Somatic Embryogenesis and Multiplicity of Chromosome Number in Embryogenic Cell Lines of Larix ssp.

ZHANG Qing-guo; LIANG Guo-lu; HAN Su-ying; HU Na-mei; QI Li-wang

Volume 23 Issue 6

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Somatic Embryogenesis and Multiplicity of Chromosome Number in Embryogenic Cell Lines of Larix ssp.

1.
College of Horticulture and Landscape, Southwest University, Chongqing 400716, China
2.
Research Institute of Forestry, Chinese Academy of Forestry
3.
Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China

Received Date: 2009-03-19

Abstract

The embryogenesis ratios and the chromosome numbers during subculture among sixteen embryogenic cell lines derived from immature zygote of Larix spp. were compared. The results showed a negative correlation between the ability of somatic embryogenesis and the times of subculture, with embryogenesis ratios of 335.6 per gram in the 7th and 268.2 per gram in the 22nd subculture time; and a positive correlation between the embryogenesis ratios and the genetic stability, with chromosome variation ratio of 5.4% in the 7th and 40.0% in the 22nd subculture time. The ability and highly quality of somatic embryogenesis were different among cell lines, however, the most efficient embryogenic cell lines contain the most stability chromosome numbers, with embryogenesis ratio of 416.1 per gram and chromosome variation ratio of 0% in the cell line a214 after subculturing for 25times.
- Larix,
- cell lines,
- chromosome,
- somatic embryogenesis,
- genetic stability

References

[1]	Jain S, Gupta P K. Protocol for somatic embryogenesis in woody plants . Netherlands: Kluwer Academic Publishers, 2005:1-10
[2]	吕守芳,张守攻,齐力旺,等.落叶松体细胞胚胎发生研究进展[J].林业科学研究,2004,17(3):392-404
[3]	Jain S, Gupta P K, Newton R. Somatic embryogenesis in woody plant, Netherlands . Netherlands:Kluwer Academic Publishers,1995:315-339
[4]	盛德策,李凤兰,刘忠华.植物体细胞胚发生的细胞生物学研究进展[J].西北植物学报,2008,28(1):204-215
[5]	Pullman G S, Johnson S, Peter G, et al. Improving loblolly pine somatic embryo maturation:comparison of somatic and zygotic embryo morphology, germination, and gene expression[J]. Plant Cell Rep, 2003, 21:747-758
[6]	马常耕,孙晓梅.我国落叶松遗传改良现状及发展方向[J].世界林业研究,2008,21(3):58-63
[7]	张守攻,邱宏伟,韩素英,等.落叶松树种的体细胞胚胎发生与规模化技术体系[J].中国生物工程杂志,2004,24(6):28-33
[8]	张蕾.日本落叶松×华北落叶松体细胞胚胎发生的生化机制和分子机理研究 .北京:中国林业科学研究院,2008
[9]	齐力旺,李玲,韩一凡,等.落叶松不同类型胚性和非胚性愈伤组织的生理生化差异[J].林业科学,2001,37(3):20-29
[10]	齐力旺,韩一凡,李玲,等.应用311-A最优回归设计研究ABA, PEG4000及AgNO3对落叶松体细胞胚发生数量的影响[J].生物工程学报,2001,17(1):84-89
[11]	齐力旺,韩一凡,韩素英,等.芽糖、NAA及ABA对华北落叶松体细胞胚成熟及生根的影响[J].林业科学,2004,40(1):52-57
[12]	张蕾,齐力旺,韩素英. 落叶松体细胞胚原胚团阶段差异表达基因文库的构建与分析[J].分子植物育种,2008,6(4):675-682
