Isolation and Comparison of Inhibitory Effect of Antimicrobial Peptides from Blaps rhynchoptera Larvae under Induced and Non-induced Conditions

SUN Long; FENG Ying; HE Zhao; CHEN Zhi-Yong; ZHAO Min; HE Rui

Volume 25 Issue 3

Article Contents

Turn off MathJax

Article Navigation > Forest Research > 2012 > 25(3): 373-377

Citation:

Isolation and Comparison of Inhibitory Effect of Antimicrobial Peptides from Blaps rhynchoptera Larvae under Induced and Non-induced Conditions

1.
Research Institute of Resources Insects, Chinese Academy of Forestry, Key Laboratory of Cultivation and Utilization of Resource Insects, State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2012-01-12

Abstract

Antimicrobial peptide which plays a key role in the innate immune system of insect, is a kind of cationic alkaline peptide with low molecule weight. It is so effective against bacteria that it can be expected to develop as a new antibiotic. In this study, the authors compared the inhibitory effect of antimicrobial peptides of Blaps rhynchoptera Fairmaire larvae at induced treatment with non-induced treatment during isolation and purification procedures. The result indicated that both crude extracts of the two groups had no inhibitory effect on indicator bacteria at first. But after gel chromatography, the fraction DZP1 from non-induced group showed anti-bacteria activities against Staphylococcus aureus and Bacillus subtilis. Meanwhile the fraction YDP1 from induced group displayed anti-bacteria activities against Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa. Moreover, YDP1 had stronger activity than DZP1 and its anti-bacteria spectrum was broadened. Based on the analysis of Tricine-SDS-PAGE, the components of DZP1 and YDP1 were similar, both composed of small peptides no less than 20 kD. The results suggested that the antimicrobial peptide could be obtained from both induced and non-induced insects and the inhibitory effect against microbes would be stronger after challenging with bacteria.
- Blaps rhynchoptera,
- antimicrobial peptide,
- induced,
- inhibitory effect

References

[1]	Zasloff M. Antimicrobial peptides of multicellular organisms[J]. Nature, 2002,415(6870):389-395
[2]	Bulet P, Hetru C, Dimarcq J L, et al. Antimicrobial peptides in insects; structure and function[J]. Dev Comp Immunol, 1999,23(4-5):329-344
[3]	Hancock R E, Lehrer R. Cationic peptides: a new source of antibiotics[J]. Trends Biotechnol, 1998,16(2):82-88
[4]	吴甜甜, 杨洁. 天然抗菌肽的研究进展及应用前景[J]. 生物技术通报, 2009(1):27-30
[5]	Bulet P, Stocklin R. Insect antimicrobial peptides: structures, properties and gene regulation[J]. Protein Pept Lett, 2005,12(1):3-11
[6]	Huang L, Cheng T, Xu P, et al. A genome-wide survey for host response of silkworm, Bombyx mori during pathogen Bacillus bombyseptieus infection[J]. Plos One, 2009,4(12):e8098
[7]	Haine E R, Moret Y, Siva-Jothy M T, et al. Antimicrobial defense and persistent infection in insects[J]. Science, 2008,322(5905):1257-1259
[8]	Nguyen L T, Haney E F, Vogel H J. The expanding scope of antimicrobial peptide structures and their modes of action[J]. Trends in Biotechnology, 2011,29(9):464-472
[9]	张兰胜, 夏从龙, 杨永寿, 等. 彝药喙尾琵琶甲的研究进展[J]. 时珍国医国药, 2009,20(12):3113-3114
[10]	赵敏, 陈晓鸣, 孙龙, 等. 云南省喙尾琵琶甲分布及生态环境调查[J]. 林业科学研究, 2007,20(3):356-362
[11]	施贵荣, 肖培云, 洪小凤, 等. 喙尾琵琶甲提取物体外抗菌作用初步研究[J]. 时珍国医国药, 2011,22(3):622-623
[12]	施贵荣, 庄孝龙, 孙会仙, 等. 喙尾琵琶甲抗菌活性的研究[J]. 大理学院学报:综合版, 2010,9(12):18-19
[13]	李蕾, 罗氚芸, 等. 云南琵琶甲抗菌活性成分研究[J]. 中草药, 2001,32(3):197-199
[14]	刘勇, 罗氚芸, 李蕾, 等. 云南琵琶甲防御性分泌物抗菌活性及GC-MS分析[J]. 云南大学学报:自然科学版, 2000,22(3):217-219
[15]	罗氚芸, 刘勇. 云南琵琶甲脂溶性抗菌活性成分的研究[J]. 中草药, 1999,30(8):566-567
[16]	罗建蓉, 何江波, 张桢, 等. 药用昆虫喙尾琵琶甲化学成分研究[J]. 中成药, 2010(11):2013-2014
[17]	罗氚芸, 刘勇. 云南民间药用琵琶甲无机元素及氨基酸分析[J]. 氨基酸和生物资源, 1999,21(3):38-40
[18]	郭宝华, 何钊, 冯颖, 等. 三种昆虫甲壳素提取与分析[J]. 林业科学研究, 2008,21(4):561-565
[19]	张建社, 褚武英, 陈韬. 蛋白质分离与纯化技术[M]. 北京: 军事医学科学出版社, 2009
[20]	Schägger H. Tricine-SDS-PAGE[J]. Nature Protocols, 2006,1(1):16-22
[21]	叶英妩, 王毓三, 申子瑜. 全国临床检验操作规程[M]. 第3版. 南京: 东南大学出版社, 2006
[22]	Yang W, Cheng T, Ye M, et al. Functional divergence among silkworm antimicrobial peptide paralogs by the activities of recombinant proteins and the induced expression profiles[J]. Plos One, 2011,6(3):e18109
[23]	Hetru C, Troxler L, Hoffmann J A. Drosophila melanogaster antimicrobial defense[J]. J Infect Dis, 2003,187(Suppl 2):S327-S334
[24]	Dang X L, Wang Y S, Huang Y D, et al. Purification and characterization of an antimicrobial peptide, insect defensin, from immunized house fly (Diptera: Muscidae)[J]. J Med Entomol, 2010,47(6):1141-1145
[25]	Cho W L, Fu Y C, Chen C C, et al. Cloning and characterization of cDNAs encoding the antibacterial peptide, defensin A, from the mosquito, Aedes aegypti[J]. Insect Biochemistry and Molecular Biology, 1996,26(4):395-402
[26]	Hancock R E, Sahl H G. Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies[J]. Nat Biotechnol, 2006,24(12):1551-1557
[27]	Fields G B. Peptide characterization and application protocols[M]. Totowa, N J.: Humana, 2007
[28]	许兵红, 董卫华, 陈萍, 等. 家蝇抗菌物质诱导及高温处理后的抑菌活性[J]. 中国媒介生物学及控制杂志, 2009,20(3):201-205
[29]	陶淑霞, 韩静菲, 李玉. 诱导源及其诱导剂量对黄粉虫幼虫血淋巴抗菌活性的影响[J]. 吉林农业大学学报, 2010,32(2):145-148

