Cloning and Expression Analysis of ChWRKY2 from Corylus heterophylla  under Low Temperature Stress

ZHAO Tian-tian; WANG Gui-xi; LIANG Li-song; MA Qing-hua; CHEN Xin

According to the high-throughput sequencing results of hazelnut buds, a 675 bp fragment, ChWRKY2, which encoded a polypeptide of 179 amino acids was obtained through RACE-PCR.The deduced protein sequence shows that this protein belongs to the second group of WRKY family and the zinc-finger structure is C-X4-C-X23-H-X1-H.The ChWRKY2 expression in hazelnut buds was analyzed under natural conditions and the result displayed that the expression level was the highest in December and then decreased. ChWRKY2 in leaves were rapidly and highly induced when suckers were exposed to low temperatures of 4 ℃,ChWRKY2 were activated at 8 h reaching a peak. the spatial expression analysis revealed that the transcription level of ChWRKY2 was relatively higher in male inflorescence than that in bud and bark tissues.

Cloning and Expression Analysis of ChWRKY2 from Corylus heterophylla under Low Temperature Stress

1. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2011-12-27

Available Online: 2012-04-13

Abstract: According to the high-throughput sequencing results of hazelnut buds, a 675 bp fragment, ChWRKY2, which encoded a polypeptide of 179 amino acids was obtained through RACE-PCR.The deduced protein sequence shows that this protein belongs to the second group of WRKY family and the zinc-finger structure is C-X4-C-X23-H-X1-H.The ChWRKY2 expression in hazelnut buds was analyzed under natural conditions and the result displayed that the expression level was the highest in December and then decreased. ChWRKY2 in leaves were rapidly and highly induced when suckers were exposed to low temperatures of 4 ℃,ChWRKY2 were activated at 8 h reaching a peak. the spatial expression analysis revealed that the transcription level of ChWRKY2 was relatively higher in male inflorescence than that in bud and bark tissues.

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Reference (16)

[1]	Ulker B, Somssich I E. WRKY transcription factors:from DNA binding towards biological function [J].Current Opinion in Plant Biology, 2004,7(5): 491-498
[2]	Ishiguro S, Nakamura K. Characterization of a cDNA encoding a novel DNA-binding protein, SPF1, that recognizes SP8 sequences in the 50 upstream regions of genes coding for sporamin and beta-amylase from sweet potato,Mol[J]. Gen Genet, 1994, 244(6): 563-571
[3]	Chujo T, Takai R, Akimoto T. Involvement of the elicitor induced gene OsWRKY53 in the expression of defense related genes in rice [J].Biochemica et Biophysica Acta,2007,1769(7-8):497-505
[4]	李冉, 娄永根. 植物中逆境反应相关的WRKY转录因子研究进展[J].生态学报, 2011, 31(11):3223-3231
[5]	李秀霞, 牛成功, 邵红, 等.佳木斯地区榛子引种试验初报[J].中国野生植物资源, 2005, 24(6):72-74
[6]	吕跃东, 董凤祥, 王贵禧, 等.平欧杂交榛抗寒性综合评价体系的建立与应用[J].林业科学, 2008, 44(9):31-35
[7]	Rinne P, Welling A, Kaikuranta P. Onset of freezing tolerance in birch (Betula pubescens Ehrh.) involves LEA proteins and osmoregulation and is impaired in an ABA-deficient genotype[J].Plant Cell and Environment, 1998,21(6): 601-611
[8]	陈新.榛子花芽转录组文库的Solexa测序及冷调节基因的表达谱分析.北京:中国林业科学研究院,2006
[9]	Chang S, Puryear J, Cairney J. A simple and eficient method for isolating RNA from pine trees[J]. Plant Molecular Biol Reporter, 1993,11(2):113
[10]	周琳, 王雁, 彭镇华.牡丹查耳酮合酶基因Ps-CHS1的克隆及其组织特异性表达[J].园艺学报, 2010,37(8): 1295-1302
[11]	Eulgem T, Rushton P J, Robatzek S, et al. The WRKY superfamily of plant transcritional factors[J].Trends Plant Sci, 2000,5(5): 199-206
[12]	范正琪,李纪元,田敏,等.麻疯树磷酸烯酮式丙酮酸羧化酶pepc基因全长cDNA克隆及序列分析[J].林业科学研究,2010,23(3):349-354
[13]	付乾堂, 余迪求. 拟南芥AtWRKY25、AtWRKY26和AtWRKY33在非生物胁迫条件下的表达分析[J].遗传,2010, 32 (8):848-856
[14]	Asai T, Tena G, Plotnikova J, et al. MAP kinase signalling cascadein Arabidopsis innateimmunity[J]. Nature, 2002,415(6875):977-983
[15]	Kim C Y, Zhang S. Activation of a mitogen-activated protein kinase cascade induces WRKY family of transcription factors and defense genes in tobacco[J]. PLant J, 2004, 38(1): 142-151
[16]	田云, 卢向阳, 彭丽莎, 等. 植物WRKY转录因子结构特点及其生物学功能[J].遗传,2006, 28 (12):1607-1612

Cloning and Expression Analysis of ChWRKY2 from Corylus heterophylla under Low Temperature Stress

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