Study on Physiological and Biochemical Responses of Camellia oleifera to Low Phosphorus Stress

CHEN Long-sheng; CHEN Yong-zhong; PENG Shao-feng; MA Li; WANG Rui; WANG Xiang-nan

Volume 23 Issue 5

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Study on Physiological and Biochemical Responses of Camellia oleifera to Low Phosphorus Stress

1.
Hunan Academy of Forestry, Changsha 410004,Hunan,China

Received Date: 2010-03-10

Abstract

The physiological and biochemical responses of Camellia oleifera to low phosphorus stress were investigated in this study, and the different phosphorus concentrations of the treatments were conducted by using a single factor experiment with water culture in the greenhouse. Results showed that the activity of APA in leaves tissues and those secreted by the roots all increased under P deficiency, which respectively increased by 16.49% - 86.47% and 28.7% - 58.7% compared with those of the control. The increase of APA may be an active adaptive response of Camellia oleifera to low phosphorus stress.Under the situation of low phosphorus stress, the concentrations of soluble sugar and free proline increased whereas the soluble protein decreased,those showed that normal protein synthesis and carbohydrate metabolism were blocked under the situation of low phosphorus stress; Moreover, the homeostasis established by the increase of MDA concent and protective enzyme (SOD) activity in leaves was the mechanism of the adaptive response for Camellia oleifera to low phosphorus stress.
- Camellia oleifera,
- low phosphorus stress,
- physiological and biochemical responses

References

[1]	Epsein E. Mineral Nurtition fo Plants[M]. NewYork: J Wiley and Sons, Inc, 1972,51-56
[2]	万美亮.缺磷胁迫对甘蔗过氧化及保护酶系统活性的影响[J].华南农业大学学报,1999,20(2):131-135
[3]	张福锁.植物营养生态生理学和遗传学[M].北京:中国科学技术出版社,1993:53-63
[4]	周志春,谢钰容,金国庆,等.马尾松种源对磷肥的遗传反应及根际土壤营养差异[J].林业科学,2003,39(6):62-67
[5]	Batten G D. A review of phosphorus efficiency in wheat[J]. Plant and Soil, 1992,146:163-168
[6]	何方,何柏.油茶栽培分布与立地分类的研究[J].林业科学,2002,38(5): 64 -73
[7]	徐向华,丁贵杰.马尾松适应低磷胁迫的生理生化响应[J].林业科学,2006,42(9):24-28
[8]	Baldwin J C, Karthikeyan A S, Raghothama K G. Aphosphorus starvation induced novel acid phosphatase from tomato[J].Plant Physiol,2001,125:728-737
[9]	Mitsukawa N, Okumura S, Shirano Y, et al. Over expression of an Arabidopsis thaliana high affinity phosphate transporter genein tobacco cultured cells enhances cell growth underphosphate limited conditions[J].Pro Natl Acad Sci,1997,94: 7098-7102
[10]	Smith F W, Ealing P M, Dong B, et al.The cloning of two Arabidopsisgenes belonging to a phosphate[J]. Transporter Family Plant J,1997,11:83-92
