Molecular Detection and Sequence Analysis of wsp Gene from Wolbachia in a New Species of Ericerus pela Parasitic Wasps

YANG Pu; ZHU Jia-ying; XIE Zheng-hua; LI Meng; CHEN Xiao-ming; CHEN Xiao-qing

PCR amplification was employed to detect the infection of Wolbachia on Aprostocetus sp., a new species of parasitic wasps on Ericerus pela, by using the universal primers and specific primers for wsp gene of A and B supergroup. The sequences obtained, were named as wApr, wAprA and wAprB, which sizes were 620, 566 and 463 bp respectively. Sequence analysis found that 4 variable basepairs occurred among the sequences. The phylogenetic analysis of wsp gene showed that Wolbachia in Aprostocetus sp. were classified as Dig group of B-Wolbachia. The sequences of wApr and wAprB were submitted to GenBank and the accession numbers assigned were HQ121415 and HQ121417.

1. Research Institute of Resource Insects, Chinese Academy of Forestry

2. The Key laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2010-11-25

Abstract: PCR amplification was employed to detect the infection of Wolbachia on Aprostocetus sp., a new species of parasitic wasps on Ericerus pela, by using the universal primers and specific primers for wsp gene of A and B supergroup. The sequences obtained, were named as wApr, wAprA and wAprB, which sizes were 620, 566 and 463 bp respectively. Sequence analysis found that 4 variable basepairs occurred among the sequences. The phylogenetic analysis of wsp gene showed that Wolbachia in Aprostocetus sp. were classified as Dig group of B-Wolbachia. The sequences of wApr and wAprB were submitted to GenBank and the accession numbers assigned were HQ121415 and HQ121417.

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Reference (22)

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Molecular Detection and Sequence Analysis of wsp Gene from Wolbachia in a New Species of Ericerus pela Parasitic Wasps

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