Relationship between Growth Traits Heterosis and Genetic Distance among Parents of Pinus elliottii×P.caribaea Based on SSR Molecular Markers

LI Yi-liang; ZHAO Fen-cheng; WU Hui-shan; ZHANG Ying-zhong; LI Fu-ming; ZHONG Sui-ying; LI Xian-zheng; CAI Jian

Volume 25 Issue 2

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Relationship between Growth Traits Heterosis and Genetic Distance among Parents of Pinus elliottii×P.caribaea Based on SSR Molecular Markers

1.
Guangdong Academy of Forestry, Guangzhou 510520, Guangdong, China
2.
Taishan Hongling Seed Orchard, Taishan 529200, Guangdong, China

Received Date: 2010-11-30

Abstract

Seventeen Pinus elliottii×P. caribaea parental lines were assayed by Simple Sequence Repeat (SSR) to evaluate the genetic diversity and determine the correlation between genetic distance and heterosis. The results indicated that there were abundant genetic polymorphisms at 14 SSR molecular markers in parents of hybrid pine, 57 alleles were detected in total, the percentage of polymorphic loci was 87.72%,and the average heterozygosity ranged between 0.144 4 and 0.884 1, the microsatellite loci could be used for genetic diversity evaluation in parents of hybrid pine. The results showed that there was significant relation or extremely significant relation between genetic distance and height, DBH and volume of heterosis. The correlative coefficients were 0.542 0, 0.641 2 and 0.639 3, and the determination coefficient were 0.293 8, 0.411 0 and 0.408 7. Since there existed a relative closer correlation between the genetic distance and height, DBH and volume of heterosis, it is feasible to predict the hybrid pines heterosis by SSR markers.
- Pinus elliottii×P.caribaea,
- SSR markers,
- genetic distance,
- heterosis

References

[1]	赵奋成, 李宪政, 张应中, 等. 湿地松与洪都拉斯加勒比松的杂交效果分析[J]. 林业科学研究, 2006, 19 (4) : 409-415
[2]	Dudley J W, Saghai Maroof M A, Rufener G K. Molecular marker and grouping of parents in maize breeding programs [J]. Crop Science, 1991, 31: 718-723
[3]	袁力行, 傅骏骅, 刘新芝, 等. 利用分子标记预测玉米杂种优势的研究[J].中国农业科学, 2000, 33(6): 6-12
[4]	张涛, 倪先林, 蒋开锋, 等. 水稻功能基因标记遗传距离与杂种优势的相关性研究[J].中国水稻科学, 2009, 23(6): 567-572
[5]	张礼霞, 王林友, 张利华, 等. 用Rim2超级家族分子指纹鉴别杂交水稻及预测杂种优势[J]. 作物学报, 2007, 33(1): 77-83
[6]	沈金雄, 傅廷栋, 杨光圣. 甘蓝型油菜 SSR、ISSR 标记的遗传多样性及其与杂种表现的关系[J]. 中国农业科学, 2004, 37(4): 477-483
[7]	高洪斌, 司龙亭, 宋铁峰, 等. 黄瓜亲本遗传距离与F₁产量优势相关关系的研究[J]. 辽宁农业科学, 2002 (3): 9-12
[8]	武耀廷, 张天真, 朱协飞, 等. 陆地棉遗传距离与杂种F₁、F₂产量及杂种优势的相关分析[J]. 中国农业科学, 2002, 35(1): 22-28
[9]	詹克慧, 孙洪, 高翔, 等. 小麦亲本间分子遗传距离与F₁杂种优势的相关性分析[J]. 麦类作物学报, 2006, 26(2): 27-31
[10]	张一, 储德裕, 金国庆, 等. 马尾松亲本遗传距离与子代生长性状相关性分析[J]. 林业科学研究, 2010, 23(2): 215-220
[11]	李善文, 张志毅, 于志水, 等. 杨树杂交亲本分子遗传距离与子代生长性状的相关性[J]. 林业科学, 2008, 44(5): 150-154
[12]	李义良, 赵奋成, 张应中, 等. 适用于微卫星标记的湿地松、加勒比松DNA快速提取法[J]. 生物技术通报, 2010 (1): 83-86
[13]	Devey M E, Sewell M M, Uren T L, et al.Comparative mapping in loblolly and radiata pine using RFLP and microsatellite markers[J]. Theoretical and Applied Genetics, 1999, 99: 656-662
[14]	Fisher P J, Richardson T E, Gardner R C. Characteristics of single and mulucopy microsatellite from Pinus radiata[J]. Theoretical and Applied Genetics, 1998, 96: 969-979
[15]	Baril C P, Verhaegen D, Vigneron P, et al. Structure of the specific combining ability between two species of Eucalyptus. I. RAPD data [J]. Theoretical and Applied Genetics, 1997, 94:796-803
[16]	李周岐, 王章荣. 用RAPD标记进行鹅掌楸杂种识别和亲本选配[J]. 林业科学, 2002, 38(5):169-174
[17]	Reif J C, Melchinger A E, Xia X C, et al. Use of SSRs for establishing heterotic groups in subtropical maize [J]. Theoretical and Applied Genetics, 2003, 107(5): 947-957
[18]	Chowdari K V, Venkatachalam S R, Davierwala A P, et al. Hybrid performance and genetic distance as revealed by the (GATA) 4 microsatellite and RAPD markers in pearl millet [J]. Theoretical and Applied Genetics, 1997, 97: 163-169
[19]	王晓阳. 利用SSR分子标记探测鹅掌楸杂种优势. 南京:南京林业大学, 2009
[20]	Lanza L L B, Souza C L, Ottoboni L M M, et al. Genetic distance of inbred lines and prediction of maize single-cross performance using RAPD markers[J]. Theoretical and Applied Genetics, 1997, 94: 1023-1030
[21]	Riday H, Brummer C, Campbell T A, et al. Comparisons of genetic and morphological distance with heterosis between Medicago sativa subsp. sativa and subsp. falcate[J]. Euphytica, 2003, 131: 37-45
[22]	Boppenmaier J, Melchinger A E, Sertz G. Genetic diversity for RFLPs in European maize inbreed performance of crosses within between heterosis group for grain traits[J]. Plant Breeding, 1993, 113: 219-226
[23]	赵庆勇, 朱镇, 张亚东, 等. SSR标记遗传距离与粳稻杂种优势的相关性分析[J]. 中国水稻科学, 2009, 23(2): 141-147
[24]	谭祖猛, 李云昌, 胡琼, 等. 通过分子标记估算遗传距离预测甘蓝型油菜的杂种优势[J]. 中国油料作物学报, 2007, 29(2):126-132
[25]	倪先林, 张涛, 蒋开锋,等. SSR分子标记与水稻杂种优势的相关性研究[J]. 安徽农业科学, 2009, 37(23): 10913-10916
[26]	Smith O S, Smith J S C, Bowen S L, et al. Similarities among a group of elite maize in breeds as measured by pedigree,F_lgrain yield,grain yield heterosis and RFLPs[J]. Theoretical and Applied Genetics, 1990, 80: 833-840
[27]	张培江, 才宏伟, 李焕朝, 等. RAPD分子标记水稻遗传距离及其与杂种优势的关系[J]. 安徽农业科学, 2000, 28(6): 697-700,704

