Detection of Quantitative Trait Loci Related with Rooting Ability of Cuttings and Growth of Eucalyptus

YU Xiao-li; LI Fa-gen; WENG Qi-jie; ZHOU Chang-pin; GAN Si-ming

Volume 24 Issue 2

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Detection of Quantitative Trait Loci Related with Rooting Ability of Cuttings and Growth of Eucalyptus

1.
Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, Guangdong, China

Received Date: 2010-07-28

Abstract

An F1 pedigree of Eucalyptus urophylla×E. tereticornis was used to detect quantitative trait loci (QTLs) controlling four cutting-related traits, i.e. number of roots per cutting, the maximum length of roots, percentage of rooted cuttings and root dry weight, as well as two growth traits, height (H) and breast-high diameter (DBH) of 13-, 18- and 46-month-old Eucalyptus trees. For cutting-related traits, six and seven QTLs were detected on genetic maps of maternal Eucalyptus urophylla and paternal E. tereticornis, respectively, with logarithm of odds (LOD) ranging from 2.0 to 3.9, variance explained from 15.2% to 26.8% and map distance with the closer flanking marker 0.0 to 15.0 cM. For growth traits, two and one QTLs were detected on the maternal map in 46-month-old H and DBH, respectively, though no QTL was found for 13- or 18-month-old growth, and a number of QTLs were located onto the paternal map for all the ages, including four, four, one, two, two and two QTLs for H13, DBH13, H18, DBH18, H46 and DBH46, respectively. No QTL was found to affect significantly the rooting ability of cuttings and field growth simultaneously, implying probably the difference in genes responsible for the two types of traits. Several markers had zero cM of map distance to or linked very closely with QTL, which might act as sound candidate markers for future marker-assisted selection in related traits.
- Eucalyptus,
- quantitative trait locus (QTL),
- rooting ability of cuttings,
- growth

References

[1]	白嘉雨. 中国热带地区桉属树种的遗传改良回顾[M]//洪菊生, 王豁然. 澳大利亚阔叶树研究. 北京: 中国林业出版社, 1993: 33-48
[2]	Clarke C R E. Are Eucalyptus clones advantageous for the pulp mill? [J]. South African Forestry Journal, 2001, 190: 61-65
[3]	Byrne M, Murrell J C, Owen J V, et al. Identification and mode of action of quantitative trait loci affecting seedling height and leaf area in Eucalyptus nitens [J]. Theoretical and Applied Genetics, 1997, 94: 674-681
[4]	Verhaegen D, Plomion C, Gion J-M, et al. Quantitative trait dissection analysis in Eucalyptus 1. Detection of QTL in interspecific hybrid progeny, stability of QTL expression across different ages [J]. Theoretical and Applied Genetics, 1997, 95: 597-608
[5]	Bundock P C, Potts B M, Vaillancourt R E. Detection and stability of quantitative trait loci (QTL) in Eucalyptus globulus[J]. Tree Genetics and Genomes, 2008, 4: 85-95
[6]	Grattagapglia D, Bertolucci F L G, Penchel R, et al. Genetic mapping of quantitative trait loci controlling growth and wood quality traits in Eucalyptus grandis using a maternal half-sib family and RAPD markers[J]. Genetics, 1996, 144: 1205-1214
[7]	Freeman J, Whittock S, Potts B, et al. QTL influencing growth and wood properties in Eucalyptus globulus[J]. Tree Genetics and Genomes, 2009, 5: 713-722
[8]	Thamarus K, Groom K, Bradley A, et al. Identification of quantitative trait loci for wood and fibre properties in two full-sib pedigrees of Eucalyptus globulus [J]. Theoretical and Applied Genetics, 2004, 109: 856-864
[9]	Thumma B R, Southerton S G, Bell J C, et al. Quantitative trait locus (QTL) analysis of wood quality traits in Eucalyptus nitens [J]. Tree Genetics and Genomes, 2010, 6: 305-317
[10]	Byrne M, Murrel J C, Owen J V, et al. Mapping of quantitative trait loci influencing frost tolerance in Eucalyptus nitens [J]. Theoretical and Applied Genetics, 1997, 95: 975-979
[11]	Henery M L, Moran G F, Wallis I R, et al. Identification of quantitative trait loci influencing foliar concentrations of terpenes and formylated phloroglucinol compounds in Eucalyptus nitens [J]. New Phytologist, 2007, 176: 82-95
[12]	Shepherd M, Chaparro J X, Teasdale R. Genetic mapping of monoterpene composition in an interspecific eucalypt hybrid [J]. Theoretical and Applied Genetics, 1999, 99: 1207-1215
[13]	Grattapaglia D, Bertolucci F, Sederoff R. Genetic mapping of QTLs controlling vegetative propagation in Eucalyptus grandis and E. urophylla using a pseudo-testcross strategy and RAPD markers [J]. Theoretical and Applied Genetics, 1995, 90: 937-947
[14]	Marques C M, Vasquez-Kool J, Carocha V J, et al. Genetic dissection of vegetative propagation traits in Eucalyptus tereticornis and E. globulus [J]. Theoretical and Applied Genetics, 1999, 99: 936-946
[15]	Marques C M, Carocha V J, de sa Pereira A R, et al. Verification of QTL linked markers for propagation traits in Eucalyptus [J]. Tree Genetics and Genomes, 2005, 1: 103-108
[16]	Gan S, Shi J, Li M, et al. Moderate-density molecular maps of Eucalyptus urophylla S. T. Blake and E. tereticornis Smith genomes based on RAPD markers [J]. Genetica, 2003, 118: 59-67
[17]	张照远, 甘四明, 李发根, 等. EST-CAPS标记在尾叶桉和细叶桉遗传图谱构建中的应用[J]. 林业科学研究, 2007, 20(2): 230-234
[18]	甘四明, 李梅, 吴坤明, 等. 尾叶桉(细叶桉杂种无性系扦插生根和生长性状的研究[J]. 林业科学研究, 2006, 19(2): 135-140
[19]	Lander E S, Botstein D. Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps [J]. Genetics, 1989, 121: 185-199
[20]	Haldane J B S. The combination of linkage values and the calculation of distance between the loci of linked factors [J]. Journal of Genetics, 1919, 8: 299-309
[21]	宋婉, 陈晓阳, 续九如, 等. 林木遗传图谱构建研究进展与发展方向[J]. 遗传, 2003, 25(6): 749- 756