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(3065) PDF downloads(68) Cited by()

Proportional views

Isolation and Comparison of Inhibitory Effect of Antimicrobial Peptides from Blaps rhynchoptera Larvae under Induced and Non-induced Conditions

1. Research Institute of Resources Insects, Chinese Academy of Forestry, Key Laboratory of Cultivation and Utilization of Resource Insects, State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2012-01-12

Available Online: 2012-06-13

Abstract: Antimicrobial peptide which plays a key role in the innate immune system of insect, is a kind of cationic alkaline peptide with low molecule weight. It is so effective against bacteria that it can be expected to develop as a new antibiotic. In this study, the authors compared the inhibitory effect of antimicrobial peptides of Blaps rhynchoptera Fairmaire larvae at induced treatment with non-induced treatment during isolation and purification procedures. The result indicated that both crude extracts of the two groups had no inhibitory effect on indicator bacteria at first. But after gel chromatography, the fraction DZP1 from non-induced group showed anti-bacteria activities against Staphylococcus aureus and Bacillus subtilis. Meanwhile the fraction YDP1 from induced group displayed anti-bacteria activities against Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa. Moreover, YDP1 had stronger activity than DZP1 and its anti-bacteria spectrum was broadened. Based on the analysis of Tricine-SDS-PAGE, the components of DZP1 and YDP1 were similar, both composed of small peptides no less than 20 kD. The results suggested that the antimicrobial peptide could be obtained from both induced and non-induced insects and the inhibitory effect against microbes would be stronger after challenging with bacteria.

HTML

Reference (29)

