Cryopreservation of Cell Line L-2/M delta 2-3 from Embryo of Drosophila melanogaster:The Effects of Cell Density on the Cytoactive

DING Wen-feng; FENG Ying; ZHANG Xin; LI Xian

In order to evaluate the effects of different cell density on post-thaw cytoactives ( including cell viability, recovery time, etc.) of insect cell line L-2/M delta 2-3 from embryo of Drosophila melanogaster Fallen, 10 test groups with different cell density (from 0.81×106 cells·mL-1 to 2.88×107 cells·mL-1, the density span of each group was more than 2.00×106 cells·mL-1 ) were prepared as the cryopreserved cultures preserved in liquid nitrogen separately during 14 months. The results showed that the post-thaw viability of each test group decreased with the cryopreservation time expended. The rate of post-thaw viability decline decreased with the cell density of cryopreserved cultures increased. Cultures frozen at above 1.3×107 cells·mL-1 could obtain a better effect of cryopreservation.

1. Research Institute of Resource Insects, Chinese Academy of Forestry

Received Date: 2011-09-12

Available Online: 2012-02-16

Abstract: In order to evaluate the effects of different cell density on post-thaw cytoactives ( including cell viability, recovery time, etc.) of insect cell line L-2/M delta 2-3 from embryo of Drosophila melanogaster Fallen, 10 test groups with different cell density (from 0.81×106 cells·mL-1 to 2.88×107 cells·mL-1, the density span of each group was more than 2.00×106 cells·mL-1 ) were prepared as the cryopreserved cultures preserved in liquid nitrogen separately during 14 months. The results showed that the post-thaw viability of each test group decreased with the cryopreservation time expended. The rate of post-thaw viability decline decreased with the cell density of cryopreserved cultures increased. Cultures frozen at above 1.3×107 cells·mL-1 could obtain a better effect of cryopreservation.

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Reference (13)

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Cryopreservation of Cell Line L-2/M delta 2-3 from Embryo of Drosophila melanogaster:The Effects of Cell Density on the Cytoactive

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