Mohamed E A, Iwaki T, Munir I, et al. Overexpression of bacterial catalase in tomato leaf chloroplasts enhances photo-oxidative stress tolerance[J]. Plant, Cell and Environment,2003, 26(2):2037-2046
[22]	Deng Z, Wang Y, Jiang K, et al. Molecular cloning and characterization of a novel dehydrin gene from Ginkgo biloba[J]. Bioscience Reports,2006, 26(3): 203-215
[23]	魏春红,李毅. 现代分子生物学实验[M]. 北京: 高等教育出版社, 2006: 135-137
[24]	蔡荣,许锋,陈柳吉,等. 银杏不同组织的总RNA提取方法的改进[J]. 生物技术,2007, 17(4): 38-40
[25]	Jansson S, Meyer-Gauen G, Cerff R, et al. Nucleotide distribution in gymnosperm nuclear sequences suggests a model for GC-content change in land-plant nuclear genomes[J]. Journal of molecular evolution,1994, 39(1): 34-46
[26]	Redinbaugh M G, Wadsworth G J,Scandalios J G. Characterization of catalase transcripts and their differential expression in maize[J]. Biochimica et biophysica acta,1988, 951(1): 104
[27]	Kunce C M,Trelease R N. Heterogeneity of catalase in maturing and germinated cotton seeds 1[J]. Plant Physiology,1986, 81(4): 1134-1139
[28]	Willekens H, Langebartels C,Tire C. Differential expression of catalase gene in Nicotiana plumbaginifolia[J]. PNAS,1994, 91(22): 10450-10454
[29]	Guan L, Zhao J,Scandalios J G. Cis-elements and trans-factors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress: H₂O₂ is likely intermediary signaling molecule for the response[J]. Plant Journal,2000, 22(2):87-95
[30]	Zhang A, Jiang M, Zhang J, et al. Mitogen-activated protein kinase is involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants[J]. Plant Physiology,2006, 141(2): 475
[31]	Gaffney T, Friedrich L, Vernooij B, et al. Requirement of salicylic acid for the induction of systemic acquired resistance[J]. Science,1993, 261(5122): 754-756
[32]	Gorlach J, Volrath S, Knauf-Beiter G, et al. Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat[J]. The Plant Cell,1996, 8(4): 629-643
[33]	Dat J F, Lopez-Delgado H, Foyer C H, et al. Parallel changes in H₂O₂ and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings[J]. Plant Physiology,1998, 116(4): 1351-1357
[34]	Janda T, Szalai G, Tari I, et al. Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants[J]. Planta,1999, 208(2): 175-180
[35]	Chen Z, Silva H,Klessig D F. Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid[J]. Science,1993, 262(5141): 1883-1886
[36]	王晓娟,倪建福. 盐胁迫下小麦新品系89122的抗氧化酶类活性变化的研究[J]. 兰州大学学报,1999, 35(1): 140-144
[37]	Yang W L, Liu J M, Chen F, et al. Identification of Festuca arundinacea schreb Cat1 catalase gene and analysis of its expression under abiotic Stresses[J]. Journal of Integrative Plant Biology,2006, 48(3): 334-340
[38]	Goulas E, Schubert M, Kieselbach T, et al. The chloroplast lumen and stromal proteomes of Arabidopsis thaliana show differential sensitivity to short-and long-term exposure to low temperature[J]. The Plant Journal,2006, 47(5): 720-734
[39]	Kim J Y, Park S J, Jang B, et al. Functional characterization of a glycine-rich RNA-binding protein 2 in Arabidopsis thaliana under abiotic stress conditions[J]. Plant Journal,2007, 50(3): 439
[40]	刘汉梅, 张怀渝, 谭振波, 等. 玉米Catalase-3基因克隆及低温表达研究[J]. 四川农业大学学报,2006, 24(3): 272-275
[41]	Rainwater D T, Gossetp D R, Millhollon E P, et al. The relationship between yield and the antioxidant defense system in tomatoes grown under heat stress[J]. Free Radical Research,1996, 25(5): 421-435
[42]	Feierabend J, Schaan C,Hertwig B. Photoinactivation of catalase occurs under both high-and low-temperature stress conditions and accompanies photoinhibition of photosystem II [J]. Plant Physiology,1992, 100(3): 1554-1561

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Molecular Cloning, Characterization and Expression of Catalase1 Gene from Ginkgo biloba

1. College of Life Science and Engineering, Huanggang Normal University, Huanggang 438000, Hubei, China

3. College of Horticulture Science, Hebei Agricultural University, Baoding 071001, Hebei, China

Abstract: The full-length cDNA sequence of Ginkgo biloba L. Catalase1 gene was isolated from G. biloba. Phylogenetic tree analysis revealed that GbCAT1 shared the same ancestor with other CAT. The result of Southern analysis showed that GbCAT1 gene was encoded by a small gene family in G. biloba. The expression analysis by RT-PCR showed that GbCAT1 expressed in a tissue-specific manner in G. biloba. GbCAT1 was also found to be up-regulated by the four tested abiotic stress, abscisic acid, osmotic stress, low temperature, and thermal injury. The expression of GbCAT1 was regulated to reduce by salicylic acid. These results indicate that the CAT1 has the potential to play a role in response to environmental stresses.