[11]	Diem H G, Duhoux E, Zaid H, et al.Cluster roots in Casuari-naceae:Role and relationship to soil nutrient factors[J].Ann Bot,2000,85:929 - 936
[12]	Gilbert G A, Knight D, Vance C P. Proteoid root development of phosphorus deficient lupin is mimicked by auxin and phosphonate[J].Ann Bot,2000,85:921-928
[13]	Neumann G, Massonneau A, Langlade N, et al.Physiological aspects of cluster root function and development in phosphorus-deficient white lupin (Lupinus albus L.) [J].Ann Bot,2000,85:909 - 919
[14]	Dinkelaker B, Hengeler C, Marschner H. Distribution and function of proteoid roots and other root clusters [J].Bot Acta,1995,108:183-200
[15]	Grierson P F. Organic acids in the rhizosphere of Banksia interifolia L.F[J].Plant Soil,1992,44:259-265
[16]	Whitehead S J, Caporn S J M, Press M C. Effects of elevated CO₂, nitrogen and phosphorus on the growth and photosynthesis of two upland perennials:Calluna vulgaris and Pteridium aquilinum[J].New Phytol,1997,135:201-211
[17]	Rao I M, Freeden A L, Terry N. Influence of phosphorus limitation on photosynthesis,carbon allocation and partitioning in sugarbeet soybean grown with a short photoperiod[J].Plant Physiol Biochem,1993,31:223-231
[18]	张志良.植物生理学实验指导[M].北京:高等教育出版社,1990
[19]	邹琦.植物生理生化实验指导[M].北京:中国农业出版社,1995
[20]	中国科学院上海植物生理研究所.现代植物生理实验指导[M].上海:上海科学技术出版社,1999,305-306
[21]	Mclanchlan K D. Acid phosphatase activity of intact roots and phosphorus nustrition in plants (Ⅰ): Assay conditions and phosphatase activity[J].Aust J Agric Res, 1980, 31: 429-440
[22]	林启美,黄德明.应用酸性磷酸酶进行番茄磷素诊断[J].华北农学报,1991,6:78-83
[23]	潘瑞帜. 植物生理学[M].北京:高等教育出版社,2004
[24]	王伟,曹敏建,王晓光,等.低钾胁迫对不同钾营养效应型大豆保护酶系统的影响[J].大豆科学,2005,24(2):102-104
[25]	刘厚诚,邝炎华,陈日远.缺磷胁迫下长豇豆幼苗膜脂过氧化及保护酶活性的变化[J].园艺学报,2003,30(2):215-217
[26]	潘晓华,刘水英,李锋,等.低磷胁迫对不同水稻品种叶片膜脂过氧化及保护酶活性的影响[J].中国水稻科学,2003,17(1):57-60
[27]	宋克敏,焦新之,李琳,等.磷酸饥饿时番茄幼苗酸性磷酸酶活性的变化与Pi吸收的关系[J].云南植物研究, 1999,21(1): 101-108
[28]	Dracup M N H, Barrett E G, Greeway H, et al. Effect of phosphorus deficiency on phosphatase activity of cellwalls from roots of subterranean clover[J].J Exp Bot, 1984, 35:466-480
[29]	陈永亮,李修岭,周晓燕.低磷胁迫对落叶松幼苗生长及根系酸性磷酸酶活性的影响[J].北京林业大学学报,2006,28(6):46-50
[30]	陈佰岩,郑毅,汤利.磷胁迫条件下小麦、蚕豆根系分泌物对红壤磷的活化[J].云南农业大学学报,2009,24(6):869-875
[31]	俞元春,余健,房莉,等.缺磷胁迫下马尾松和杉木苗根系有机酸的分泌[J].南京林业大学学报,2007,31(2):9-11
[32]	梁霞,刘爱琴,马样庆,等. 磷胁迫对不同杉木无性系酸性磷酸酶活性的影响[J].植物生态学报,2005,29(1):54-59
[33]	Helal H M. Varietal difference in roots phosphatase activity asrelated to the utilization of organic phosphorus[M]//Bassam N. Genetic Aspects of Plant Mineral Nutrition. Netherlands: Kluwer Academic Publishers, 1990

