Survey and Analysis for Susceptibility of in vitro Cultured Insect Cell Lines to AcNPV and BmNPV

ZHANG Xin; FENG Ying; DING Wei-feng; MA Tao

Volume 24 Issue 3

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Article Navigation > Forest Research > 2011 > 24(3): 321-326

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Survey and Analysis for Susceptibility of in vitro Cultured Insect Cell Lines to AcNPV and BmNPV

1.
Research Institute of Resource Insects, Chinese Academy of Forestry
2.
Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2011-01-10

Abstract

Fourteen insect cell lines from three orders were used to be infected with Autographa californica nuclear polyhedrosis virus (AcNPV) and Bombyx mori nuclear polyhedrosis virus (BmNPV). The result showed that the susceptibility for two viruses were different among different insect cell lines. The cell lines which come from Diptera and Orthoptera could not be infected with AcNPV and BmNPV. The five cell lines, Sf21, Sf9, HighFive, RIRI-PX and HZ, which come from Lepidoptera, could be infected with AcNPV. The BmN cell line could be infected only with BmNPV. The infection rate of AcNPV to Sf9 was the highest, reached 84.0% after 10 days infection. RIRI-PX infected with AcNPV could produce the highest yield of polyhedral inclusion bodies which reached 43.9 PIBs/cell at 10 day post infection. The infection rate of BmNPV to BmN was 72.2% after 10 days infection, and the yield of polyhedral inclusion bodies was 23.1PIBs/cell.
- insect cell lines,
- AcNPV,
- BmNPV,
- infection

References

[1]	Maramorosch K,罗曦霞.昆虫细胞系的发展:历史回顾[J]. 武汉大学学报,1992(4):115-120
[2]	Smith G E,Summers M D. Production of human beta interferon in insect cells infected with a baculovirus expression vector[J]. Mol Cell Biol,1983, 3:2156-2165
[3]	朱江,吴祥甫. 昆虫杆状病毒表达系统研究进展及其应用展望[J].蚕业科学,2003,29 (2): 114-119
[4]	洪华珠,彭建新. 一株高水平表达重组蛋白昆虫细胞系的建立[J].昆虫学报,2001,44 (3): 276-279
[5]	Maeda S, Kawai T, Obinata M, et al. Production of human 22interferon in silkworm using a baculovirus vector [J]. Nature, 1985, 315: 592-594
[6]	常炳圣,李元.人可溶性白细胞介素-6受体基因在昆虫细胞内的克隆与表达[J]. 中国医学科学院学报,2001,23 (1): 36-37
[7]	黄亚东,郑青,李校,等.天蚕抗菌肽AD基因在AcNPV载体系统中的表达研究[J].中国抗生素杂志,2003,28(5): 304-307
[8]	吕正兵,张文平,于威,等.家蚕表达人表皮生长因子gp67信号肽融合蛋白及生物学特性研究[J].生物工程学报,2008,24(1): 111-116
[9]	王丹,BHASKAR Roy,李兴华,等. 利用杆状病毒Bac-to-Bac系统在家蚕表达猪繁殖与呼吸综合征病毒的E蛋白和M蛋白[J].蚕业科学,2010,36(5): 855-859
[10]	宋德伟,马艳,冯颖,等. 昆虫细胞工程研究进展[J]. 林业科学研究,2004 ,17(1) :116 -124
[11]	胡远扬. 昆虫病毒研究的回顾与展望[J]. 中国病毒学,2004,19 (3): 303-308
