Study on Storage and Bourgeoning of Spruce Pollen

MA Jian-wei; WANG Jun-hui; ZHANG Shou-gong; ZHAO Qiu-ling; HAN Yun-hua; ZHANG Zeng-shun

The effects of sucrose concentration, pH value and storage conditions on germination and pollen tube growth of in vitro cultured spruce were studied, and the ingredients of culture medium were optimized. The results showed that the effect of sucrose concentration and pH value on spruce pollen’s germination was significant, and also a clear threshold effect existed. Low or high concentration of sucrose was not conducive to the pollen’s germination, it was found that 10% of which was more suitable to the in vitro culture of spruce pollen; different spruce species required different concentrations of sucrose medium, 7.5% of sucrose concentration medium was better to pollen tube germination of Picea asperata, but 10% of which was the best medium to that of Picea abies. Both pH 4.5 and 6.5 had restrained action on pollen germinating rate of spruce, and the growth of pollen tubes. Under dark culture conditions, the P. abies began to germinate in the 6 h, but the P. asperata germinated in 8 h, the P. abies was 2 hours in advance and in the mass germinating power was higher than that of P. asperata; the growth process of P. abies was 20-22 h, and that of P. asperata was about 16 h; The pollen tubes of P. abies was over 10 μm longer in length than that of P. asperata. There were significant differences of germinating power and length of pollen tubes between genotypes of two different individuals of both spruces. The storage methods had great influence on the viability of pollen. In the process of pollen preservation, temperature was the key factor, cryopreservation was better than cold storage and room temperature preservation was unfavorable.

1. Xiaolongshan Research Institute of Forest Science and Technology, Tianshui 741022, Gansu, China

2. Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, Ch

Received Date: 2010-12-16

Available Online: 2012-06-13

Abstract: The effects of sucrose concentration, pH value and storage conditions on germination and pollen tube growth of in vitro cultured spruce were studied, and the ingredients of culture medium were optimized. The results showed that the effect of sucrose concentration and pH value on spruce pollen’s germination was significant, and also a clear threshold effect existed. Low or high concentration of sucrose was not conducive to the pollen’s germination, it was found that 10% of which was more suitable to the in vitro culture of spruce pollen; different spruce species required different concentrations of sucrose medium, 7.5% of sucrose concentration medium was better to pollen tube germination of Picea asperata, but 10% of which was the best medium to that of Picea abies. Both pH 4.5 and 6.5 had restrained action on pollen germinating rate of spruce, and the growth of pollen tubes. Under dark culture conditions, the P. abies began to germinate in the 6 h, but the P. asperata germinated in 8 h, the P. abies was 2 hours in advance and in the mass germinating power was higher than that of P. asperata; the growth process of P. abies was 20-22 h, and that of P. asperata was about 16 h; The pollen tubes of P. abies was over 10 μm longer in length than that of P. asperata. There were significant differences of germinating power and length of pollen tubes between genotypes of two different individuals of both spruces. The storage methods had great influence on the viability of pollen. In the process of pollen preservation, temperature was the key factor, cryopreservation was better than cold storage and room temperature preservation was unfavorable.

HTML

Reference (21)

[1]	孙颖,孙大业.花粉萌发和花粉管生长发育的信号转导[J].植物学报,2001,43(12):211-221
[2]	郝怀庆,王钦丽,陈艳梅,等.花粉管细胞壁结构及胞质运动[J].植物学通报, 2003,20(3):270-279
[3]	张绍铃,陈迪新,康琅,等.培养基组分及pH 值对梨花粉萌发和花粉管生长的影响[J].西北植物学报,2005,25(2):225-230
[4]	龚明,杨中汉,曹宗巽.钙对花粉萌发的启动效应和对花粉管生长的调节作用[J].北京大学学报:自然科学版,1995,31(2):238-249
[5]	姚成义,赵洁.钙和硼对蓝猪耳花粉萌发及花粉管生长的影响[J].武汉植物学研究,2004,22(1):1-7
[6]	龚明,曹宗巽.钙和钙调素对花粉萌发和花粉管生长的调节[J].植物生理学通讯,1995,31(5):321-328
[7]	田翠婷,吕洪飞,王锋,等.培养基组分对青扦离体花粉萌发和花粉管生长的影响[J].北京林业大学学报,2007,29(1)1:47-52
[8]	尹佳蕾,赵惠恩.花粉生活力影响因素及花粉贮藏概述[J].中国农学通报,2005,21(4):110-113
[9]	金鑫,许明子,李美善,等.不同蔗糖浓度对高山红景天花粉发芽率及花粉管生长的影响[J].延边大学农学学报,2007,29(1):37-39
[10]	陈品良,金炜.木本植物花粉生活力测定及生活力下降原因探讨[J].植物学通报,1990,7(1): 64-66
[11]	赵长星,刘成连.培养基种类及蔗糖浓度对部分果树花粉发芽率的影响[J].河北林果研究,2001,16(3):240-243
[12]	王素玲,姜殿勤,王小红.蔗糖质量分数和pH值对樟子松花粉发芽率的影响[J].吉林林业科技,2005,34(2):7-9
[13]	刘勇,刘善军,邱东萍.甜柿花粉发芽率的影响因子与发芽动态研究[J].江西科学,2001,19(4):218-221
[14]	尹恒,唐前瑞,王慧颖,等.麝香百合花粉发芽率适宜培养基及其贮藏研究[J] .长江大学学报(自然版)农学卷,2007,4(3):34-36
[15]	蒋祥娥,汪建亚,熊冬莲,等.适合马尾松花粉发芽的培养基试验[J].湖北林业科技,2001(2):6-10
[16]	吕世友.云杉和油松花粉发育及其原叶细胞程序化死亡的研究 .长春:中国人民解放军军需大学,2001:17-21
[17]	葛莉莉,赵文义,阿腾戈,等.云杉花粉生活力及贮藏方法试验研究[J].内蒙古林业科技,2007,33(4):33-36
[18]	Pierson E S,Li Y Q,ZHang H Q,et al.Pulsatory growth of pollen tube:Investigation of a possible relationship with the periodic distribution of cell wall components[J].Acta Botany Neerl,1995,44(2):121-128
[19]	Lanteri S, Belletti P, Lotito S.Storage of pollen of norway spruce and different pine species[J].Silvae Genetica,1993,42(2-3):104-109
[20]	Margaret A.Wheeler,Jen A McComb.In vitro pollen viability and pollen storage in Eucaylptus marginata(Myrtaceae)[J].Australian Forestry,2006,69(1):32-37
[21]	Holdaway-Clarket T,Hepler P.Control of pollen tube growth:role of ion gradients and fluxes[J].New Phytologist,2003(3):539-563

Study on Storage and Bourgeoning of Spruce Pollen

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views