cDNA Cloning and Sequence Analysis of β1-Tubulin from Chinese White Wax Scale Ericerus pela Chavannes (Homopetera: Coccidae)

YANG Pu; CHEN Xiao-ming; ZHU Jia-ying; DING Wei-feng; LIU Wei-wei

Volume 24 Issue 4

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cDNA Cloning and Sequence Analysis of β1-Tubulin from Chinese White Wax Scale Ericerus pela Chavannes (Homopetera: Coccidae)

1.
Research Institute of Resource Insects, Chinese Academy of Forestry, The Key laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China
2.
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Faculty of Conservation Biology, Southwest Forestry University, Kunming 650224, Yunnan, China

Received Date: 2011-01-29

Abstract

A full-length cDNA of β1-tubulin gene from Ericerus pela was obtained by using RACE and RT-PCR methods. The size of β1-tubulin gene is 1492 bp, which contains an open reading frame of 1 344 bp that encoding 447 amino acid residues with 50.20 kda molecular weight and 4.75 isoelectric point. The putative amino acid sequences contain conserved sequences of β1-tubulin and the GTP-binding site. The phylogenetic analysis showed that β1-tubulin gene of E. pela shares high identity with β-tubulin homologues from other insects. The sequences of β1-tubulin gene of E. pela were submitted to GenBank and the accession number was assigned JF731244.
- Ericerus pela,
- tubulin,
- RACE,
- amino acid

References

[1]	樊东, 秦松柏, 朴冬花, 等. 二化螟β1-微管蛋白基因cDNA序列的克隆与序列分析[J]. 生物技术通报, 2008, 34(4): 130-135
[2]	于奎峰, 李红亮, 杨璞, 等. 稻水象甲卵巢内β1-微管蛋白基因cDNA全长的克隆及其定量表达分析[J]. 中国水稻科学, 2010, 24(6): 575-580
[3]	Yang P, Zhou W W, Zhang Q, et al. Differential gene expression profiling in the developed ovaries between the parthenogenetic and bisexual female rice water weevils, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae)[J]. Chinese Science Bulletin, 2009, 54: 3822-3829
[4]	Li M, Yang Q, Song J Z. Three tubulin genes of Trichoderma harzianum: alpha, beta and gamma[J]. Brazilian Archives of Biology and Technology, 2010, 53(4): 811-816
[5]	Hetland T E, Hellesylt E, Flrenes V A, et al. Class Ⅲ β-tubulin expression in advanced-stage serous ovarian carcinoma effusions is associated with poor survival and primary chemoresistance[J]. Human Pathology, 2011, (待发表)
[6]	Jensen-Smith H C, Eley J, Steyger P S, et al. Cell type-specific reduction of beta tubulin isotypes synthesized in the developing gerbil organ of Corti[J]. Journal of Neurocytology, 2003, 32(2): 185-197
[7]	Woo K, Jensen-Smith H C, Luduena R F, et al. Differential synthesis of beta-tubulin isotypes in gerbil nasal epithelia[J]. Cell and Tissue Research, 2002, 309(2): 331-335
[8]	Kirschner M, Mitchison T. Beyond self-assembly: from microtubules to morphogenesis[J]. Cell, 1986, 45: 329-342
[9]	Yen T J, Gay D A, Pachter J S, et al. Autoregulated changes in stability of polyribosome-bound beta-tubulin mRNAs are specified by the first 13 translated nucleotides[J]. Molecular and Cellular Biology. 1988, 8(3): 1224-1235
[10]	Yen T J, Machlin P S, Cleveland D W. Autoregulated instability of beta-tubulin mRNAs by recognition of the nascent amino terminus of beta-tubulin[J]. Nature, 1988, 334(6183): 580-585
[11]	Cleveland D W. Autoregulated control of tubulin synthesis in animal cells[J]. Current Opinion in Cell Biology, 1989, 1(1): 10-14
[12]	陈晓鸣, 冯颖. 资源昆虫学概论[M]. 北京: 科学出版社, 2009: 29-39
[13]	Luduena R F. Multiple forms of tubulin: different gene products and covalent modifications[J]. International Review of Cytology, 1998, 178: 207-275
[14]	Carpenter E J, Huzil J T, Luduena R F, et al. Homology modeling of tubulin: influence predictions for microtubule’s biophysical properties[J]. European Biophysics Journal, 2006, 36(1): 35-43
[15]	Michiels F, Falkenburg D, Müller A M, et al. Testis-specific beta 2 tubulins are identical in Drosophila melanogaster and D. hydei but differ from the ubiquitous beta 1 tubulin[J]. Chromosoma, 1987, 95(6): 387-395
[16]	Rudolph J E, Kimble M, Hoyle H D, et al. Three Drosophila beta-tubulin sequences: a developmentally regulated isoform (beta 3), the testis-specific isoform (beta 2), and an assembly-defective mutation of the testis-specific isoform (B2t8) reveal both an ancient divergence in metazoan isotypes and structural constraints for beta-tubulin function[J]. Molecular Cell Biology, 1987, 7(6): 2231-2242
[17]	Goldstein L S B, Gunawardena S. Flying through the Drosophila cytoskeletal genome[J]. Journal of Cell Biology, 2000, 150(2): F63-F68
[18]	Kawasaki H, Sugaya K, Guo X Q, et al. Analysis of α-and β-tubulin genes of Bombyx mori using an EST database[J]. Insect Biochemistry and Molecular Biology, 2003, 33(1): 131-137
[19]	Mita K, Nenoi M, Morimyo M, et al. Bombyx mori beta-tubulin gene specifically expressed in testis[J]. Gene, 1995, 162, 329-330
[20]	Quan G.X, Kanke E, Kawasaki H. Isolation and particular expression of a new β-tubulin gene in wing discs during metamorphosis of Bombyx mori[J]. Journal of Sericultural Science of Japan, 1998, 67(1): 43-50
[21]	Hachouf-Gheras S, Besson MT, Bosquet G. Identification and developmental expression of a Bombyx mori alpha-tubulin gene[J]. Gene, 1998, 208(1): 89-94
[22]	Raff E C, Fackenthal J D, Hutchens J A, et al. Microtubule architecture specified by a beta-tubulin isoform[J]. Science, 1997, 275(5296): 70-73
[23]	Rybczynski R, Gilbert L I. Cloning of a beta1 tubulin cDNA from an insect endocrine gland: developmental and hormone-induced changes in mRNA expression[J]. Molecular and Cellular Endocrinology, 1998, 141: 141-151