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Study on Physiological and Biochemical Responses of Camellia oleifera to Low Phosphorus Stress

1. Hunan Academy of Forestry, Changsha 410004,Hunan,China

Received Date: 2010-03-10

Abstract: The physiological and biochemical responses of Camellia oleifera to low phosphorus stress were investigated in this study, and the different phosphorus concentrations of the treatments were conducted by using a single factor experiment with water culture in the greenhouse. Results showed that the activity of APA in leaves tissues and those secreted by the roots all increased under P deficiency, which respectively increased by 16.49% - 86.47% and 28.7% - 58.7% compared with those of the control. The increase of APA may be an active adaptive response of Camellia oleifera to low phosphorus stress.Under the situation of low phosphorus stress, the concentrations of soluble sugar and free proline increased whereas the soluble protein decreased,those showed that normal protein synthesis and carbohydrate metabolism were blocked under the situation of low phosphorus stress; Moreover, the homeostasis established by the increase of MDA concent and protective enzyme (SOD) activity in leaves was the mechanism of the adaptive response for Camellia oleifera to low phosphorus stress.

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Reference (33)

[1]	Epsein E. Mineral Nurtition fo Plants[M]. NewYork: J Wiley and Sons, Inc, 1972,51-56
[2]	万美亮.缺磷胁迫对甘蔗过氧化及保护酶系统活性的影响[J].华南农业大学学报,1999,20(2):131-135
[3]	张福锁.植物营养生态生理学和遗传学[M].北京:中国科学技术出版社,1993:53-63
[4]	周志春,谢钰容,金国庆,等.马尾松种源对磷肥的遗传反应及根际土壤营养差异[J].林业科学,2003,39(6):62-67
[5]	Batten G D. A review of phosphorus efficiency in wheat[J]. Plant and Soil, 1992,146:163-168
[6]	何方,何柏.油茶栽培分布与立地分类的研究[J].林业科学,2002,38(5): 64 -73
[7]	徐向华,丁贵杰.马尾松适应低磷胁迫的生理生化响应[J].林业科学,2006,42(9):24-28
[8]	Baldwin J C, Karthikeyan A S, Raghothama K G. Aphosphorus starvation induced novel acid phosphatase from tomato[J].Plant Physiol,2001,125:728-737
[9]	Mitsukawa N, Okumura S, Shirano Y, et al. Over expression of an Arabidopsis thaliana high affinity phosphate transporter genein tobacco cultured cells enhances cell growth underphosphate limited conditions[J].Pro Natl Acad Sci,1997,94: 7098-7102
[10]	Smith F W, Ealing P M, Dong B, et al.The cloning of two Arabidopsisgenes belonging to a phosphate[J]. Transporter Family Plant J,1997,11:83-92
[11]	Diem H G, Duhoux E, Zaid H, et al.Cluster roots in Casuari-naceae:Role and relationship to soil nutrient factors[J].Ann Bot,2000,85:929 - 936
[12]	Gilbert G A, Knight D, Vance C P. Proteoid root development of phosphorus deficient lupin is mimicked by auxin and phosphonate[J].Ann Bot,2000,85:921-928
[13]	Neumann G, Massonneau A, Langlade N, et al.Physiological aspects of cluster root function and development in phosphorus-deficient white lupin (Lupinus albus L.) [J].Ann Bot,2000,85:909 - 919
[14]	Dinkelaker B, Hengeler C, Marschner H. Distribution and function of proteoid roots and other root clusters [J].Bot Acta,1995,108:183-200
[15]	Grierson P F. Organic acids in the rhizosphere of Banksia interifolia L.F[J].Plant Soil,1992,44:259-265
[16]	Whitehead S J, Caporn S J M, Press M C. Effects of elevated CO₂, nitrogen and phosphorus on the growth and photosynthesis of two upland perennials:Calluna vulgaris and Pteridium aquilinum[J].New Phytol,1997,135:201-211
[17]	Rao I M, Freeden A L, Terry N. Influence of phosphorus limitation on photosynthesis,carbon allocation and partitioning in sugarbeet soybean grown with a short photoperiod[J].Plant Physiol Biochem,1993,31:223-231
[18]	张志良.植物生理学实验指导[M].北京:高等教育出版社,1990
[19]	邹琦.植物生理生化实验指导[M].北京:中国农业出版社,1995
[20]	中国科学院上海植物生理研究所.现代植物生理实验指导[M].上海:上海科学技术出版社,1999,305-306
[21]	Mclanchlan K D. Acid phosphatase activity of intact roots and phosphorus nustrition in plants (Ⅰ): Assay conditions and phosphatase activity[J].Aust J Agric Res, 1980, 31: 429-440
[22]	林启美,黄德明.应用酸性磷酸酶进行番茄磷素诊断[J].华北农学报,1991,6:78-83
[23]	潘瑞帜. 植物生理学[M].北京:高等教育出版社,2004
[24]	王伟,曹敏建,王晓光,等.低钾胁迫对不同钾营养效应型大豆保护酶系统的影响[J].大豆科学,2005,24(2):102-104
[25]	刘厚诚,邝炎华,陈日远.缺磷胁迫下长豇豆幼苗膜脂过氧化及保护酶活性的变化[J].园艺学报,2003,30(2):215-217
[26]	潘晓华,刘水英,李锋,等.低磷胁迫对不同水稻品种叶片膜脂过氧化及保护酶活性的影响[J].中国水稻科学,2003,17(1):57-60
[27]	宋克敏,焦新之,李琳,等.磷酸饥饿时番茄幼苗酸性磷酸酶活性的变化与Pi吸收的关系[J].云南植物研究, 1999,21(1): 101-108
[28]	Dracup M N H, Barrett E G, Greeway H, et al. Effect of phosphorus deficiency on phosphatase activity of cellwalls from roots of subterranean clover[J].J Exp Bot, 1984, 35:466-480
[29]	陈永亮,李修岭,周晓燕.低磷胁迫对落叶松幼苗生长及根系酸性磷酸酶活性的影响[J].北京林业大学学报,2006,28(6):46-50
[30]	陈佰岩,郑毅,汤利.磷胁迫条件下小麦、蚕豆根系分泌物对红壤磷的活化[J].云南农业大学学报,2009,24(6):869-875
[31]	俞元春,余健,房莉,等.缺磷胁迫下马尾松和杉木苗根系有机酸的分泌[J].南京林业大学学报,2007,31(2):9-11
[32]	梁霞,刘爱琴,马样庆,等. 磷胁迫对不同杉木无性系酸性磷酸酶活性的影响[J].植物生态学报,2005,29(1):54-59
[33]	Helal H M. Varietal difference in roots phosphatase activity asrelated to the utilization of organic phosphorus[M]//Bassam N. Genetic Aspects of Plant Mineral Nutrition. Netherlands: Kluwer Academic Publishers, 1990

Study on Physiological and Biochemical Responses of Camellia oleifera to Low Phosphorus Stress

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通讯作者: 陈斌, bchen63@163.com

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Study on Physiological and Biochemical Responses of Camellia oleifera to Low Phosphorus Stress

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