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Relationship between Growth Traits Heterosis and Genetic Distance among Parents of Pinus elliottii×P.caribaea Based on SSR Molecular Markers

1. Guangdong Academy of Forestry, Guangzhou 510520, Guangdong, China

2. Taishan Hongling Seed Orchard, Taishan 529200, Guangdong, China

Received Date: 2010-11-30

Available Online: 2012-04-13

Abstract: Seventeen Pinus elliottii×P. caribaea parental lines were assayed by Simple Sequence Repeat (SSR) to evaluate the genetic diversity and determine the correlation between genetic distance and heterosis. The results indicated that there were abundant genetic polymorphisms at 14 SSR molecular markers in parents of hybrid pine, 57 alleles were detected in total, the percentage of polymorphic loci was 87.72%,and the average heterozygosity ranged between 0.144 4 and 0.884 1, the microsatellite loci could be used for genetic diversity evaluation in parents of hybrid pine. The results showed that there was significant relation or extremely significant relation between genetic distance and height, DBH and volume of heterosis. The correlative coefficients were 0.542 0, 0.641 2 and 0.639 3, and the determination coefficient were 0.293 8, 0.411 0 and 0.408 7. Since there existed a relative closer correlation between the genetic distance and height, DBH and volume of heterosis, it is feasible to predict the hybrid pines heterosis by SSR markers.

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Reference (27)