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Detection of Quantitative Trait Loci Related with Rooting Ability of Cuttings and Growth of Eucalyptus

1. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, Guangdong, China

Received Date: 2010-07-28

Abstract: An F1 pedigree of Eucalyptus urophylla×E. tereticornis was used to detect quantitative trait loci (QTLs) controlling four cutting-related traits, i.e. number of roots per cutting, the maximum length of roots, percentage of rooted cuttings and root dry weight, as well as two growth traits, height (H) and breast-high diameter (DBH) of 13-, 18- and 46-month-old Eucalyptus trees. For cutting-related traits, six and seven QTLs were detected on genetic maps of maternal Eucalyptus urophylla and paternal E. tereticornis, respectively, with logarithm of odds (LOD) ranging from 2.0 to 3.9, variance explained from 15.2% to 26.8% and map distance with the closer flanking marker 0.0 to 15.0 cM. For growth traits, two and one QTLs were detected on the maternal map in 46-month-old H and DBH, respectively, though no QTL was found for 13- or 18-month-old growth, and a number of QTLs were located onto the paternal map for all the ages, including four, four, one, two, two and two QTLs for H13, DBH13, H18, DBH18, H46 and DBH46, respectively. No QTL was found to affect significantly the rooting ability of cuttings and field growth simultaneously, implying probably the difference in genes responsible for the two types of traits. Several markers had zero cM of map distance to or linked very closely with QTL, which might act as sound candidate markers for future marker-assisted selection in related traits.

