Effects of Long-term Cryopreservation on the Cytoactive of Two Insect Cell Lines SL2 and NIH-SaPe-4
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1.
Research Institute of Resource Insects, Chinese Academy of Forestry,Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration,Kunming 650224, Yunnan, China
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Received Date:
2010-05-10
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Abstract
In order to evaluate the effects of different cryoprotectants and durations on post-thaw cytoactives (including cell viability, circularity and recovery time) of two dipteral insect cell lines, 3 kinds of cryoprotectants (Formula I: 10% DMSO + 90% FBS; Formula II: 10% DMSO; Formula III: 10% glycerol) were used to preserve the cells of SL2 from embryo of Drosophila melanogaster and NIH-SaPe-4 from embryo of Sarcophaga peregrine in liquid nitrogen separately. The results showed that the post-thaw viability of these two cell lines decreased with the cryopreservation time expended. In long-term cryopreservation, the effect of Formula I for preserving SL2 cells was better than the others. Formula II was suitable for NIH-SaPe-4. Using glycerol as cryoprotectant was unsuitable for these two insect cell lines.
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