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Reference (42)

[1]	Scandalios J G. Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses[J]. Brazilian Journal of Medical and Biological Research,2005, 38(7): 995-1014
[2]	Willekens H, Inzé D, Van Montagu M, et al. Catalases in plants[J]. Molecular Breeding,1995, 1(3): 207-228
[3]	Redinbaugh M G, Sabre M,Scandalios J G. The distribution of catalase activity, isozyme protein, and transcript in the tissues of the developing maize seedling[J]. Plant Physiology,1990, 92(2): 375-380
[4]	Lee S H,An C S. Differential expression of three catalase genes in hot pepper (Capsicum annuum L.)[J]. Molecules and cells,2005, 20(2): 247-255
[5]	Kwon S I, Lee H,An C S. Differential expression of three catalase genes in the small radish (Rhaphanus sativus L. var. sativus)[J]. Molecules and cells,2007, 24(1): 37-44
[6]	Boldt R,Scandalios J G. Influence of UV-light on the expression of the Cat2 and Cat3 catalase genes in maize[J]. Free Radical Biology and Medicine,1997, 23(3): 505-514
[7]	Chen Z, Iyer S, Caplan A, et al. Differential accumulation of salicylic acid and salicylic acid-sensitive catalase in different rice tissues[J]. Plant Physiology,1997, 114(1): 193-201
[8]	Eising R,Gerhardt B. Catalase synthesis and turnover during peroxisome transition in the cotyledons of Helianthus annuus L. [J]. Plant Physiology,1989, 89(3): 1000-1005
[9]	Esaka M, Yamada N, Kitabayashi M, et al. cDNA cloning and differential gene expression of three catalases in pumpkin[J]. Plant molecular biology,1997, 33(1): 141-155
[10]	Mittler R,Zilinskas B A. Purification and characterization of pea cytosolic ascorbate peroxidase[J]. Plant Physiology,1991, 97(3): 962-968
[11]	Mittler R,Zilinskas B A. Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought[J]. The Plant Journal,1994, 5(3): 397-405
[12]	Pinhero R G, Rao M V, Paliyath G, et al. Changes in activities of antioxidant enzymes and their relationship to genetic and paclobutrazol-induced chilling tolerance of maize seedlings[J]. Plant Physiology,1997, 114(2): 695-704
[13]	Esaka M, Maeshima M,Asahi T. Mechanism of the increase in catalase activity through microbody development in wounded sweet potato root tissue[J]. Plant and cell physiology,1983, 24(4): 615-623
[14]	Sakajo S, Nakamura K,Asahi T. Increase in catalase mRNA in wounded sweet potato tuberous root tissue[J]. Plant and cell physiology,1987, 28(5): 919-924
[15]	Badiani M, Schenone G, Paolacci A R, et al. Daily fluctuations of antioxidants in bean (Phaseolus vulgaris L.) leaves as affected by the presence of ambient air pollutants[J]. Plant and cell physiology,1993, 34(2): 271-279
[16]	Willekens H, Van Camp W, Van Montagu M, et al. Ozone, sulfur dioxide, and ultraviolet B have similar effects on mRNA accumulation of antioxidant genes in Nicotiana plumbaginifolia L[J]. Plant Physiology,1994, 106(3): 1007-1014
[17]	Prasad T K, Anderson M D, Martin B A, et al. Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide[J]. The Plant Cell Online,1994, 6(1):65-74
[18]	Mandhania S, Madan S,Sawhney V. Antioxidant defense mechanism under salt stress in wheat seedlings[J]. Biologia Plantarum,2006, 50(2): 227-231
[19]	Dat J F, Pellinen R, Beeckman T, et al. Changes in hydrogen peroxide homeostasis trigger an active cell death process in tobacco[J]. Plant Journal,2003, 33(4): 621-632
[20]	Polidoros A N, Mylona P V,Scandalios J G. Transgenic tobacco plants expressing the maize Cat2 gene have altered catalase levels that affect plant-pathogen interactions and resistance to oxidative stress[J]. Transgenic Research,2001,10(6): 555-569
[21]	Mohamed E A, Iwaki T, Munir I, et al. Overexpression of bacterial catalase in tomato leaf chloroplasts enhances photo-oxidative stress tolerance[J]. Plant, Cell and Environment,2003, 26(2):2037-2046
