Cloning of Tung Tree DGAT2 Gene and Construction of RNAi Binary Expression Vector with Convergent Promoters

XU Ling-na; WANG Yang-dong; CHEN Yi-cun; ZHANG Shan-shan

To identify the physiological function of Diacylglycerol Acyltransferase 2 (DGAT2) coding gene in the process of tung oil biosynthesis, DGAT2 was cloned from cDNA of tung tree kernel and then linked with pMD18-T vector for sequencing. The 969bp fragment containing Open Reading Frame was acquired. Subsequently, RNAi binary expression vector pD35-DGAT2 was constructed, which expressed DGAT2 in two opposite ways. The studies provide the possibilities to further identify the function of DGAT2 in tung oil biosynthesis by RNAi technology and hold promise for genetic engineering of Venicia fordii.

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China

2. College of Life Science, Anhui Normal University, Wuhu 241000, Anhui, China

Received Date: 2010-11-26

Abstract: To identify the physiological function of Diacylglycerol Acyltransferase 2 (DGAT2) coding gene in the process of tung oil biosynthesis, DGAT2 was cloned from cDNA of tung tree kernel and then linked with pMD18-T vector for sequencing. The 969bp fragment containing Open Reading Frame was acquired. Subsequently, RNAi binary expression vector pD35-DGAT2 was constructed, which expressed DGAT2 in two opposite ways. The studies provide the possibilities to further identify the function of DGAT2 in tung oil biosynthesis by RNAi technology and hold promise for genetic engineering of Venicia fordii.

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Cloning of Tung Tree DGAT2 Gene and Construction of RNAi Binary Expression Vector with Convergent Promoters

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通讯作者: 陈斌, bchen63@163.com

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