[1]	Zasloff M. Antimicrobial peptides of multicellular organisms[J]. Nature, 2002,415(6870):389-395
[2]	Bulet P, Hetru C, Dimarcq J L, et al. Antimicrobial peptides in insects; structure and function[J]. Dev Comp Immunol, 1999,23(4-5):329-344
[3]	Hancock R E, Lehrer R. Cationic peptides: a new source of antibiotics[J]. Trends Biotechnol, 1998,16(2):82-88
[4]	吴甜甜, 杨洁. 天然抗菌肽的研究进展及应用前景[J]. 生物技术通报, 2009(1):27-30
[5]	Bulet P, Stocklin R. Insect antimicrobial peptides: structures, properties and gene regulation[J]. Protein Pept Lett, 2005,12(1):3-11
[6]	Huang L, Cheng T, Xu P, et al. A genome-wide survey for host response of silkworm, Bombyx mori during pathogen Bacillus bombyseptieus infection[J]. Plos One, 2009,4(12):e8098
[7]	Haine E R, Moret Y, Siva-Jothy M T, et al. Antimicrobial defense and persistent infection in insects[J]. Science, 2008,322(5905):1257-1259
[8]	Nguyen L T, Haney E F, Vogel H J. The expanding scope of antimicrobial peptide structures and their modes of action[J]. Trends in Biotechnology, 2011,29(9):464-472
[9]	张兰胜, 夏从龙, 杨永寿, 等. 彝药喙尾琵琶甲的研究进展[J]. 时珍国医国药, 2009,20(12):3113-3114
[10]	赵敏, 陈晓鸣, 孙龙, 等. 云南省喙尾琵琶甲分布及生态环境调查[J]. 林业科学研究, 2007,20(3):356-362
[11]	施贵荣, 肖培云, 洪小凤, 等. 喙尾琵琶甲提取物体外抗菌作用初步研究[J]. 时珍国医国药, 2011,22(3):622-623
[12]	施贵荣, 庄孝龙, 孙会仙, 等. 喙尾琵琶甲抗菌活性的研究[J]. 大理学院学报:综合版, 2010,9(12):18-19
[13]	李蕾, 罗氚芸, 等. 云南琵琶甲抗菌活性成分研究[J]. 中草药, 2001,32(3):197-199
[14]	刘勇, 罗氚芸, 李蕾, 等. 云南琵琶甲防御性分泌物抗菌活性及GC-MS分析[J]. 云南大学学报:自然科学版, 2000,22(3):217-219
[15]	罗氚芸, 刘勇. 云南琵琶甲脂溶性抗菌活性成分的研究[J]. 中草药, 1999,30(8):566-567
[16]	罗建蓉, 何江波, 张桢, 等. 药用昆虫喙尾琵琶甲化学成分研究[J]. 中成药, 2010(11):2013-2014
[17]	罗氚芸, 刘勇. 云南民间药用琵琶甲无机元素及氨基酸分析[J]. 氨基酸和生物资源, 1999,21(3):38-40
[18]	郭宝华, 何钊, 冯颖, 等. 三种昆虫甲壳素提取与分析[J]. 林业科学研究, 2008,21(4):561-565
[19]	张建社, 褚武英, 陈韬. 蛋白质分离与纯化技术[M]. 北京: 军事医学科学出版社, 2009
[20]	Schägger H. Tricine-SDS-PAGE[J]. Nature Protocols, 2006,1(1):16-22
[21]	叶英妩, 王毓三, 申子瑜. 全国临床检验操作规程[M]. 第3版. 南京: 东南大学出版社, 2006
[22]	Yang W, Cheng T, Ye M, et al. Functional divergence among silkworm antimicrobial peptide paralogs by the activities of recombinant proteins and the induced expression profiles[J]. Plos One, 2011,6(3):e18109
[23]	Hetru C, Troxler L, Hoffmann J A. Drosophila melanogaster antimicrobial defense[J]. J Infect Dis, 2003,187(Suppl 2):S327-S334
[24]	Dang X L, Wang Y S, Huang Y D, et al. Purification and characterization of an antimicrobial peptide, insect defensin, from immunized house fly (Diptera: Muscidae)[J]. J Med Entomol, 2010,47(6):1141-1145
[25]	Cho W L, Fu Y C, Chen C C, et al. Cloning and characterization of cDNAs encoding the antibacterial peptide, defensin A, from the mosquito, Aedes aegypti[J]. Insect Biochemistry and Molecular Biology, 1996,26(4):395-402
[26]	Hancock R E, Sahl H G. Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies[J]. Nat Biotechnol, 2006,24(12):1551-1557
[27]	Fields G B. Peptide characterization and application protocols[M]. Totowa, N J.: Humana, 2007
[28]	许兵红, 董卫华, 陈萍, 等. 家蝇抗菌物质诱导及高温处理后的抑菌活性[J]. 中国媒介生物学及控制杂志, 2009,20(3):201-205
[29]	陶淑霞, 韩静菲, 李玉. 诱导源及其诱导剂量对黄粉虫幼虫血淋巴抗菌活性的影响[J]. 吉林农业大学学报, 2010,32(2):145-148

Isolation and Comparison of Inhibitory Effect of Antimicrobial Peptides from Blaps rhynchoptera Larvae under Induced and Non-induced Conditions

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

Isolation and Comparison of Inhibitory Effect of Antimicrobial Peptides from Blaps rhynchoptera Larvae under Induced and Non-induced Conditions

HTML

Catalog

Export File

Citation

Format

Content