• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

Citation:

Physiological and Biochemical Response of Dodonaea viscose Seeds to Desiccation

  • Received Date: 2011-03-25
  • Ultra-dry storage of Dodonaea viscose seeds was studied by orthogonal design including four factors (seed moisture content, packing method, storage temperature and pre-humidification method). Seeds were dried to the moisture contents ranging from 1.12% to 6.13% with silica gel, then stored in hermetic condition combined with different treatments for a year. The germination percentage, electrical conductivity (RC), proline (Pro), malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), total soluble sugar content and fatty acid composition of the stored seeds were tested. The results showed that the seed moisture content had significant effect on germination percentage. The feasible moisture content ranged from 4.28% to 5.25%.The Germination percentage had a significantly negative correlation with the electrical conductivity and proline content, and had significantly positive correlation with the proline content and activity of three antioxidant enzymes. The content of oleic acid and α-Linolenic acid in ultra-dried seeds were higher compared to the control seeds. It is speculated that the seed deterioration of D. viscose during storage was the result of membrane injury and lipid peroxidation. Suitable ultra-dried treatment, especially in seed moisture content, can increase the activity of antioxidant enzyme and the content of unsaturated fatty acid, which alleviate the seed deterioration.
  • 加载中
  • [1]

    Buitink J, Leprince O. Glass formation in plant anhydrobiotes: survival in the dry state[J]. Cryobiology, 2004, 48(3):215-228
    [2]

    Sankaranarayanan S, Bama P, Ramachandran J, et al. Ethnobotanical study of medicinal plants used by traditional users in Villupuram district of Tamil Nadu, India[J]. Journal of Medicinal Plants Research, 2010, 4(12):1089-1101
    [3]

    Abulude F O. Phytochemical Screnning and Mineral Contents of Leaves of Some Nigerian Woody Plants[J]. Research Journal of Phytochemistry, 2007, 1(1):33-39
    [4]

    Berjak P, Pammenter N. From Avicennia to Zizania: seed recalcitrance in perspective[J]. Annals of Botany, 2008, 101(2):213-228
    [5] 宋松泉,程红炎,龙春林,等. 种子生物学研究指南[M]. 北京: 科学出版社, 2005

    [6] 国际种子检验协会(ISTA). 1996国际种子检验规程. (农业部全国农作物种子质量监督检测中心,浙江大学种子科学中心译)[M]. 北京:中国农业出版社, 1996

    [7] 洪 伟. 林业试验设计技术与方法[M]. 北京: 科学技术出版社, 1993

    [8] 李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2003

    [9]

    Slade L, Levine H. Water and the glass transition-Dependence of the glass transition on composition and chemical structure: Special implications for flour functionality in cookie baking[J]. Journal of Food Engineering, 1995, 24(4):431-509
    [10]

    Chang B S, Beauvais R M, Dong A, et al. Physical factors affecting the storage stability of freeze-dried interleukin-1 receptor antagonist: glass transition and protein conformation[J]. Archives of Biochemistry and Biophysics, 1996, 331(2):249-258
    [11]

    Prestrelski S J, Tedeschi N, Arakawa T, et al. Dehydration-induced conformational transitions in proteins and their inhibition by stabilizers[J]. Biophysical Journal, 1993, 65(2):661-671
    [12]

    Ellis R H, Hong T D. Temperature sensitivity of the low-moisture-content limit to negative seed longevity moisture content relationships in hermetic storage[J]. Annals of botany, 2006, 97(5):785-791
    [13]

    Zheng G H. Ultradry seed storage: Improve strategy and technology for germplasm conservation[J]. Chin Biodiv, 1998(2):61-65
    [14]

    Leprince O, Vertucci C W, Hendry G A F, et al. The expression of desiccation-induced damage in orthodox seeds is a function of oxygen and temperature[J]. Physiologia Plantarum, 1995, 94(2):233-240
    [15]

    Li Y, Feng H Y, Chen T, et al. Physiological responses of Limonium aureum seeds to ultra-drying[J]. Journal of Integrative Plant Biology, 2007, 49(5):569-575
    [16]

    Li Y, Qu J J, Dong Z B, et al. Storage behavior of Zygophyllum xanthoxylon (Bge.) Maxim seeds at low moisture contents[J]. Acta Physiologiae Plantarum, 2008, 30(5):651-656
    [17]

    Mira S, Estrelles E, González-Benito M E, et al. Biochemical changes induced in seeds of Brassicaceae wild species during ageing[J]. Acta Physiologiae Plantarum, 2011:1-7
    [18]

