Characteristics of Pollen Germination in Vitro of Camellia oleifera

LI Chun-lin; YANG Shui-ping; YAO Xiao-hua; REN Hua-dong; WANG Kai-liang; LIN Ping

To study the characteristics of pollen germination in vitro of six Camellia oleifera species, the orthogonal test using six C. oleifera species as the materials was aimed to examine the effects of the 4 factors consisting of temperature, boron consumption, sucrose, and agar usage on pollen germination. The results indicated that temperature was the most significant factor among the 4 factors and the optimum temperature was 25 ℃, sucrose markedly affected the pollen germination of most species and the optimum sucrose was 10%, and boron consumption and agar usage markedly impacted on individual clones and the optimum were 100 mg·kg-1 and 0.5%. Combining multiple comparision, the optimum extractive condition is T9; the growth of pollen tube followed the "slow-fast-slow" trent. When the concentration of Ca2+ was high, the pollen germination was markedly inhibited. If the concentrations of Zn2+ and Mo6+ were low, the pollen tube growth would be enhanced.But when the concentrations of Zn2+ and Mo6+ were high, the pollen germination of pollen tube was negative effected.

1. College of Resource and Environment, Southwest University, Chongqing 400716, China

2. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China

Received Date: 2010-07-27

Abstract: To study the characteristics of pollen germination in vitro of six Camellia oleifera species, the orthogonal test using six C. oleifera species as the materials was aimed to examine the effects of the 4 factors consisting of temperature, boron consumption, sucrose, and agar usage on pollen germination. The results indicated that temperature was the most significant factor among the 4 factors and the optimum temperature was 25 ℃, sucrose markedly affected the pollen germination of most species and the optimum sucrose was 10%, and boron consumption and agar usage markedly impacted on individual clones and the optimum were 100 mg·kg-1 and 0.5%. Combining multiple comparision, the optimum extractive condition is T9; the growth of pollen tube followed the "slow-fast-slow" trent. When the concentration of Ca2+ was high, the pollen germination was markedly inhibited. If the concentrations of Zn2+ and Mo6+ were low, the pollen tube growth would be enhanced.But when the concentrations of Zn2+ and Mo6+ were high, the pollen germination of pollen tube was negative effected.

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Reference (18)

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Characteristics of Pollen Germination in Vitro of Camellia oleifera

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