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Reference (21)

[1]	白嘉雨. 中国热带地区桉属树种的遗传改良回顾[M]//洪菊生, 王豁然. 澳大利亚阔叶树研究. 北京: 中国林业出版社, 1993: 33-48
[2]	Clarke C R E. Are Eucalyptus clones advantageous for the pulp mill? [J]. South African Forestry Journal, 2001, 190: 61-65
[3]	Byrne M, Murrell J C, Owen J V, et al. Identification and mode of action of quantitative trait loci affecting seedling height and leaf area in Eucalyptus nitens [J]. Theoretical and Applied Genetics, 1997, 94: 674-681
[4]	Verhaegen D, Plomion C, Gion J-M, et al. Quantitative trait dissection analysis in Eucalyptus 1. Detection of QTL in interspecific hybrid progeny, stability of QTL expression across different ages [J]. Theoretical and Applied Genetics, 1997, 95: 597-608
[5]	Bundock P C, Potts B M, Vaillancourt R E. Detection and stability of quantitative trait loci (QTL) in Eucalyptus globulus[J]. Tree Genetics and Genomes, 2008, 4: 85-95
[6]	Grattagapglia D, Bertolucci F L G, Penchel R, et al. Genetic mapping of quantitative trait loci controlling growth and wood quality traits in Eucalyptus grandis using a maternal half-sib family and RAPD markers[J]. Genetics, 1996, 144: 1205-1214
[7]	Freeman J, Whittock S, Potts B, et al. QTL influencing growth and wood properties in Eucalyptus globulus[J]. Tree Genetics and Genomes, 2009, 5: 713-722
[8]	Thamarus K, Groom K, Bradley A, et al. Identification of quantitative trait loci for wood and fibre properties in two full-sib pedigrees of Eucalyptus globulus [J]. Theoretical and Applied Genetics, 2004, 109: 856-864
[9]	Thumma B R, Southerton S G, Bell J C, et al. Quantitative trait locus (QTL) analysis of wood quality traits in Eucalyptus nitens [J]. Tree Genetics and Genomes, 2010, 6: 305-317
[10]	Byrne M, Murrel J C, Owen J V, et al. Mapping of quantitative trait loci influencing frost tolerance in Eucalyptus nitens [J]. Theoretical and Applied Genetics, 1997, 95: 975-979
[11]	Henery M L, Moran G F, Wallis I R, et al. Identification of quantitative trait loci influencing foliar concentrations of terpenes and formylated phloroglucinol compounds in Eucalyptus nitens [J]. New Phytologist, 2007, 176: 82-95
[12]	Shepherd M, Chaparro J X, Teasdale R. Genetic mapping of monoterpene composition in an interspecific eucalypt hybrid [J]. Theoretical and Applied Genetics, 1999, 99: 1207-1215
[13]	Grattapaglia D, Bertolucci F, Sederoff R. Genetic mapping of QTLs controlling vegetative propagation in Eucalyptus grandis and E. urophylla using a pseudo-testcross strategy and RAPD markers [J]. Theoretical and Applied Genetics, 1995, 90: 937-947
[14]	Marques C M, Vasquez-Kool J, Carocha V J, et al. Genetic dissection of vegetative propagation traits in Eucalyptus tereticornis and E. globulus [J]. Theoretical and Applied Genetics, 1999, 99: 936-946
[15]	Marques C M, Carocha V J, de sa Pereira A R, et al. Verification of QTL linked markers for propagation traits in Eucalyptus [J]. Tree Genetics and Genomes, 2005, 1: 103-108
[16]	Gan S, Shi J, Li M, et al. Moderate-density molecular maps of Eucalyptus urophylla S. T. Blake and E. tereticornis Smith genomes based on RAPD markers [J]. Genetica, 2003, 118: 59-67
[17]	张照远, 甘四明, 李发根, 等. EST-CAPS标记在尾叶桉和细叶桉遗传图谱构建中的应用[J]. 林业科学研究, 2007, 20(2): 230-234
[18]	甘四明, 李梅, 吴坤明, 等. 尾叶桉(细叶桉杂种无性系扦插生根和生长性状的研究[J]. 林业科学研究, 2006, 19(2): 135-140
[19]	Lander E S, Botstein D. Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps [J]. Genetics, 1989, 121: 185-199
[20]	Haldane J B S. The combination of linkage values and the calculation of distance between the loci of linked factors [J]. Journal of Genetics, 1919, 8: 299-309
[21]	宋婉, 陈晓阳, 续九如, 等. 林木遗传图谱构建研究进展与发展方向[J]. 遗传, 2003, 25(6): 749- 756

Detection of Quantitative Trait Loci Related with Rooting Ability of Cuttings and Growth of Eucalyptus

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通讯作者: 陈斌, bchen63@163.com

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Detection of Quantitative Trait Loci Related with Rooting Ability of Cuttings and Growth of Eucalyptus

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