[22]	Deng Z, Wang Y, Jiang K, et al. Molecular cloning and characterization of a novel dehydrin gene from Ginkgo biloba[J]. Bioscience Reports,2006, 26(3): 203-215
[23]	魏春红,李毅. 现代分子生物学实验[M]. 北京: 高等教育出版社, 2006: 135-137
[24]	蔡荣,许锋,陈柳吉,等. 银杏不同组织的总RNA提取方法的改进[J]. 生物技术,2007, 17(4): 38-40
[25]	Jansson S, Meyer-Gauen G, Cerff R, et al. Nucleotide distribution in gymnosperm nuclear sequences suggests a model for GC-content change in land-plant nuclear genomes[J]. Journal of molecular evolution,1994, 39(1): 34-46
[26]	Redinbaugh M G, Wadsworth G J,Scandalios J G. Characterization of catalase transcripts and their differential expression in maize[J]. Biochimica et biophysica acta,1988, 951(1): 104
[27]	Kunce C M,Trelease R N. Heterogeneity of catalase in maturing and germinated cotton seeds 1[J]. Plant Physiology,1986, 81(4): 1134-1139
[28]	Willekens H, Langebartels C,Tire C. Differential expression of catalase gene in Nicotiana plumbaginifolia[J]. PNAS,1994, 91(22): 10450-10454
[29]	Guan L, Zhao J,Scandalios J G. Cis-elements and trans-factors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress: H₂O₂ is likely intermediary signaling molecule for the response[J]. Plant Journal,2000, 22(2):87-95
[30]	Zhang A, Jiang M, Zhang J, et al. Mitogen-activated protein kinase is involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants[J]. Plant Physiology,2006, 141(2): 475
[31]	Gaffney T, Friedrich L, Vernooij B, et al. Requirement of salicylic acid for the induction of systemic acquired resistance[J]. Science,1993, 261(5122): 754-756
[32]	Gorlach J, Volrath S, Knauf-Beiter G, et al. Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat[J]. The Plant Cell,1996, 8(4): 629-643
[33]	Dat J F, Lopez-Delgado H, Foyer C H, et al. Parallel changes in H₂O₂ and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings[J]. Plant Physiology,1998, 116(4): 1351-1357
[34]	Janda T, Szalai G, Tari I, et al. Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants[J]. Planta,1999, 208(2): 175-180
[35]	Chen Z, Silva H,Klessig D F. Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid[J]. Science,1993, 262(5141): 1883-1886
[36]	王晓娟,倪建福. 盐胁迫下小麦新品系89122的抗氧化酶类活性变化的研究[J]. 兰州大学学报,1999, 35(1): 140-144
[37]	Yang W L, Liu J M, Chen F, et al. Identification of Festuca arundinacea schreb Cat1 catalase gene and analysis of its expression under abiotic Stresses[J]. Journal of Integrative Plant Biology,2006, 48(3): 334-340
[38]	Goulas E, Schubert M, Kieselbach T, et al. The chloroplast lumen and stromal proteomes of Arabidopsis thaliana show differential sensitivity to short-and long-term exposure to low temperature[J]. The Plant Journal,2006, 47(5): 720-734
[39]	Kim J Y, Park S J, Jang B, et al. Functional characterization of a glycine-rich RNA-binding protein 2 in Arabidopsis thaliana under abiotic stress conditions[J]. Plant Journal,2007, 50(3): 439
[40]	刘汉梅, 张怀渝, 谭振波, 等. 玉米Catalase-3基因克隆及低温表达研究[J]. 四川农业大学学报,2006, 24(3): 272-275
[41]	Rainwater D T, Gossetp D R, Millhollon E P, et al. The relationship between yield and the antioxidant defense system in tomatoes grown under heat stress[J]. Free Radical Research,1996, 25(5): 421-435
[42]	Feierabend J, Schaan C,Hertwig B. Photoinactivation of catalase occurs under both high-and low-temperature stress conditions and accompanies photoinhibition of photosystem II [J]. Plant Physiology,1992, 100(3): 1554-1561

Molecular Cloning, Characterization and Expression of Catalase1 Gene from Ginkgo biloba

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通讯作者: 陈斌, bchen63@163.com

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Molecular Cloning, Characterization and Expression of Catalase1 Gene from Ginkgo biloba

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