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Citation:

The Molecular Identification and Phylogeny of Tephrosia Witches’-broom Phytoplasma

  • Received Date: 2010-12-06
  • The tephrosia witches’-broom phytoplasma collected from Hainan province was classified and identified by sequencing techniques and iPhyClassifer. The results of sequencing and homologuous comparison with other phytoplasmas showed that tephrosia witches’-broom phytoplasma shared 99.9% similarity with peanut witches’-broom phytoplasma in 16S rRNA gene and 99.9% in rp gene. Tephrosia witches’-broom phytoplasma are sorted into Peanut witches’-broom group(16SrⅡ), 16SrⅡ-A, according to the results of iPhyClassifer of 16S rDNA, phylogenetic analysis of 16S rRNA and rp gene, which is related to Ca. Phytoplasma australasiae.
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  • [1]

    Seemüller E, Marcone C, Lauer U, et al. Current status of molecular classification of the phytoplasmas[J]. Journal of Plant Pathology, 1998, 80: 3-26
    [2] 李 永, 徐启聪, 田国忠,等. 猪屎豆丛枝病植原体的分子检测与鉴定[J]. 林业科学,2010, 46(1):163-168

    [3] 赖 帆,李 永,徐启聪,等. 植原体的最新分类研究动态[J]. 微生物学通报, 2008, 35(2):291-295

    [4]

    Zhao Y, Wei W, Lee I M, et al. Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII) [J]. Int J Syst Evol Microbiol, 2009, 59: 2582-2593
    [5]

    Lee I M, Hammond R W, Davis R E, et al. Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms[J]. Phytopathology, 1993b,83: 834-842
    [6]

    Lee I M, Davis R E, Dawn E, et al. Phytoplasma: phytopathogenic mollicutes[J]. Annu Rev Microbiol, 2000, 54 :221-253
    [7]

    The IRPCM Phytoplasma/Spiroplasma Working Team-Phytoplasma taxonomy group. 'Candidatus Phytoplasma’ a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects[J]. Int J Syst Evol Microbiol, 2004, 54: 1243-1255
    [8]

    Wei W, Davis R E, Lee I M, et al. Computer-simulated RFLP analysis of 16S rRNA genes: identification of ten new phytoplasma groups[J]. Int J Syst Evol Microbiol, 2007, 57:1855-1867
    [9]

    Martini M, Lee I M, Bottner D, et al. Ribosomal protein gene-based phylogeny for finer differentiation and classification of phytoplasmas[J]. Int J Syst Evol Microbiol, 2007, 57: 2037-2051
    [10]

    Deng S, Hiruki C. Amplification of 16S rRNA genes from culturable and nonculturable Mollicutes[J]. J Microbiol Methods, 1991, 14:53-61
    [11] 温秀军,郭晓军,田国忠,等. 几个枣树品种和婆枣单株对枣疯病抗性的鉴定[J]. 林业科学,2005, 41(3):88-96

    [12] 王海妮,吴云锋,安凤秋,等. 枣疯病和酸枣丛枝病植原体16S rDNA 和tuf基因的序列同源性分析[J]. 中国农业科学, 2007, 40(10):2200-2205

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    沈阳化工大学材料科学与工程学院 沈阳 110142

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The Molecular Identification and Phylogeny of Tephrosia Witches’-broom Phytoplasma

  • 1. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry
  • 2. Key Laboratory of Forest Protection, State Forestry Administration, Beijing 100091, China

Abstract: The tephrosia witches’-broom phytoplasma collected from Hainan province was classified and identified by sequencing techniques and iPhyClassifer. The results of sequencing and homologuous comparison with other phytoplasmas showed that tephrosia witches’-broom phytoplasma shared 99.9% similarity with peanut witches’-broom phytoplasma in 16S rRNA gene and 99.9% in rp gene. Tephrosia witches’-broom phytoplasma are sorted into Peanut witches’-broom group(16SrⅡ), 16SrⅡ-A, according to the results of iPhyClassifer of 16S rDNA, phylogenetic analysis of 16S rRNA and rp gene, which is related to Ca. Phytoplasma australasiae.

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