[13]	Von Aderkas, Pattanavibool R, Hristoforoglu K,et al. Embryogenesis and genetic stability in long term megagametophytederived cultures of larch[J]. Plant Cell, Tissue and Organ Culture,2003, 75:27-34
[14]	陈瑞阳,宋文芹,李秀兰. 植物染色体标本制备的去壁、低渗法及其在细胞遗传学中的意义[J].遗传学报,1982,9(2):151-159
[15]	周键,孙建波,彭明.高等植物体细胞胚胎发生的研究进展[J].中国农学通报,2008, 24(2):129-133
[16]	Claudio S, Edward C, Yeung. Recent advances in conifer somatic embryogenesis: improving somatic embryo quality [J]. Plant Cell,Tissue and Organ Culture,2003,74:15-35
[17]	杨金玲,桂耀林,郭仲琛.白杄胚性愈伤组织长期继代培养中的分化能力及染色体稳定性研究[J].西北植物学报,2000,20(1):44-47
[18]	黄素华,赖钟雄.荔枝胚性愈伤组织及其体胚发生过程中染色体数目的变化[J].福建农林大学学报:自然科学版,2003,34(4):458-463
[19]	Wellington R C, de Carlos R C, Fernanda S A, et al. Recovering polyploid papaya in vitro regenerants as screened by flow cytometry[J]. Plant Cell Tiss Organ Cult ,2008, 92:207-214
[20]	文晓鹏,邓秀新.利用细胞学和分子标记检测刺梨愈伤组织的遗传稳定性[J].果树学报,2003,20(6):467-470
[21]	Zhang J E, Guo W W, Deng X X. Relationship Between Ploidy Variation of Citrus Calli and Competence for Somatic Embryogenesis[J]. Acta Genetica Sinica, 2006, 33 (7):647-654
[22]	陈发菊,赵志刚,梁宏伟,等. 银鹊树胚性愈伤组织继代培养过程中的细胞染色体数目变异[J].西北植物学报,2007,27(8):1600-1604
[23]	Loureiro J, Pinto G, Lopes T, et al. Assessment of ploidy stability of the somatic embryogenesis process in Quercus suber L. using flow cytometry[J]. Planta, 2005, 221:815-822
[24]	Alicja F, Jolanta M. The correlation between the chromosome variation in callus and genotype of explants of Arabidopsis thaliana[J].Genetica 2004, 121:145-154
[25]	Niskanen A M, Lu J, Seitz S, et al.. Effect of parent genotype on somatic embryogenesis in Scots pine (Pinus sylvestris) [J]. Tree Physiol,2004,24(11):1259-1265
[26]	He G Y, Zhang J R, Li K X, et al.. An improved system to establish highly embryogenic haploid cell and protoplast cultures from pollen calluses of maize (Zea mays L.) [J], Plant Cell, Tissue and Organ Culture, 2006, 86:15-25
[27]	Shim Y S, Kasha K J, Simion E, et al. The relationship between induction of embryogenesis and chromosome doubling in microspore cultures[J], Protoplasma,2006,228:79-86
[28]	Merkle SA, Montello PM, Xia X, et al. Light quality treatments enhance somatic seedling production in three southern pine species[J]. Tree Physiol,2006,26(2):187-194
[29]	Malik M R, Wang F, Dirpaul J, et al. Isolation of an embryogenic line from non-embryogenic Brassica napus cv. Westar through microspore embryogenesis[J]. Journal of Experimental Botany, 2008, 59(10):2857-2873
[30]	Hao Y J, You C X, Deng X X. Evidences for the control of chromosome number variation by a programmed-cell-death-like pathway in citrus callus Chromosome number variation in citrus callus[J]. Euphytica, 2004,140:205-212

