Molecular Identification of Bamboo Planthoppers (Hemiptera:Delphacidae) Based on Mitochondrial 16S rDNA Gene Sequences

HOU Xiao-hui; CHEN Xiang-sheng

Approximately 480 base pairs of mitochondrial 16S rDNA gene were sequenced for 15 species of Delphacidae. Of 480 bp mitochondrial 16S rDNA gene sequences, the percentage of A+T was about 77.5%, and 190 nucleotide sites were substituted (about 39.6%). The genetic distance of outgroup and ingroup, intergenus, interspecies and intraspecies were 0.317, 0.209, 0.132 and 0.013 respectively. The molecular phylogenetic trees constructed by parsimony method and neighbor-joining method suggest that the ingroup and outgroup are independent evolutionary branches. The genus and species formed monophyletic groups respectively, so accuracy identification is possible through the research of sequences. These results are identical with that of morphology.

1. Institute of Insects, Guizhou University/Guizhou Key Laboratory for Plant Pest Management of Mountainous Region, Guiyang 550025, Guizhou, China

2. Zunyi Medical College, Zunyi 563003, Guizhou, China

Received Date: 2012-04-17

Abstract: Approximately 480 base pairs of mitochondrial 16S rDNA gene were sequenced for 15 species of Delphacidae. Of 480 bp mitochondrial 16S rDNA gene sequences, the percentage of A+T was about 77.5%, and 190 nucleotide sites were substituted (about 39.6%). The genetic distance of outgroup and ingroup, intergenus, interspecies and intraspecies were 0.317, 0.209, 0.132 and 0.013 respectively. The molecular phylogenetic trees constructed by parsimony method and neighbor-joining method suggest that the ingroup and outgroup are independent evolutionary branches. The genus and species formed monophyletic groups respectively, so accuracy identification is possible through the research of sequences. These results are identical with that of morphology.

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Reference (16)

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Molecular Identification of Bamboo Planthoppers (Hemiptera:Delphacidae) Based on Mitochondrial 16S rDNA Gene Sequences

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通讯作者: 陈斌, bchen63@163.com

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