Population Regeneration and Genetic Diversity of Spruce (Picea koraiensis) in Heilongjiang Eastern Mountain Area

ZHAO Li-ling; SUN Long; WANG Qing-gui

The survey plots were selected for natural regeneration of Picea koraiensis in the Northeastern part of 12 Picea koraiensis provenances distribution area. In order to reveal the genetic diversity of P.koraiensis, 144 samples from 12 provenances were examined by using RAPD markers. Their genetic diversity characteristics were analyzed based on the population regeneration results in this study. The results could provide the evidences in population level and molecular level for the study on the declining mechanism of spruce. The result showed that there were higher genetic variations among the provenances of P.koaiensis at DNA level. The proportion of total polymorphic loci reached 98.81%. Also, Shannon’s Information Index and Nei’s Gene Diversity Index were used to estimate the genetic diversities for the provenances of P. koraiensis, the Nei (H) was 0.363 2, the Shannon index (I) was 0.540 5. The genetic differentiations for inter-provenance and intra-provenance were 27.72% and 72.28%, the genetic differentiation index (Gst) among these provenances was 0.277 2, the gene flow coefficient was 1.304 0. It also showed that there was a close relationship among different provenances, varying from 0.804 6 to 0.923 7. On the basis of cluster analysis of genetic distance, 12 provenances could be divided into three groups, the first group includes the provenances of Xinqing, Wuyiling and Hongxing; the second includes the provenances of Wuying, Youhao, Meixi, Wumahe, Dailing, Nancha and Shuangfeng; and the last group contains the provenances of Mengkeshan and Talin. Population regeneration data showed that: Daxing’anling, Xinqing, Wuyiling and Hongxing provenances in Xiaoxing’anling are progressing populations, Wuying, Youhao, Meixi, Wumahe, Dailing, Nancha and Shuangfeng provenances in Xiaoxing’anling are declining population, These result can provide a basis to re-allocate the districts for the optimal distribution of P.koraiensis. The results may further provide a data base for the declining mechanism of spruce, and also provide a scientific basis for spruce genetic diversity conservation and ecological restoration.

1. Institute of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, China

2. Institute of Agricultural Resources and Environment, Heilongjiang University, Harbin 150080, Heilongjiang, China

Received Date: 2011-08-20

Available Online: 2012-06-13

Abstract: The survey plots were selected for natural regeneration of Picea koraiensis in the Northeastern part of 12 Picea koraiensis provenances distribution area. In order to reveal the genetic diversity of P.koraiensis, 144 samples from 12 provenances were examined by using RAPD markers. Their genetic diversity characteristics were analyzed based on the population regeneration results in this study. The results could provide the evidences in population level and molecular level for the study on the declining mechanism of spruce. The result showed that there were higher genetic variations among the provenances of P.koaiensis at DNA level. The proportion of total polymorphic loci reached 98.81%. Also, Shannon’s Information Index and Nei’s Gene Diversity Index were used to estimate the genetic diversities for the provenances of P. koraiensis, the Nei (H) was 0.363 2, the Shannon index (I) was 0.540 5. The genetic differentiations for inter-provenance and intra-provenance were 27.72% and 72.28%, the genetic differentiation index (Gst) among these provenances was 0.277 2, the gene flow coefficient was 1.304 0. It also showed that there was a close relationship among different provenances, varying from 0.804 6 to 0.923 7. On the basis of cluster analysis of genetic distance, 12 provenances could be divided into three groups, the first group includes the provenances of Xinqing, Wuyiling and Hongxing; the second includes the provenances of Wuying, Youhao, Meixi, Wumahe, Dailing, Nancha and Shuangfeng; and the last group contains the provenances of Mengkeshan and Talin. Population regeneration data showed that: Daxing’anling, Xinqing, Wuyiling and Hongxing provenances in Xiaoxing’anling are progressing populations, Wuying, Youhao, Meixi, Wumahe, Dailing, Nancha and Shuangfeng provenances in Xiaoxing’anling are declining population, These result can provide a basis to re-allocate the districts for the optimal distribution of P.koraiensis. The results may further provide a data base for the declining mechanism of spruce, and also provide a scientific basis for spruce genetic diversity conservation and ecological restoration.

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Reference (19)

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Population Regeneration and Genetic Diversity of Spruce (Picea koraiensis) in Heilongjiang Eastern Mountain Area

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