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Citation:

FISH-AFLP Analysis of Genetic Diversity of Early-fruiting Walnut Cultivars

  • Received Date: 2008-11-18
  • 16 early-fruiting walnut (Juglans regia L.) cultivars were developed in China in 1994. These new walnut cultivars preliminarily formed an improved variety system for Chinese walnut production. The FISH-AFLP analysis was conducted in order to investigate the sibship among the 16 Chinese early-fruiting walnut cultivars. 9 primer combinations were used to amplify the genomic DNAs. Total 1 072 AFLP fragments were obtained, in which 946 are polymorphic markers and the average percent of polymorphic bands is 88.12%. Different genotypes were detected and unique bands were found. The genetic diversity and their sibship among Chinese early-fruiting walnut cultivars were investigated and their fingerprintings were established in the research.
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FISH-AFLP Analysis of Genetic Diversity of Early-fruiting Walnut Cultivars

  • 1. Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China

Abstract: 16 early-fruiting walnut (Juglans regia L.) cultivars were developed in China in 1994. These new walnut cultivars preliminarily formed an improved variety system for Chinese walnut production. The FISH-AFLP analysis was conducted in order to investigate the sibship among the 16 Chinese early-fruiting walnut cultivars. 9 primer combinations were used to amplify the genomic DNAs. Total 1 072 AFLP fragments were obtained, in which 946 are polymorphic markers and the average percent of polymorphic bands is 88.12%. Different genotypes were detected and unique bands were found. The genetic diversity and their sibship among Chinese early-fruiting walnut cultivars were investigated and their fingerprintings were established in the research.

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