Cloning and Expression Analysis of A Glycine-rich RNA-binding Protein Gene from Tamarix hispida

JIANG Bo; GAO Cai-qiu; WANG Yu-cheng; YU Li-li; YANG Chuan-ping

Volume 24 Issue 2

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Cloning and Expression Analysis of A Glycine-rich RNA-binding Protein Gene from Tamarix hispida

1.
Key Laboratory of Forest Tree Genetic improvement and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China

Received Date: 2010-07-29

Abstract

The full-length cDNA of a presumed glycine-rich ribonucleic acid (RNA) binding protein (ThGRP1) gene was isolated from a cDNA library of Tamarix hispida leaves. The ThGRP1 gene with 800 bp in length contains 432-base pair (bp) open reading frame (ORF) and encodes a 143-amino-acid polypeptide with a predicted molecular weight of 14.95 kDa and pI of 5.36. Expression of ThGRP1 in roots, stems and leaves of T. hispida with PEG, NaCl, NaHCO3 or CdCl2 treatments was studied using real-time PCR. The results showed that ThGRP1 can be induced by these stresses. Especially, the expression of ThGRP1 in roots of T. hispida was induced under all stresses at 6, 12, 24, 48 and 72 h.
- Tamarix hispida,
- ThGRP1,
- gene clone,
- real time RT-PCR

References

[1]	Kwak K J, Kim Y O, Kang H. Characterization of transgenic Arabidopsis plants overexpressing GR-RBP4 under high salinity, dehydration, or cold stress [J]. J Exp Bot, 2005, 56(421): 3007-3016
[2]	Gómez J, Sánchez-Martínez D, Stiefel V, et al. A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein [J]. Nature, 1988, 334(6179): 262-264
[3]	Van Nocker S, Vierstra R D. Two cDNAs from Arabidopsis thaliana encode putative RNA binding proteins containing glycine-rich domains[J]. Plant Molecular Biology, 1993, 21(4): 695-699
[4]	Carpenter C D, Kreps J A, Simon A E. Genes encoding glycine-rich Arabidopsis thaliana proteins with RNA-binding motifs are influenced by cold treatment and an endogenous circadian rhythm[J]. Plant Physiology, 1994, 104(3): 1015-1025
[5]	Hirose T, Sugita M, Sugiura M. cDNA structure, expression and nucleic-acid binding properties of three RNA-binding proteins in tobacco: occurrence of tissue alternative splicing[J]. Nucleic Acids Research, 1993, 21(17): 3981-3987
[6]	Moriguchi K, Sugita M, Sugiura M. Structure and subcellular localization of a small RNA-binding protein from tabacco[J]. The Plant Journal, 1997, 12(1): 215-212
[7]	Dunn M A, Brown K, Lightowlers R, et al. A lowtemperature-responsive gene from barley encodes a protein with single-stranded nucleic acid-binding activity which is phosphorylatedin vitro[J]. Plant Molecular Biology, 1996, 30(5): 947-959
[8]	Molina A, Mena M, Carbonero P, et al. Differential expression of pathogen-responsive genes encoding two types of glycine-rich proteins in barley[J]. Plant Molecular Biology, 1997, 33(5): 803-810
