Changes of Endogenous Hormones and Anatomical Structure of Rhododendron stamineum During Cutting Propagation

LI Chao-chan; ZHAO Yun-long; ZHANG Dong-lin; WU Hua-mei; CHEN Xun

The semi-hardwood stems of wild Rhododendron stamineum were collected and treated with KIBA, KNAA and Hormodin. The contents of endogenous hormones IAA, GA3, ZR, and ABA were measured and the stems were scanned with SEM during the rooting period. The results indicated that double dips (quick dipped into liquid KNAA (3 000 mg·L-1) and then the powder Hormodin #1 (1 000 mg·L-1)) had a significant effect on rooting and resulted in the highest rooting rate of 81.25%. During the rooting process, higher concentration of endogenous IAA contents was conductive to the formation of root primordium differentiation, GA3 content showed a positive correlation with the cuttings callus induction and adventitious root formation, and low levels of ABA should promote rooting of cuttings. Cuttings needed endogenous ZR during root primordium differentiation and callus formation and it could synthesize own ZR after root initiated. It is necessary to supplement carbohydrate during rotting period through the research of starch grains, the emergence of cluster-like crystals indicated that the mechanism of Ca regulation has been established.

1. College of Forestry, Guizhou University, Guiyang 550025, Guizhou, China

2. Guizhou Academy of Sciences, Guiyang 550001, Guizhou, China

3. Department of Plant, Soil and Environmental Sciences, The University of Maine, Orono, Maine 04469, USA

Received Date: 2011-12-07

Available Online: 2012-06-13

Abstract: The semi-hardwood stems of wild Rhododendron stamineum were collected and treated with KIBA, KNAA and Hormodin. The contents of endogenous hormones IAA, GA3, ZR, and ABA were measured and the stems were scanned with SEM during the rooting period. The results indicated that double dips (quick dipped into liquid KNAA (3 000 mg·L-1) and then the powder Hormodin #1 (1 000 mg·L-1)) had a significant effect on rooting and resulted in the highest rooting rate of 81.25%. During the rooting process, higher concentration of endogenous IAA contents was conductive to the formation of root primordium differentiation, GA3 content showed a positive correlation with the cuttings callus induction and adventitious root formation, and low levels of ABA should promote rooting of cuttings. Cuttings needed endogenous ZR during root primordium differentiation and callus formation and it could synthesize own ZR after root initiated. It is necessary to supplement carbohydrate during rotting period through the research of starch grains, the emergence of cluster-like crystals indicated that the mechanism of Ca regulation has been established.

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Changes of Endogenous Hormones and Anatomical Structure of Rhododendron stamineum During Cutting Propagation

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