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Citation:

SSRs Marking of Paeonia delavayi Based on Peony EST Data

  • Received Date: 2010-10-09
  • Through sequences analysis of Paeonia suffruticosa ESTs (expressed sequence tags) deposited in NCBI, only 335 SSRs (simple sequence repeats) were found. These SSRs are distributed in 324 ESTs out of the 2 204 ESTs examined, accounting for 14.70% of the total. The frequency of SSR was 15.20%, in which, the repetitive proportions of dinucleotide, trinucleotide, tetranucleotide and hexanucleotide were 84.14%, 15.22%, 0.30% and 0.30%, respectively. 51 candidate SSR primers were designed according to the ESTs sequences that containing SSRs, by using serafer 1.3 software. The 51 candidate SSR primers were screened against genomic DNA of 6 different flower color groups in P. delavayi, of which, 10 primer pairs amplified visible bands with polymorphism among 50 genomic DNAs from 10 color groups in P. delavayi. The results proved that the development of EST-SSR markers based on ESTs in P. suffruticosa is an effective and feasible approach, which will conduce to study on genetic diversity and genomics in P. delavayi.
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SSRs Marking of Paeonia delavayi Based on Peony EST Data

  • 1. Research Institute of Resource Insects, Chinese Academy of Forestry, Key Laboratory of Breeding and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China
  • 2. Research Institute of Forestry, Chinese Academy of Forestry , Beijing 100091, China

Abstract: Through sequences analysis of Paeonia suffruticosa ESTs (expressed sequence tags) deposited in NCBI, only 335 SSRs (simple sequence repeats) were found. These SSRs are distributed in 324 ESTs out of the 2 204 ESTs examined, accounting for 14.70% of the total. The frequency of SSR was 15.20%, in which, the repetitive proportions of dinucleotide, trinucleotide, tetranucleotide and hexanucleotide were 84.14%, 15.22%, 0.30% and 0.30%, respectively. 51 candidate SSR primers were designed according to the ESTs sequences that containing SSRs, by using serafer 1.3 software. The 51 candidate SSR primers were screened against genomic DNA of 6 different flower color groups in P. delavayi, of which, 10 primer pairs amplified visible bands with polymorphism among 50 genomic DNAs from 10 color groups in P. delavayi. The results proved that the development of EST-SSR markers based on ESTs in P. suffruticosa is an effective and feasible approach, which will conduce to study on genetic diversity and genomics in P. delavayi.

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