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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cDNA Cloning and Sequence Analysis of β1-Tubulin from Chinese White Wax Scale Ericerus pela Chavannes (Homopetera: Coccidae)

1. Research Institute of Resource Insects, Chinese Academy of Forestry, The Key laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China

2. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Faculty of Conservation Biology, Southwest Forestry University, Kunming 650224, Yunnan, China

Received Date: 2011-01-29

Abstract: A full-length cDNA of β1-tubulin gene from Ericerus pela was obtained by using RACE and RT-PCR methods. The size of β1-tubulin gene is 1492 bp, which contains an open reading frame of 1 344 bp that encoding 447 amino acid residues with 50.20 kda molecular weight and 4.75 isoelectric point. The putative amino acid sequences contain conserved sequences of β1-tubulin and the GTP-binding site. The phylogenetic analysis showed that β1-tubulin gene of E. pela shares high identity with β-tubulin homologues from other insects. The sequences of β1-tubulin gene of E. pela were submitted to GenBank and the accession number was assigned JF731244.

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Reference (23)

[1]	樊东, 秦松柏, 朴冬花, 等. 二化螟β1-微管蛋白基因cDNA序列的克隆与序列分析[J]. 生物技术通报, 2008, 34(4): 130-135
[2]	于奎峰, 李红亮, 杨璞, 等. 稻水象甲卵巢内β1-微管蛋白基因cDNA全长的克隆及其定量表达分析[J]. 中国水稻科学, 2010, 24(6): 575-580
[3]	Yang P, Zhou W W, Zhang Q, et al. Differential gene expression profiling in the developed ovaries between the parthenogenetic and bisexual female rice water weevils, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae)[J]. Chinese Science Bulletin, 2009, 54: 3822-3829
[4]	Li M, Yang Q, Song J Z. Three tubulin genes of Trichoderma harzianum: alpha, beta and gamma[J]. Brazilian Archives of Biology and Technology, 2010, 53(4): 811-816
[5]	Hetland T E, Hellesylt E, Flrenes V A, et al. Class Ⅲ β-tubulin expression in advanced-stage serous ovarian carcinoma effusions is associated with poor survival and primary chemoresistance[J]. Human Pathology, 2011, (待发表)
[6]	Jensen-Smith H C, Eley J, Steyger P S, et al. Cell type-specific reduction of beta tubulin isotypes synthesized in the developing gerbil organ of Corti[J]. Journal of Neurocytology, 2003, 32(2): 185-197
[7]	Woo K, Jensen-Smith H C, Luduena R F, et al. Differential synthesis of beta-tubulin isotypes in gerbil nasal epithelia[J]. Cell and Tissue Research, 2002, 309(2): 331-335
[8]	Kirschner M, Mitchison T. Beyond self-assembly: from microtubules to morphogenesis[J]. Cell, 1986, 45: 329-342
[9]	Yen T J, Gay D A, Pachter J S, et al. Autoregulated changes in stability of polyribosome-bound beta-tubulin mRNAs are specified by the first 13 translated nucleotides[J]. Molecular and Cellular Biology. 1988, 8(3): 1224-1235
