Molecular Detection and Sequence Analysis of wsp Gene from Wolbachia  in Metaphycus ericeri Xu et Jiang in Ericerus pela

YANG Pu; CHEN Xiao-ming; LI Meng; WEN Can

Universal primers were used to amply wsp gene from Metaphycus ericeri DNA by PCR, and the product was used as templates for the following nested PCR amplification using the specific primers for A and B groups of Wolbachia. The results showed that, M.ericeri was infected by both A and B group of Wolbachia. The sequences were named as wMeeriA, wMeeriB and wMeeri, and the length were 554, 439 and 599 bp, respectively. Wolbachia in M.ericeri were classified as A-Wolbachia and Con group of B-Wolbachia by phylogenetic analysis of wsp genes. Sequences obtained from this study have been submitted to GenBank and the accession numbers assigned were HQ161162, HQ161163 and HQ161164.

Molecular Detection and Sequence Analysis of wsp Gene from Wolbachia in Metaphycus ericeri Xu et Jiang in Ericerus pela

1. Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China

Received Date: 2010-07-12

Abstract: Universal primers were used to amply wsp gene from Metaphycus ericeri DNA by PCR, and the product was used as templates for the following nested PCR amplification using the specific primers for A and B groups of Wolbachia. The results showed that, M.ericeri was infected by both A and B group of Wolbachia. The sequences were named as wMeeriA, wMeeriB and wMeeri, and the length were 554, 439 and 599 bp, respectively. Wolbachia in M.ericeri were classified as A-Wolbachia and Con group of B-Wolbachia by phylogenetic analysis of wsp genes. Sequences obtained from this study have been submitted to GenBank and the accession numbers assigned were HQ161162, HQ161163 and HQ161164.

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Reference (18)

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Molecular Detection and Sequence Analysis of wsp Gene from Wolbachia in Metaphycus ericeri Xu et Jiang in Ericerus pela

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