[12]	Yeh S C, Lee S T, Wu C Y, et al. A cell line (NTU-MV) established from Maruca vitrata (Lepidoptera: Pyralidae): characterization, viral susceptibility, and polyhedra production[J]. J Invertebr Pathol,2007, 96:138-146
[13]	Zhang H, Zhang Y A, Qin Q, et al. New cell lines from larval fat bodies of Spodoptera exigua: Characterization and susceptibility to baculoviruses(Lepidoptera: Noctuidae) [J]. J Invertebr Pathol, 2006, 91: 9-12
[14]	Pant U, Sudeep A B, Athawale S S, et al. Baculovirus studies in new indigenous lepidopteran cell lines[J]. Indian J Expt Biol, 2002, 40 (1):63-68
[15]	刘丽华,沈卫德. 几种核型多角体病毒感染性的检测[J].东北林业大学学报,2007,35 (10): 71-73
[16]	Wang C H, Chou C M, Liu H C, et al. Continuous cell line from pupal ovary of Perina nuda (Lepidoptera: Lymantriidae) that is permissive to nuclear polyhedrosis virus from P. nuda [J]. J Invertebr, Pathol, 1996,67:199-204
[17]	Shih C J, Lin R W, Wang C H. Establishment of a cell line from Spodoptera litura (Lepidoptera: Noctuidae) and replication of S. litura nuclear polyhedrosisvirus in vitro [J]. J Invertebr Pathol,1997,69: 1-6
[18]	Boyapalle S, Pal N, Miller W A , et al. A glassy-winged sharpshooter cell line supports replication of Rhopalosiphum padi virus (Dicistroviridae) [J].J Invertebr Pathol,2007,67:199-204
[19]	Chen Y R, Solter L F, Chien T Y, et al. Characterization of a new insect cell line (NTU-YB) derived from the common grass yellow butterfly, Eurema hecabe (Linnaeus) (Pieridae: Lepidoptera) and its susceptibility to microsporidia [J]. J Invertebr Pathol,2009,102:256-262
[20]	Levin D B, Huang J. Spodoptera littoralis Type B Nucleopolyhedrovirus Infection of a Grasshopper Cell Line [J]. J Invertebr Pathol, 1999,74;184-192
[21]	张传溪. 昆虫杆状病毒ph、pk基因分析和人EPO基因在杆状病毒-昆虫系统中的表达[M].北京:高等教育出版社,2001
[22]	吴小锋,曹翠平,许雅香, 等. BmNPV 和AcNPV 扩大寄主域杂交重组病毒表达载体的构建和改进[J]. 中国科学C辑:生命科学, 2004, 34 (2): 156-164
[23]	王文兵,马双双,李兵, 等. 利用双色荧光观察分析重组家蚕杆状病毒对昆虫细胞及蚕体组织的感染[J]. 蚕业科学,2009,35 (3) : 634-637
[24]	李充壁,庞义. 杆状病毒侵染的分子基础[J]. 微生物学通报,2001,28(5):80-84
[25]	Mori H, Nagazawa H, Shiai N, et al. Foreign gene expression by a baculovirus vector with an expanded bost rarge [J]. J Gen Virol,1992, 73:1877-1880
[26]	Argaud O, Croizier L, Lopez-Ferber M, et al. Two key mutations in the host-range specificity domain of the p143 gene of Autographa californica nucleopolyhedrovirus are required to kill Bombyx mori larvae[J]. J Gen Virol, 1998, 79 (4): 931-935
[27]	张欣,冯颖,丁伟峰,等. 七种鳞翅目昆虫细胞系染色体分析[J]. 林业科学研究, 2008,21(4):493-499
[28]	张欣,冯颖,马涛,等. 五种双翅目昆虫细胞系染色体分析[J]. 林业科学研究,2007,20(4):551-555
[29]	于洪春,王晓云,李国勋.四株昆虫细胞系对粘虫核型多角体病毒敏感性的测定[J]. 东北农业大学学报,2000,31(4):342-345
[30]	包建中,古德祥. 中国生物防治[M]. 太原:山西科学出版社,1998