[10]	Yen T J, Machlin P S, Cleveland D W. Autoregulated instability of beta-tubulin mRNAs by recognition of the nascent amino terminus of beta-tubulin[J]. Nature, 1988, 334(6183): 580-585
[11]	Cleveland D W. Autoregulated control of tubulin synthesis in animal cells[J]. Current Opinion in Cell Biology, 1989, 1(1): 10-14
[12]	陈晓鸣, 冯颖. 资源昆虫学概论[M]. 北京: 科学出版社, 2009: 29-39
[13]	Luduena R F. Multiple forms of tubulin: different gene products and covalent modifications[J]. International Review of Cytology, 1998, 178: 207-275
[14]	Carpenter E J, Huzil J T, Luduena R F, et al. Homology modeling of tubulin: influence predictions for microtubule’s biophysical properties[J]. European Biophysics Journal, 2006, 36(1): 35-43
[15]	Michiels F, Falkenburg D, Müller A M, et al. Testis-specific beta 2 tubulins are identical in Drosophila melanogaster and D. hydei but differ from the ubiquitous beta 1 tubulin[J]. Chromosoma, 1987, 95(6): 387-395
[16]	Rudolph J E, Kimble M, Hoyle H D, et al. Three Drosophila beta-tubulin sequences: a developmentally regulated isoform (beta 3), the testis-specific isoform (beta 2), and an assembly-defective mutation of the testis-specific isoform (B2t8) reveal both an ancient divergence in metazoan isotypes and structural constraints for beta-tubulin function[J]. Molecular Cell Biology, 1987, 7(6): 2231-2242
[17]	Goldstein L S B, Gunawardena S. Flying through the Drosophila cytoskeletal genome[J]. Journal of Cell Biology, 2000, 150(2): F63-F68
[18]	Kawasaki H, Sugaya K, Guo X Q, et al. Analysis of α-and β-tubulin genes of Bombyx mori using an EST database[J]. Insect Biochemistry and Molecular Biology, 2003, 33(1): 131-137
[19]	Mita K, Nenoi M, Morimyo M, et al. Bombyx mori beta-tubulin gene specifically expressed in testis[J]. Gene, 1995, 162, 329-330
[20]	Quan G.X, Kanke E, Kawasaki H. Isolation and particular expression of a new β-tubulin gene in wing discs during metamorphosis of Bombyx mori[J]. Journal of Sericultural Science of Japan, 1998, 67(1): 43-50
[21]	Hachouf-Gheras S, Besson MT, Bosquet G. Identification and developmental expression of a Bombyx mori alpha-tubulin gene[J]. Gene, 1998, 208(1): 89-94
[22]	Raff E C, Fackenthal J D, Hutchens J A, et al. Microtubule architecture specified by a beta-tubulin isoform[J]. Science, 1997, 275(5296): 70-73
[23]	Rybczynski R, Gilbert L I. Cloning of a beta1 tubulin cDNA from an insect endocrine gland: developmental and hormone-induced changes in mRNA expression[J]. Molecular and Cellular Endocrinology, 1998, 141: 141-151

cDNA Cloning and Sequence Analysis of β1-Tubulin from Chinese White Wax Scale Ericerus pela Chavannes (Homopetera: Coccidae)

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通讯作者: 陈斌, bchen63@163.com

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cDNA Cloning and Sequence Analysis of β1-Tubulin from Chinese White Wax Scale Ericerus pela Chavannes (Homopetera: Coccidae)

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