• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

Citation:

Effects of Chlorpyrifos on Food Utilization and Detoxifying Enzymes and Acetylcholinesterase of Lymantria dispar

  • Received Date: 2010-01-07
  • The effects of sublethal dose of chlorpyrifos (CPF) on food utilization of gypsy moth, Lymantria dispar, as well as CPF toxicity and enzyme activities of carboxylesterase (CarE), glutathione S-transferases (GSTs) and acetylcholinesterase (AChE) were evaluated in the 3rd-instar gypsy moth larvae using gravimetric method and measuring enzyme activities. Relative growth rate (RGR), efficiency of the conversion of ingested food (ECI) and efficiency of the conversion of digested food (ECD) of 3rd-instar larvae fed on poplar leaves treated by sublethal concentration of CPF were significantly lower than those fed on normal polar leaves. However, approximate digestibility (AD) of the treatment was significantly higher than that of control. The relative consumption rate (RCR) of the treatment and control groups were not significantly different. The 24 h LC50 of CPF to the gypsy moth 3rd-instar larvae was 5.86 mg·L-1, which was higher than that of omethoate but lower than that of cyhalothrin. In vitro inhibition assay indicated that the activities of AChE, CarE and GSTs were inhibited by CPF depended on concentrations. The enzyme activity inhibition percent is CarE>AChE>GSTs. The results suggest CPF is a kind of effective organophosphorous insecticide for controlling gypsy moth.
  • 加载中
  • [1] 胡春祥.舞毒蛾生物防治研究进展[J].东北林业大学学报,2002,30 (4):40-43

    [2] 王 香,陈晓萍,方 舒,等. 毒死蜱对神经系统发育毒性作用的研究进展[J]. 生物学杂志,2009,26 (3): 56-58

    [3] 朱文达,郭嗣斌,涂爱萍,等. 48%毒死蜱乳油对水稻稻纵卷叶螟的防治效果[J]. 华中农业大学学报,2008,27(1):56-58

    [4] 卢 颖,姜 龙,毛军旗,等. 48%毒死蜱EC防治水稻潜叶蝇的药效试验[J]. 农药,2008,47 (4):300-301

    [5] 郑丽祯,傅建炜,陈小龙,等. 黄曲条跳甲对毒死蜱敏感性差异的生化机制[J]. 昆虫知识,2009,46(2):256-259

    [6]

    Waldbauer G P. The consumption, digestion and utilization of solanaceous and non-solanaceous plants by larvae of the tobacco hornworm, Protoparce sexta (Johan.) (Lepidoptera:Sphingidae)[J]. Entomologia Experimentalis et Applicata, 1964, 7:253
    [7]

    Van Aspern K. A study of housefly esterase by means of a sensitive colorimetric method [J]. Insect Physiology, 1962, 8: 401-406
    [8] 高希武,郑炳宗,梁同庭,等. 杀虫剂混用或加增效剂对爪-棉蚜增效作用及机制的研究[J]. 植物保护学报,1989,16 (4):273-278

    [9] 高希武. Gorun等改进的Ellman胆碱酯酶活性测定方法介绍[J].昆虫知识,1987,24(4):245-246

    [10] 刘洪霞,高希武,冷培恩. 花翅摇蚊乙酰胆碱酯酶的底物专一性及对胆碱酯酶抑制剂敏感度的比较[J]. 应用与环境生物学报,2008,14(2):211-214

    [11]

    Habig W H, Pabst M J, Jakoby W B. Glutathione S-transferase AA from rat liver[J]. Archives of Biochemistry and Biophysics,1976,175(2):710-716
    [12]

    Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding [J]. Analytical Biochemistry, 1976, 72 (2):248-254
    [13]

    Fountain M T, Browna V K, Gange AC, et al. The effects of the insecticide chlorpyrifos on spider and Colembola communities [J]. Pedobiologia, 2007, 51:147-158
    [14] 唐文成,孙虹霞,舒迎花,等. 低浓度毒死蜱对斜纹夜蛾幼虫生长发育和食物利用的影响[J]. 环境昆虫学报,2009,31(2): 137-142

    [15] 高海燕,王 静,朱九生,等. 亚致死剂量甲氨基阿维菌素苯甲酸盐对家蚕食物利用的影响[J]. 农药学学报,2008,10 (3):297-302

    [16]

    Rath S S, Prasad B C, Sinha BRRP. Food utilization efficency in fifth instar larvae of Antheraes mylitta (Lepidop tera: Saturniidae) infected with Nosema sp. and its effects on reproductive potential and silk p roduction[J]. Journal of Invertebrate Pathology, 2003, 83:1-9
    [17] 唐振华,毕 强. 杀虫剂作用的分子行为 . 上海:上海远东出版社,2003:58-110

    [18]

    Cao C W, Zhang J, Gao X W, et al. Overexpression of carboxylesterase gene associated with organophosphorous insecticide resistance in cotton aphids, Aphis gossypii (Glover)[J]. Pesticide biochemistry and physiology, 2008, 90: 175-180
    [19]

    Wheelock C E, Shan G, Ottea J. Overview of carboxylesterases and their role in the metabolism of insecticides [J]. Journal of Pesticide Science, 2005, 30:75-83
    [20]

    Motoyama N, Dauterman W C. Glutathione S-transferases: Their role in the metabolism of organophosphorus insecticides[J]. Review of Biochemistry and Toxicology, 1980, 2:49-69
    [21]

    Yu S J.Consequences of induction of foreign compounds metabolizing-enzyme in insects[M]//Brattsten L B, Ahmad S. Molecular Aspects of Insect-Plant Association. Plenum Press, New York,1986:153
    [22] 杨正先,孟范平,刘 强,等. 家蝇AChE检测毒死蜱残留的最佳条件研究[J]. 环境科学与技术,2006,29(7):42-44

  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article views(3339) PDF downloads(1779) Cited by()

Proportional views

Effects of Chlorpyrifos on Food Utilization and Detoxifying Enzymes and Acetylcholinesterase of Lymantria dispar

  • 1. Department of Forestry Protection, Northeast Forestry University, Harbin 150040, Heilongjiang, China
  • 2. Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University), Ministry of Education, Harbin 150040, Heilongjiang, C

Abstract: The effects of sublethal dose of chlorpyrifos (CPF) on food utilization of gypsy moth, Lymantria dispar, as well as CPF toxicity and enzyme activities of carboxylesterase (CarE), glutathione S-transferases (GSTs) and acetylcholinesterase (AChE) were evaluated in the 3rd-instar gypsy moth larvae using gravimetric method and measuring enzyme activities. Relative growth rate (RGR), efficiency of the conversion of ingested food (ECI) and efficiency of the conversion of digested food (ECD) of 3rd-instar larvae fed on poplar leaves treated by sublethal concentration of CPF were significantly lower than those fed on normal polar leaves. However, approximate digestibility (AD) of the treatment was significantly higher than that of control. The relative consumption rate (RCR) of the treatment and control groups were not significantly different. The 24 h LC50 of CPF to the gypsy moth 3rd-instar larvae was 5.86 mg·L-1, which was higher than that of omethoate but lower than that of cyhalothrin. In vitro inhibition assay indicated that the activities of AChE, CarE and GSTs were inhibited by CPF depended on concentrations. The enzyme activity inhibition percent is CarE>AChE>GSTs. The results suggest CPF is a kind of effective organophosphorous insecticide for controlling gypsy moth.

Reference (22)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return