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Somatic Embryogenesis and Multiplicity of Chromosome Number in Embryogenic Cell Lines of Larix ssp.

1. College of Horticulture and Landscape, Southwest University, Chongqing 400716, China

2. Research Institute of Forestry, Chinese Academy of Forestry

3. Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China

Received Date: 2009-03-19

Abstract: The embryogenesis ratios and the chromosome numbers during subculture among sixteen embryogenic cell lines derived from immature zygote of Larix spp. were compared. The results showed a negative correlation between the ability of somatic embryogenesis and the times of subculture, with embryogenesis ratios of 335.6 per gram in the 7th and 268.2 per gram in the 22nd subculture time; and a positive correlation between the embryogenesis ratios and the genetic stability, with chromosome variation ratio of 5.4% in the 7th and 40.0% in the 22nd subculture time. The ability and highly quality of somatic embryogenesis were different among cell lines, however, the most efficient embryogenic cell lines contain the most stability chromosome numbers, with embryogenesis ratio of 416.1 per gram and chromosome variation ratio of 0% in the cell line a214 after subculturing for 25times.

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Reference (30)

[1]	Jain S, Gupta P K. Protocol for somatic embryogenesis in woody plants . Netherlands: Kluwer Academic Publishers, 2005:1-10
[2]	吕守芳,张守攻,齐力旺,等.落叶松体细胞胚胎发生研究进展[J].林业科学研究,2004,17(3):392-404
[3]	Jain S, Gupta P K, Newton R. Somatic embryogenesis in woody plant, Netherlands . Netherlands:Kluwer Academic Publishers,1995:315-339
[4]	盛德策,李凤兰,刘忠华.植物体细胞胚发生的细胞生物学研究进展[J].西北植物学报,2008,28(1):204-215
[5]	Pullman G S, Johnson S, Peter G, et al. Improving loblolly pine somatic embryo maturation:comparison of somatic and zygotic embryo morphology, germination, and gene expression[J]. Plant Cell Rep, 2003, 21:747-758
[6]	马常耕,孙晓梅.我国落叶松遗传改良现状及发展方向[J].世界林业研究,2008,21(3):58-63
[7]	张守攻,邱宏伟,韩素英,等.落叶松树种的体细胞胚胎发生与规模化技术体系[J].中国生物工程杂志,2004,24(6):28-33
[8]	张蕾.日本落叶松×华北落叶松体细胞胚胎发生的生化机制和分子机理研究 .北京:中国林业科学研究院,2008
[9]	齐力旺,李玲,韩一凡,等.落叶松不同类型胚性和非胚性愈伤组织的生理生化差异[J].林业科学,2001,37(3):20-29
[10]	齐力旺,韩一凡,李玲,等.应用311-A最优回归设计研究ABA, PEG4000及AgNO3对落叶松体细胞胚发生数量的影响[J].生物工程学报,2001,17(1):84-89
[11]	齐力旺,韩一凡,韩素英,等.芽糖、NAA及ABA对华北落叶松体细胞胚成熟及生根的影响[J].林业科学,2004,40(1):52-57
[12]	张蕾,齐力旺,韩素英. 落叶松体细胞胚原胚团阶段差异表达基因文库的构建与分析[J].分子植物育种,2008,6(4):675-682
[13]	Von Aderkas, Pattanavibool R, Hristoforoglu K,et al. Embryogenesis and genetic stability in long term megagametophytederived cultures of larch[J]. Plant Cell, Tissue and Organ Culture,2003, 75:27-34
[14]	陈瑞阳,宋文芹,李秀兰. 植物染色体标本制备的去壁、低渗法及其在细胞遗传学中的意义[J].遗传学报,1982,9(2):151-159
[15]	周键,孙建波,彭明.高等植物体细胞胚胎发生的研究进展[J].中国农学通报,2008, 24(2):129-133
[16]	Claudio S, Edward C, Yeung. Recent advances in conifer somatic embryogenesis: improving somatic embryo quality [J]. Plant Cell,Tissue and Organ Culture,2003,74:15-35
[17]	杨金玲,桂耀林,郭仲琛.白杄胚性愈伤组织长期继代培养中的分化能力及染色体稳定性研究[J].西北植物学报,2000,20(1):44-47
[18]	黄素华,赖钟雄.荔枝胚性愈伤组织及其体胚发生过程中染色体数目的变化[J].福建农林大学学报:自然科学版,2003,34(4):458-463
[19]	Wellington R C, de Carlos R C, Fernanda S A, et al. Recovering polyploid papaya in vitro regenerants as screened by flow cytometry[J]. Plant Cell Tiss Organ Cult ,2008, 92:207-214
[20]	文晓鹏,邓秀新.利用细胞学和分子标记检测刺梨愈伤组织的遗传稳定性[J].果树学报,2003,20(6):467-470
[21]	Zhang J E, Guo W W, Deng X X. Relationship Between Ploidy Variation of Citrus Calli and Competence for Somatic Embryogenesis[J]. Acta Genetica Sinica, 2006, 33 (7):647-654
[22]	陈发菊,赵志刚,梁宏伟,等. 银鹊树胚性愈伤组织继代培养过程中的细胞染色体数目变异[J].西北植物学报,2007,27(8):1600-1604
[23]	Loureiro J, Pinto G, Lopes T, et al. Assessment of ploidy stability of the somatic embryogenesis process in Quercus suber L. using flow cytometry[J]. Planta, 2005, 221:815-822
[24]	Alicja F, Jolanta M. The correlation between the chromosome variation in callus and genotype of explants of Arabidopsis thaliana[J].Genetica 2004, 121:145-154
[25]	Niskanen A M, Lu J, Seitz S, et al.. Effect of parent genotype on somatic embryogenesis in Scots pine (Pinus sylvestris) [J]. Tree Physiol,2004,24(11):1259-1265
[26]	He G Y, Zhang J R, Li K X, et al.. An improved system to establish highly embryogenic haploid cell and protoplast cultures from pollen calluses of maize (Zea mays L.) [J], Plant Cell, Tissue and Organ Culture, 2006, 86:15-25
[27]	Shim Y S, Kasha K J, Simion E, et al. The relationship between induction of embryogenesis and chromosome doubling in microspore cultures[J], Protoplasma,2006,228:79-86
[28]	Merkle SA, Montello PM, Xia X, et al. Light quality treatments enhance somatic seedling production in three southern pine species[J]. Tree Physiol,2006,26(2):187-194
[29]	Malik M R, Wang F, Dirpaul J, et al. Isolation of an embryogenic line from non-embryogenic Brassica napus cv. Westar through microspore embryogenesis[J]. Journal of Experimental Botany, 2008, 59(10):2857-2873
[30]	Hao Y J, You C X, Deng X X. Evidences for the control of chromosome number variation by a programmed-cell-death-like pathway in citrus callus Chromosome number variation in citrus callus[J]. Euphytica, 2004,140:205-212

Somatic Embryogenesis and Multiplicity of Chromosome Number in Embryogenic Cell Lines of Larix ssp.

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通讯作者: 陈斌, bchen63@163.com

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Somatic Embryogenesis and Multiplicity of Chromosome Number in Embryogenic Cell Lines of Larix ssp.

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