Tissue Culture Technique of Acacia mangium Elite Trees

HUANG Lie-jian; CHEN Zu-xu; ZHANG Sai-qun; LIANG Ri-gao

Taking Acacia mangium elite trees as explants, the tissue culture technique system for dormant bud of 3-5 year-old elite trees was established. The system includes the germination-inducing of the dormant buds, the multiplying of shoots, the rooting of adventitious shoots, and the pre-treating and transplanting of seedlings. The medium MS + Sucrose 30 g·L-1 + BA 0.5 mg·L-1 + NAA 0.1 mg·L-1 was used for inducing the dormant buds; the medium MS + BA 1.0 mg·L-1 + NAA 0.05 mg·L-1 was used for multiplying; while the medium 1/2 MS + IBA 2.0 mg·L-1 + NAA 0.5 mg·L-1 was used for rooting. The results revealed that it was viable to producing field seedlings with micropropagation. Although the branches with dormant buds harbored many kinds of microbes and the adventitious shoots were not easy to root, 20% to 30% healthy germination could be yielded and the rooting rate of adventitious shoots could be higher than 85%. Pretreated with ABT powder (rooting hormone), both the rooting rate and survival rate of adventitious shoots were nearly 100%.

1. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, Guangdong, China

2. School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China

3. Research Institute of Forestry of Jiangmen City, Jiangmen 529000, Guangdong, China

Received Date: 2010-08-21

Available Online: 2012-04-13

Abstract: Taking Acacia mangium elite trees as explants, the tissue culture technique system for dormant bud of 3-5 year-old elite trees was established. The system includes the germination-inducing of the dormant buds, the multiplying of shoots, the rooting of adventitious shoots, and the pre-treating and transplanting of seedlings. The medium MS + Sucrose 30 g·L-1 + BA 0.5 mg·L-1 + NAA 0.1 mg·L-1 was used for inducing the dormant buds; the medium MS + BA 1.0 mg·L-1 + NAA 0.05 mg·L-1 was used for multiplying; while the medium 1/2 MS + IBA 2.0 mg·L-1 + NAA 0.5 mg·L-1 was used for rooting. The results revealed that it was viable to producing field seedlings with micropropagation. Although the branches with dormant buds harbored many kinds of microbes and the adventitious shoots were not easy to root, 20% to 30% healthy germination could be yielded and the rooting rate of adventitious shoots could be higher than 85%. Pretreated with ABT powder (rooting hormone), both the rooting rate and survival rate of adventitious shoots were nearly 100%.

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Reference (8)

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Tissue Culture Technique of Acacia mangium Elite Trees

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References

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