Induction and Differentiation of Four Eucalyptus Clones in vitro Culture

QIU Zhen-fei; ZENG Bing-shan; LI Xiang-yang; LIU Ying; ZHA Zhi-gang

The in vitro plantlet leaves and stems of 4 elite Eucalyptus clones commonly cultivated in production were used as explants to study the callus induction and differentiation under different callus induction time, in order to establish an effective callus regeneration system. The results showed: The clones Eg5 and DH201-2 leaf callus inducted for 45 d were suitable for differentiation of adventitious buds, with the highest regeneration rate of 70.0% and 63.3% respectively. 20 d and 15 d were the best induction times for leaf callus inducting and stem callus inducting of clone GL9, with the regeneration rates of 66.7% and 63.3% respectively. The best regeneration rate of DH3229 was 16.7% from stem callus inducted for 30 d. There were significant differences in regeneration rate among the 4 clones. Clones Eg5, DH201-2, and GL9 were easy to regenerate compared with DH3229. No obvious difference was found in regeneration rate of leaf callus and stem callus from same clone.

1. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, Guangdong, China

2. Huangshan Forest Pest Control Quarantine Station, Huangshan 245000, Anhui, China

Received Date: 2011-06-16

Available Online: 2012-08-20

Abstract: The in vitro plantlet leaves and stems of 4 elite Eucalyptus clones commonly cultivated in production were used as explants to study the callus induction and differentiation under different callus induction time, in order to establish an effective callus regeneration system. The results showed: The clones Eg5 and DH201-2 leaf callus inducted for 45 d were suitable for differentiation of adventitious buds, with the highest regeneration rate of 70.0% and 63.3% respectively. 20 d and 15 d were the best induction times for leaf callus inducting and stem callus inducting of clone GL9, with the regeneration rates of 66.7% and 63.3% respectively. The best regeneration rate of DH3229 was 16.7% from stem callus inducted for 30 d. There were significant differences in regeneration rate among the 4 clones. Clones Eg5, DH201-2, and GL9 were easy to regenerate compared with DH3229. No obvious difference was found in regeneration rate of leaf callus and stem callus from same clone.

HTML

Reference (9)

[1]	欧阳权,曾炼武,李洁汉. 桉树组培育苗新技术[J]. 广西科学,1994,1(3):49-59
[2]	石大兴,石轶松,王米力,等. 巨桉下胚轴诱导不定芽与植株再生的研究[J]. 四川农业大学学报, 2002,20(3):184-187
[3]	谭得冠,庄南生,黄华孙. 刚果12号桉愈伤组织的诱导与再生植株快繁体系的构建[J]. 热带作物学报,2005,26(3):24-28
[4]	宛淑艳,陈文渊,何晓玲. 尾叶桉茎段再生系统建立[J].基因组学与应用生物学,2010,29(5):937-942
[5]	Luis P B C, Adriane C M G, Silvia B R C. Plant regeneration from seedling explants of Eucalyptus grandis × E. urophylla [J]. Plant Cell, Tissue and Organ Culture, 1999, 56: 17-23
[6]	Subbaiah M M,Minocha S C. Shoot regeneration from stem and leaf callus of Eucalyptus tereticornis [J]. Plant Cell Reports, 1999,9:370-373
[7]	范春节,曾炳山,裘珍飞,等. 尾赤桉叶片及茎段的离体培养与植株再生[J]. 福建林学院学报,2009,29(1):74-78
[8]	范春节,曾炳山,裘珍飞,等. 尾赤桉DH201-2遗传转化体系建立的研究[J]. 浙江林业科技,2009,29(4):15-20
[9]	裘珍飞,曾炳山,李湘阳,等. TDZ对巨尾桉(GL9)胚性愈伤组织诱导和再生的影响[J]. 林业科学研究,2009,22(5):740-743

Induction and Differentiation of Four Eucalyptus Clones in vitro Culture

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views