Simultaneous Determination of Paclitaxel in Hazelnut by HPLC-MS/MS

LUO Fan; FEI Xue-qian; TANG Fu-bin; LI Xin-lei

Established method by reverse phase HPLC and electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis and hazelnut extract trace of taxol. Through the research, seven mass spectrum characteristics of taxol compounds were contested, and the taxanes database was initially established. It was found that the paciltaxel contents were 23.65 μg·mL-1 and 4.09 μg·mL-1 in leaves and stems respectively. The paciltaxel contents in different parts of hazelnut was slightly different, which were 0.61, 0.23 and 0.35 μg·mL-1 in hazel branch, leaf and leather. With these data it can quickly identify the known paclitaxel compounds in hazelnut extract, and can perform the unknown structure analysis to certain degrees. This analysis method is confirmed sensitive, reliable and fast.

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China

Received Date: 2011-03-21

Available Online: 2011-12-13

Abstract: Established method by reverse phase HPLC and electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis and hazelnut extract trace of taxol. Through the research, seven mass spectrum characteristics of taxol compounds were contested, and the taxanes database was initially established. It was found that the paciltaxel contents were 23.65 μg·mL-1 and 4.09 μg·mL-1 in leaves and stems respectively. The paciltaxel contents in different parts of hazelnut was slightly different, which were 0.61, 0.23 and 0.35 μg·mL-1 in hazel branch, leaf and leather. With these data it can quickly identify the known paclitaxel compounds in hazelnut extract, and can perform the unknown structure analysis to certain degrees. This analysis method is confirmed sensitive, reliable and fast.

HTML

Reference (21)

[1]	雷雨田. 晚期癌症克星--紫杉醇传奇[J]. 医药世界, 2008(7):42-43
[2]	唐朝晖,钟德玝. 紫杉醇抗肿瘤的分子机制[J]. 中国临床康复,2006,10(27):125-127
[3]	王立坤,李宝英,边丽. 1例罕见紫杉醇诱发严重痛风反应患者的护理[J]. 中国实用护理杂志:上旬版,2007, 23 (6):57
[4]	白荣芬,张利萍. 紫杉组培繁育试验初报[J]. 林业实用技术,2007(1):19-20
[5]	周志春,余能健. 栽培措施对南方红豆杉紫杉醇含量的影响[J]. 林业科学研究, 2010,23 (1):120-124
[6]	谢谊,许慧敏,王实强. HPLC法测定不同部位南方红豆杉中紫杉烷类化合物的含量[J]. 湖南中医药大学学报,2009,29(6):26-28
[7]	李秋云,李炜,崔洋. 高效液相色谱法测定曼地亚红豆杉中紫杉醇的含量[J]. 陕西中医学院学报,2009,32(5):64-65
[8]	郭娜,周志强,王洋. 东北红豆杉中紫杉醇及三尖杉宁碱含量测定方法的研究[J]. 林业科技,2009(3):62-64
[9]	张洁,段继诚,梁振,等. 东北红豆杉及其伤愈组织粗提物中紫杉醇的HPLC-ESI-MS/MS分析研究[J]. 药学学报,2006,41(9):863-866
[10]	梅兴国,鲁明波.紫杉醇的抗癌作用及治疗其他疾病的潜力[J]. 国外医学:药学分册,1996,23(3):136-140
[11]	Wani M C, Taylor H L, Wall M E, et al. Plant antitumor agents VI. The Isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia[J]. Am Chem Soc,1971,93(9):2325-2327
[12]	邱德有,吴小红,黄璐琦. 木本药用植物红豆杉研究的新进展[J]. 林业科学研究,2009,22(3):439-445
[13]	李永成,陶文沂. 美丽镰刀菌培养液对东北红豆杉悬浮细胞防御反应及紫杉醇合成的影响[J]. 西北植物学报,2008,28(9):1746-1750
[14]	严冰,毕建男,纪元,等. 一株柏树内生真菌产生抗肿瘤药物紫杉醇[J]. 南开大学学报:自然科学版,2007, 40(6):67-70
[15]	王世伟,马玺,平文祥,等. 微生物发酵生产紫杉醇研究进展[J]. 微生物学通报,2007,34(3):561-565
[16]	赵广河,张培正,李大鹏. RP-HPLC法测定榛叶中紫杉醇的研究[J]. 山东农业大学学报:自然科学版,2009,40(3):385-388
[17]	潘东海,谷会岩,孙慧珍. 毛榛资源利用与开发前景[J]. 中国林副特产,1999(1):41
[18]	丛金山. 榛子栽培技术[J]. 河北林业科技,2010(4):108
[19]	王昌伟,彭少麟,李鸣光. 红豆杉中紫杉醇及其衍生物含量影响因子研究进展[J]. 生态学报,2006,26 (5):1583-1590
[20]	高山林,朱丹妮,周荣汗. 东亚和北美产红豆杉属七种植物中紫杉醇及短叶醇的含量[J]. 中国药科大学学报, 1995,26 (1):8
[21]	Witherup K M,Look S A,Stasko M W. Taxus spp. Needles contain amount of taxol comparable to the bark of Taxus brevifobia: analysis and isolation [J]. J Nat Prod, 1990, 53 (5) : 1249-1255

Simultaneous Determination of Paclitaxel in Hazelnut by HPLC-MS/MS

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views