[1]	赵奋成, 李宪政, 张应中, 等. 湿地松与洪都拉斯加勒比松的杂交效果分析[J]. 林业科学研究, 2006, 19 (4) : 409-415
[2]	Dudley J W, Saghai Maroof M A, Rufener G K. Molecular marker and grouping of parents in maize breeding programs [J]. Crop Science, 1991, 31: 718-723
[3]	袁力行, 傅骏骅, 刘新芝, 等. 利用分子标记预测玉米杂种优势的研究[J].中国农业科学, 2000, 33(6): 6-12
[4]	张涛, 倪先林, 蒋开锋, 等. 水稻功能基因标记遗传距离与杂种优势的相关性研究[J].中国水稻科学, 2009, 23(6): 567-572
[5]	张礼霞, 王林友, 张利华, 等. 用Rim2超级家族分子指纹鉴别杂交水稻及预测杂种优势[J]. 作物学报, 2007, 33(1): 77-83
[6]	沈金雄, 傅廷栋, 杨光圣. 甘蓝型油菜 SSR、ISSR 标记的遗传多样性及其与杂种表现的关系[J]. 中国农业科学, 2004, 37(4): 477-483
[7]	高洪斌, 司龙亭, 宋铁峰, 等. 黄瓜亲本遗传距离与F₁产量优势相关关系的研究[J]. 辽宁农业科学, 2002 (3): 9-12
[8]	武耀廷, 张天真, 朱协飞, 等. 陆地棉遗传距离与杂种F₁、F₂产量及杂种优势的相关分析[J]. 中国农业科学, 2002, 35(1): 22-28
[9]	詹克慧, 孙洪, 高翔, 等. 小麦亲本间分子遗传距离与F₁杂种优势的相关性分析[J]. 麦类作物学报, 2006, 26(2): 27-31
[10]	张一, 储德裕, 金国庆, 等. 马尾松亲本遗传距离与子代生长性状相关性分析[J]. 林业科学研究, 2010, 23(2): 215-220
[11]	李善文, 张志毅, 于志水, 等. 杨树杂交亲本分子遗传距离与子代生长性状的相关性[J]. 林业科学, 2008, 44(5): 150-154
[12]	李义良, 赵奋成, 张应中, 等. 适用于微卫星标记的湿地松、加勒比松DNA快速提取法[J]. 生物技术通报, 2010 (1): 83-86
[13]	Devey M E, Sewell M M, Uren T L, et al.Comparative mapping in loblolly and radiata pine using RFLP and microsatellite markers[J]. Theoretical and Applied Genetics, 1999, 99: 656-662
[14]	Fisher P J, Richardson T E, Gardner R C. Characteristics of single and mulucopy microsatellite from Pinus radiata[J]. Theoretical and Applied Genetics, 1998, 96: 969-979
[15]	Baril C P, Verhaegen D, Vigneron P, et al. Structure of the specific combining ability between two species of Eucalyptus. I. RAPD data [J]. Theoretical and Applied Genetics, 1997, 94:796-803
[16]	李周岐, 王章荣. 用RAPD标记进行鹅掌楸杂种识别和亲本选配[J]. 林业科学, 2002, 38(5):169-174
[17]	Reif J C, Melchinger A E, Xia X C, et al. Use of SSRs for establishing heterotic groups in subtropical maize [J]. Theoretical and Applied Genetics, 2003, 107(5): 947-957
[18]	Chowdari K V, Venkatachalam S R, Davierwala A P, et al. Hybrid performance and genetic distance as revealed by the (GATA) 4 microsatellite and RAPD markers in pearl millet [J]. Theoretical and Applied Genetics, 1997, 97: 163-169
[19]	王晓阳. 利用SSR分子标记探测鹅掌楸杂种优势. 南京:南京林业大学, 2009
[20]	Lanza L L B, Souza C L, Ottoboni L M M, et al. Genetic distance of inbred lines and prediction of maize single-cross performance using RAPD markers[J]. Theoretical and Applied Genetics, 1997, 94: 1023-1030
[21]	Riday H, Brummer C, Campbell T A, et al. Comparisons of genetic and morphological distance with heterosis between Medicago sativa subsp. sativa and subsp. falcate[J]. Euphytica, 2003, 131: 37-45
[22]	Boppenmaier J, Melchinger A E, Sertz G. Genetic diversity for RFLPs in European maize inbreed performance of crosses within between heterosis group for grain traits[J]. Plant Breeding, 1993, 113: 219-226
[23]	赵庆勇, 朱镇, 张亚东, 等. SSR标记遗传距离与粳稻杂种优势的相关性分析[J]. 中国水稻科学, 2009, 23(2): 141-147
[24]	谭祖猛, 李云昌, 胡琼, 等. 通过分子标记估算遗传距离预测甘蓝型油菜的杂种优势[J]. 中国油料作物学报, 2007, 29(2):126-132
[25]	倪先林, 张涛, 蒋开锋,等. SSR分子标记与水稻杂种优势的相关性研究[J]. 安徽农业科学, 2009, 37(23): 10913-10916
[26]	Smith O S, Smith J S C, Bowen S L, et al. Similarities among a group of elite maize in breeds as measured by pedigree,F_lgrain yield,grain yield heterosis and RFLPs[J]. Theoretical and Applied Genetics, 1990, 80: 833-840
[27]	张培江, 才宏伟, 李焕朝, 等. RAPD分子标记水稻遗传距离及其与杂种优势的关系[J]. 安徽农业科学, 2000, 28(6): 697-700,704

Relationship between Growth Traits Heterosis and Genetic Distance among Parents of Pinus elliottii×P.caribaea Based on SSR Molecular Markers

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通讯作者: 陈斌, bchen63@163.com

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Relationship between Growth Traits Heterosis and Genetic Distance among Parents of Pinus elliottii×P.caribaea Based on SSR Molecular Markers

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