    Noctor G, Foyer C H. Ascorbate and glutathione: keeping active oxygen under control[J]. Annual Review of Plant Biology, 1998, 49(1):249-279
    [19]

    Goel A, Sheoran I S. Lipid peroxidation and peroxide-scavenging enzymes in cotton seeds under natural ageing[J]. Biologia Plantarum, 2003, 46(3):429-434
    [20]

    Song S Q, Berjak P, Pammenter N. Desiccation sensitivity of Trichilia dregeana axes and antioxidant role of ascorbic acid[J]. Acta Botanica Sinica, 2004, 46(7):803-810
    [21]

    Zhu C, Chen J. Changes in soluble sugar and antioxidant enzymes in peanut seeds during ultra dry storage and after accelerated aging[J]. Seed Science and Technology, 2007, 35(2):387-401
    [22]

    Horbowicz M, Obendorf R L. Seed desiccation tolerance and storability: dependence on flatulence-producing oligosaccharides and cyclitols-review and survey[J]. Seed Science Research, 1994, 4(4):385-405
    [23]

    Leprince O, Walters-Vertucci C. A calorimetric study of the glass transition behaviors in axes of bean seeds with relevance to storage stability[J]. Plant Physiology, 1995, 109(4):1471-1481
    [24]

    Wolkers W F, Tetteroo F A A, Alberda M, et al. Changed properties of the cytoplasmic matrix associated with desiccation tolerance of dried carrot somatic embryos. An in situ Fourier transform infrared spectroscopic study[J]. Plant Physiology, 1999, 120(1):153-164
    [25]

    van den Dries I J, van Dusschoten D, Hemminga M A. Mobility in maltose-water glasses studied with 1H NMR[J]. The Journal of Physical Chemistry B, 1998, 102(51):10483-10489
    [26]

    MacFarlane D, Pringle J, Annat G. Reversible self-polymerizing high Tg lyoprotectants[J]. Cryobiology, 2002, 45(2):188-192
    [27]

    Buitink J, Leprince O. Intracellular glasses and seed survival in the dry state[J]. Comptes Rendus Biologies, 2008, 331(10):788-795
    [28]

    Weber H. Fatty acid-derived signals in plants[J]. Trends in Plant Science, 2002, 7(5):217-224
    [29]

    Rao R G S, Singh P M, Rai M. Storability of onion seeds and effects of packaging and storage conditions on viability and vigour[J]. Scientia Horticulturae, 2006, 110(1):1-6
    [30] 胡小荣,陶 梅,卢新雄,等.不同含水量大葱种子贮藏后的脂肪代谢研究[J].植物遗传资源学报,2006,7(4):464-467

    [31] 崔 凯,李 昆,李 立,等.木豆种子超干保存最适含水量的选择及其机制分析[J].东北林业大学学报,2008, 36(5):19-21

    [32] 崔 凯,李 昆.酸角种子超干保存最适含水量的选择及机制分析[J]. 西北农业学报,2008,17(3):186-190

  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article views(2995) PDF downloads(1486) Cited by()

Proportional views

Physiological and Biochemical Response of Dodonaea viscose Seeds to Desiccation

  • 1. Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China
  • 2. Yuanmou Desert Ecosystem Research Station of the State Forestry Administration, Kunming 650224, Yunnan, China

Abstract: Ultra-dry storage of Dodonaea viscose seeds was studied by orthogonal design including four factors (seed moisture content, packing method, storage temperature and pre-humidification method). Seeds were dried to the moisture contents ranging from 1.12% to 6.13% with silica gel, then stored in hermetic condition combined with different treatments for a year. The germination percentage, electrical conductivity (RC), proline (Pro), malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), total soluble sugar content and fatty acid composition of the stored seeds were tested. The results showed that the seed moisture content had significant effect on germination percentage. The feasible moisture content ranged from 4.28% to 5.25%.The Germination percentage had a significantly negative correlation with the electrical conductivity and proline content, and had significantly positive correlation with the proline content and activity of three antioxidant enzymes. The content of oleic acid and α-Linolenic acid in ultra-dried seeds were higher compared to the control seeds. It is speculated that the seed deterioration of D. viscose during storage was the result of membrane injury and lipid peroxidation. Suitable ultra-dried treatment, especially in seed moisture content, can increase the activity of antioxidant enzyme and the content of unsaturated fatty acid, which alleviate the seed deterioration.

Reference (32)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return