[9]	Bergeron D, Beauseigle D, Bellemare G. Sequence and expression of a gene encoding a protein with RNA-binding and glycine-rich domains in Brassica napus[J]. Biochimica et Biophysca Acta, 1993, 1216(1):123-125
[10]	Horvath D P, Olson P A. Cloning and characterization of coldregulated glycine-rich RNA-binding protein genes from leafy spurge (Euphorbia esula L.) and comparison to heterologous genomic clones[J]. Plant Molecular Biology, 1998, 38(4):531-538
[11]	Richard S, Drevet C, Jouanin L, et al. Isolation and characterization of a cDNA clone encoding a putative white spruce glycine-rich RNA binding protein[J]. Gene, 1999, 240(2): 379-388
[12]	Stephen J R, Dent K C, Finch-Savage W E. A cDNA encoding a cold-induced glycine-rich RNA binding protein from Prunus avium expressed in embryonic axes[J]. Gene, 2003, 320(27): 177-183
[13]	Ferullo J M, Ve'zina L P, Rail J, et al.. Differential accumulation of two glycinerich proteins during cold-acclimation alfalfa[J]. Plant Molecular Biology, 1997, 33(4): 625-633
[14]	冯缨, 尹林克. 柽柳属植物镜下器官特征描述及分类学意义[J]. 干旱区研究, 2000, 17(3): 40-45
[15]	高彩球. NaHCO₃胁迫下刚毛柽柳基因表达谱的建立及相关基因的克隆 .哈尔滨:东北林业大学,2007
[16]	王玉成, 杨传平, 姜静. 紫丁香、糖槭总RNA的快速提取方法[J]. 东北林业大学学报, 2003, 29(6): 90-91
[17]	Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method [J]. Methods, 2001, 25(4): 402-408
[18]	陈万利, 刘宗旨, 李文华. 植物富含甘氨酸蛋白质(GRP)及其基因研究进展[J]. 东北农业大学学报, 2005, 36(4): 512-519
[19]	Ringli C, Keller B, Ryser U. Glycine-richu proteins as structural components of plant cell walls[J]. Cell Mol Life Sci, 2001, 58(10): 1430-1441
[20]	Lorkovic Z J. Role of plant RNA-binding proteins in development, stress response and genome organization Trends [J]. Plant Sci, 2009, 14(4): 229-236
[21]	Kim J Y, Park S J, Jang B, et al. Functional characterization of a glycine-rich RNA-binding protein 2 in Arabidopsis thaliana under abiotic stress conditions[J]. Plant J, 2007, 50(3): 439-451
[22]	Kim J S, Jung H J, Lee H J, et al. Glycine-rich RNA-binding protein 7 affects abiotic stress responses by regulating stomata opening and closing in Arabidopsis thaliana[J]. Plant J, 2008, 55(3): 455-466
[23]	Kim Y O, Kim J S, Kang H. Cold-inducible zinc finger-containing glycine-rich RNA-binding protein contributes to the enhancement of freezing tolerance in Arabidopsis thaliana[J]. Plant J, 2005, 42(6): 890-900
[24]	Aneeta, Sanan-Mishra N, Tuteja N, et al. Salinity- and ABA-induced up-regulation and light-mediated modulation of mRNA encoding glycine-rich RNA-binding protein from Sorghum bicolor[J]. Biochem Biophys Res Commun, 2002, 296(5): 1063-1068
[25]	Nomata T, Kabeya Y, Cloning S N. characterization of glycine-rich RNA-binding protein cDNAs in the moss Physcomitrella patens[J]. Plant Cell Physiol, 2004, 45(1): 48-56
[26]	Shinozuka H, Hisano H, Yoneyama S, et al. Gene expression and genetic mapping analyses of a perennial ryegrass glycine-rich RNA-binding protein gene suggest a role in cold adaptation[J]. Mol Genet Genomics, 2006, 275(4): 399-408

