Development of Polymorphic Simple Sequence Repeats Markers in Phyllostachys edulis by Magnesphere

PENG Zhen-hua; LIU Guan-shui; LI Lu-bin

The objective of this work is to seek SSR markers for Phyllostachys edulis. Magnesphere method was used to conentrate SSR containing sequences from Ph. edulis Genomic DNA AFLP fragments. Three SSR-enriched libraries (GT, AG, and CCA) were constructed. The Clone-PCR method was used to screen positive clones, 1 080, 620, 630 clones were screened in GT, AG, CCA libraries, and 137, 73, 41 SSR-containing sequences were obtained, at concentration rate of 12.7%, 11.8%, 6.5% respectively. The result showed that the concentration rate of dinucleotide repeat libraries is higher than trinucleotide libraries. After sequences analyzing, 53 pairs of SSR primers were designed, 31 of which amplified the objective fragment in Ph. edulis at the rate of 58.9%.

1. International Centre for Bamboo and Rattan, Beijing 100102, China

2. Research Institute of Forestry, Chinese Academy of Forestry

Received Date: 2010-06-30

Available Online: 2011-12-13

Abstract: The objective of this work is to seek SSR markers for Phyllostachys edulis. Magnesphere method was used to conentrate SSR containing sequences from Ph. edulis Genomic DNA AFLP fragments. Three SSR-enriched libraries (GT, AG, and CCA) were constructed. The Clone-PCR method was used to screen positive clones, 1 080, 620, 630 clones were screened in GT, AG, CCA libraries, and 137, 73, 41 SSR-containing sequences were obtained, at concentration rate of 12.7%, 11.8%, 6.5% respectively. The result showed that the concentration rate of dinucleotide repeat libraries is higher than trinucleotide libraries. After sequences analyzing, 53 pairs of SSR primers were designed, 31 of which amplified the objective fragment in Ph. edulis at the rate of 58.9%.

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Development of Polymorphic Simple Sequence Repeats Markers in Phyllostachys edulis by Magnesphere

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