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Survey and Analysis for Susceptibility of in vitro Cultured Insect Cell Lines to AcNPV and BmNPV

1. Research Institute of Resource Insects, Chinese Academy of Forestry

2. Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2011-01-10

Abstract: Fourteen insect cell lines from three orders were used to be infected with Autographa californica nuclear polyhedrosis virus (AcNPV) and Bombyx mori nuclear polyhedrosis virus (BmNPV). The result showed that the susceptibility for two viruses were different among different insect cell lines. The cell lines which come from Diptera and Orthoptera could not be infected with AcNPV and BmNPV. The five cell lines, Sf21, Sf9, HighFive, RIRI-PX and HZ, which come from Lepidoptera, could be infected with AcNPV. The BmN cell line could be infected only with BmNPV. The infection rate of AcNPV to Sf9 was the highest, reached 84.0% after 10 days infection. RIRI-PX infected with AcNPV could produce the highest yield of polyhedral inclusion bodies which reached 43.9 PIBs/cell at 10 day post infection. The infection rate of BmNPV to BmN was 72.2% after 10 days infection, and the yield of polyhedral inclusion bodies was 23.1PIBs/cell.

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Reference (30)

[1]	Maramorosch K,罗曦霞.昆虫细胞系的发展:历史回顾[J]. 武汉大学学报,1992(4):115-120
[2]	Smith G E,Summers M D. Production of human beta interferon in insect cells infected with a baculovirus expression vector[J]. Mol Cell Biol,1983, 3:2156-2165
[3]	朱江,吴祥甫. 昆虫杆状病毒表达系统研究进展及其应用展望[J].蚕业科学,2003,29 (2): 114-119
[4]	洪华珠,彭建新. 一株高水平表达重组蛋白昆虫细胞系的建立[J].昆虫学报,2001,44 (3): 276-279
[5]	Maeda S, Kawai T, Obinata M, et al. Production of human 22interferon in silkworm using a baculovirus vector [J]. Nature, 1985, 315: 592-594
[6]	常炳圣,李元.人可溶性白细胞介素-6受体基因在昆虫细胞内的克隆与表达[J]. 中国医学科学院学报,2001,23 (1): 36-37
[7]	黄亚东,郑青,李校,等.天蚕抗菌肽AD基因在AcNPV载体系统中的表达研究[J].中国抗生素杂志,2003,28(5): 304-307
[8]	吕正兵,张文平,于威,等.家蚕表达人表皮生长因子gp67信号肽融合蛋白及生物学特性研究[J].生物工程学报,2008,24(1): 111-116
[9]	王丹,BHASKAR Roy,李兴华,等. 利用杆状病毒Bac-to-Bac系统在家蚕表达猪繁殖与呼吸综合征病毒的E蛋白和M蛋白[J].蚕业科学,2010,36(5): 855-859
[10]	宋德伟,马艳,冯颖,等. 昆虫细胞工程研究进展[J]. 林业科学研究,2004 ,17(1) :116 -124
[11]	胡远扬. 昆虫病毒研究的回顾与展望[J]. 中国病毒学,2004,19 (3): 303-308
[12]	Yeh S C, Lee S T, Wu C Y, et al. A cell line (NTU-MV) established from Maruca vitrata (Lepidoptera: Pyralidae): characterization, viral susceptibility, and polyhedra production[J]. J Invertebr Pathol,2007, 96:138-146
[13]	Zhang H, Zhang Y A, Qin Q, et al. New cell lines from larval fat bodies of Spodoptera exigua: Characterization and susceptibility to baculoviruses(Lepidoptera: Noctuidae) [J]. J Invertebr Pathol, 2006, 91: 9-12
[14]	Pant U, Sudeep A B, Athawale S S, et al. Baculovirus studies in new indigenous lepidopteran cell lines[J]. Indian J Expt Biol, 2002, 40 (1):63-68
[15]	刘丽华,沈卫德. 几种核型多角体病毒感染性的检测[J].东北林业大学学报,2007,35 (10): 71-73
[16]	Wang C H, Chou C M, Liu H C, et al. Continuous cell line from pupal ovary of Perina nuda (Lepidoptera: Lymantriidae) that is permissive to nuclear polyhedrosis virus from P. nuda [J]. J Invertebr, Pathol, 1996,67:199-204
[17]	Shih C J, Lin R W, Wang C H. Establishment of a cell line from Spodoptera litura (Lepidoptera: Noctuidae) and replication of S. litura nuclear polyhedrosisvirus in vitro [J]. J Invertebr Pathol,1997,69: 1-6
[18]	Boyapalle S, Pal N, Miller W A , et al. A glassy-winged sharpshooter cell line supports replication of Rhopalosiphum padi virus (Dicistroviridae) [J].J Invertebr Pathol,2007,67:199-204
[19]	Chen Y R, Solter L F, Chien T Y, et al. Characterization of a new insect cell line (NTU-YB) derived from the common grass yellow butterfly, Eurema hecabe (Linnaeus) (Pieridae: Lepidoptera) and its susceptibility to microsporidia [J]. J Invertebr Pathol,2009,102:256-262
[20]	Levin D B, Huang J. Spodoptera littoralis Type B Nucleopolyhedrovirus Infection of a Grasshopper Cell Line [J]. J Invertebr Pathol, 1999,74;184-192
[21]	张传溪. 昆虫杆状病毒ph、pk基因分析和人EPO基因在杆状病毒-昆虫系统中的表达[M].北京:高等教育出版社,2001
[22]	吴小锋,曹翠平,许雅香, 等. BmNPV 和AcNPV 扩大寄主域杂交重组病毒表达载体的构建和改进[J]. 中国科学C辑:生命科学, 2004, 34 (2): 156-164
[23]	王文兵,马双双,李兵, 等. 利用双色荧光观察分析重组家蚕杆状病毒对昆虫细胞及蚕体组织的感染[J]. 蚕业科学,2009,35 (3) : 634-637
[24]	李充壁,庞义. 杆状病毒侵染的分子基础[J]. 微生物学通报,2001,28(5):80-84
[25]	Mori H, Nagazawa H, Shiai N, et al. Foreign gene expression by a baculovirus vector with an expanded bost rarge [J]. J Gen Virol,1992, 73:1877-1880
[26]	Argaud O, Croizier L, Lopez-Ferber M, et al. Two key mutations in the host-range specificity domain of the p143 gene of Autographa californica nucleopolyhedrovirus are required to kill Bombyx mori larvae[J]. J Gen Virol, 1998, 79 (4): 931-935
[27]	张欣,冯颖,丁伟峰,等. 七种鳞翅目昆虫细胞系染色体分析[J]. 林业科学研究, 2008,21(4):493-499
[28]	张欣,冯颖,马涛,等. 五种双翅目昆虫细胞系染色体分析[J]. 林业科学研究,2007,20(4):551-555
[29]	于洪春,王晓云,李国勋.四株昆虫细胞系对粘虫核型多角体病毒敏感性的测定[J]. 东北农业大学学报,2000,31(4):342-345
[30]	包建中,古德祥. 中国生物防治[M]. 太原:山西科学出版社,1998

Survey and Analysis for Susceptibility of in vitro Cultured Insect Cell Lines to AcNPV and BmNPV

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Survey and Analysis for Susceptibility of in vitro Cultured Insect Cell Lines to AcNPV and BmNPV

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