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Cloning and Expression Analysis of A Glycine-rich RNA-binding Protein Gene from Tamarix hispida

1. Key Laboratory of Forest Tree Genetic improvement and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China

Received Date: 2010-07-29

Abstract: The full-length cDNA of a presumed glycine-rich ribonucleic acid (RNA) binding protein (ThGRP1) gene was isolated from a cDNA library of Tamarix hispida leaves. The ThGRP1 gene with 800 bp in length contains 432-base pair (bp) open reading frame (ORF) and encodes a 143-amino-acid polypeptide with a predicted molecular weight of 14.95 kDa and pI of 5.36. Expression of ThGRP1 in roots, stems and leaves of T. hispida with PEG, NaCl, NaHCO3 or CdCl2 treatments was studied using real-time PCR. The results showed that ThGRP1 can be induced by these stresses. Especially, the expression of ThGRP1 in roots of T. hispida was induced under all stresses at 6, 12, 24, 48 and 72 h.

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[1]	Kwak K J, Kim Y O, Kang H. Characterization of transgenic Arabidopsis plants overexpressing GR-RBP4 under high salinity, dehydration, or cold stress [J]. J Exp Bot, 2005, 56(421): 3007-3016
[2]	Gómez J, Sánchez-Martínez D, Stiefel V, et al. A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein [J]. Nature, 1988, 334(6179): 262-264
[3]	Van Nocker S, Vierstra R D. Two cDNAs from Arabidopsis thaliana encode putative RNA binding proteins containing glycine-rich domains[J]. Plant Molecular Biology, 1993, 21(4): 695-699
[4]	Carpenter C D, Kreps J A, Simon A E. Genes encoding glycine-rich Arabidopsis thaliana proteins with RNA-binding motifs are influenced by cold treatment and an endogenous circadian rhythm[J]. Plant Physiology, 1994, 104(3): 1015-1025
[5]	Hirose T, Sugita M, Sugiura M. cDNA structure, expression and nucleic-acid binding properties of three RNA-binding proteins in tobacco: occurrence of tissue alternative splicing[J]. Nucleic Acids Research, 1993, 21(17): 3981-3987
[6]	Moriguchi K, Sugita M, Sugiura M. Structure and subcellular localization of a small RNA-binding protein from tabacco[J]. The Plant Journal, 1997, 12(1): 215-212
[7]	Dunn M A, Brown K, Lightowlers R, et al. A lowtemperature-responsive gene from barley encodes a protein with single-stranded nucleic acid-binding activity which is phosphorylatedin vitro[J]. Plant Molecular Biology, 1996, 30(5): 947-959
[8]	Molina A, Mena M, Carbonero P, et al. Differential expression of pathogen-responsive genes encoding two types of glycine-rich proteins in barley[J]. Plant Molecular Biology, 1997, 33(5): 803-810
[9]	Bergeron D, Beauseigle D, Bellemare G. Sequence and expression of a gene encoding a protein with RNA-binding and glycine-rich domains in Brassica napus[J]. Biochimica et Biophysca Acta, 1993, 1216(1):123-125
[10]	Horvath D P, Olson P A. Cloning and characterization of coldregulated glycine-rich RNA-binding protein genes from leafy spurge (Euphorbia esula L.) and comparison to heterologous genomic clones[J]. Plant Molecular Biology, 1998, 38(4):531-538
[11]	Richard S, Drevet C, Jouanin L, et al. Isolation and characterization of a cDNA clone encoding a putative white spruce glycine-rich RNA binding protein[J]. Gene, 1999, 240(2): 379-388
[12]	Stephen J R, Dent K C, Finch-Savage W E. A cDNA encoding a cold-induced glycine-rich RNA binding protein from Prunus avium expressed in embryonic axes[J]. Gene, 2003, 320(27): 177-183
[13]	Ferullo J M, Ve'zina L P, Rail J, et al.. Differential accumulation of two glycinerich proteins during cold-acclimation alfalfa[J]. Plant Molecular Biology, 1997, 33(4): 625-633
[14]	冯缨, 尹林克. 柽柳属植物镜下器官特征描述及分类学意义[J]. 干旱区研究, 2000, 17(3): 40-45
[15]	高彩球. NaHCO₃胁迫下刚毛柽柳基因表达谱的建立及相关基因的克隆 .哈尔滨:东北林业大学,2007
[16]	王玉成, 杨传平, 姜静. 紫丁香、糖槭总RNA的快速提取方法[J]. 东北林业大学学报, 2003, 29(6): 90-91
[17]	Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method [J]. Methods, 2001, 25(4): 402-408
[18]	陈万利, 刘宗旨, 李文华. 植物富含甘氨酸蛋白质(GRP)及其基因研究进展[J]. 东北农业大学学报, 2005, 36(4): 512-519
[19]	Ringli C, Keller B, Ryser U. Glycine-richu proteins as structural components of plant cell walls[J]. Cell Mol Life Sci, 2001, 58(10): 1430-1441
[20]	Lorkovic Z J. Role of plant RNA-binding proteins in development, stress response and genome organization Trends [J]. Plant Sci, 2009, 14(4): 229-236
[21]	Kim J Y, Park S J, Jang B, et al. Functional characterization of a glycine-rich RNA-binding protein 2 in Arabidopsis thaliana under abiotic stress conditions[J]. Plant J, 2007, 50(3): 439-451
[22]	Kim J S, Jung H J, Lee H J, et al. Glycine-rich RNA-binding protein 7 affects abiotic stress responses by regulating stomata opening and closing in Arabidopsis thaliana[J]. Plant J, 2008, 55(3): 455-466
[23]	Kim Y O, Kim J S, Kang H. Cold-inducible zinc finger-containing glycine-rich RNA-binding protein contributes to the enhancement of freezing tolerance in Arabidopsis thaliana[J]. Plant J, 2005, 42(6): 890-900
[24]	Aneeta, Sanan-Mishra N, Tuteja N, et al. Salinity- and ABA-induced up-regulation and light-mediated modulation of mRNA encoding glycine-rich RNA-binding protein from Sorghum bicolor[J]. Biochem Biophys Res Commun, 2002, 296(5): 1063-1068
[25]	Nomata T, Kabeya Y, Cloning S N. characterization of glycine-rich RNA-binding protein cDNAs in the moss Physcomitrella patens[J]. Plant Cell Physiol, 2004, 45(1): 48-56
[26]	Shinozuka H, Hisano H, Yoneyama S, et al. Gene expression and genetic mapping analyses of a perennial ryegrass glycine-rich RNA-binding protein gene suggest a role in cold adaptation[J]. Mol Genet Genomics, 2006, 275(4): 399-408

Cloning and Expression Analysis of A Glycine-rich RNA-binding Protein Gene from Tamarix hispida

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通讯作者: 陈斌, bchen63@163.com

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Cloning and Expression Analysis of A Glycine-rich RNA-binding Protein Gene from Tamarix hispida

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