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Analysis of Populations Genetic Diversity of Qiongzhuea tumidinoda Using AFLP Markers

  • Received Date: 2010-05-16
  • Amplified fragment length polymorphism (AFLP)was used to analyze the genetic diversity of 2 diferent populations of Qiongzhuea tumidnoda.Ten primer pairs with higher polymorphic were selected from EcoRI/MseI primers, Total of 680 loci of the Qiongzhuea tumidnoda genome were examined for molecular variation and 662 loci were polymorphic(97.20%). PopGen32 data processing software gave out that the rate of polymorphism were 89.86% and 91.95%; the number of alleles were 1.898 6 and 1.919 5; the effective number of alleles were 1.585 0 and 1.568 3; the average genetic identities within 6 populations was 0.332 1; the average genetic identities within the 2 populations was 0.394 9; Shannon information index was 0.575 4.The genetic differentiation coefficient among the 2 populations was 0.148 8.The gene flow among the 2 populations was 2.861 0.The average genetic identity was 0.821 9 and the average genetic distance was 0.196 1.The 2 populations were divided according to the UPGMA cluster analysis.
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Analysis of Populations Genetic Diversity of Qiongzhuea tumidinoda Using AFLP Markers

  • 1. College of Forestry,Henan Agricultural University, Zhengzhou 450002, Henan, China
  • 2. Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang 311400, Zhejiang, China
  • 3.  Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang 311400, Zhejiang, China

Abstract: Amplified fragment length polymorphism (AFLP)was used to analyze the genetic diversity of 2 diferent populations of Qiongzhuea tumidnoda.Ten primer pairs with higher polymorphic were selected from EcoRI/MseI primers, Total of 680 loci of the Qiongzhuea tumidnoda genome were examined for molecular variation and 662 loci were polymorphic(97.20%). PopGen32 data processing software gave out that the rate of polymorphism were 89.86% and 91.95%; the number of alleles were 1.898 6 and 1.919 5; the effective number of alleles were 1.585 0 and 1.568 3; the average genetic identities within 6 populations was 0.332 1; the average genetic identities within the 2 populations was 0.394 9; Shannon information index was 0.575 4.The genetic differentiation coefficient among the 2 populations was 0.148 8.The gene flow among the 2 populations was 2.861 0.The average genetic identity was 0.821 9 and the average genetic distance was 0.196 1.The 2 populations were divided according to the UPGMA cluster analysis.

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