Isolation of Ethyl Acetate Extract from Periplaneta americana and Its Antimicrobial Activity

WANG Kui; FENG Ying; SUN Long; HE Zhao; CHEN Zhi-yong

Antimicrobial chemical components, which isolated from the ethyl acetate extract of Periplaneta americana L., were tested for the ability to inhibit Staphyloccocus aureus, Escherichia coli, etc., based on bioassay-guided method. The results indicated that the ethyl acetate extract of P. americana showed some inhibiting effects to all gram-positive bacteria tested, and the best inhibition effect was against Bacillus subtilis. Nine factions were obtained after silica gel column chromatography. Among them, the faction Fr7 showed inhibiting effects to all bacteria tested. Applying the isolation method of silica gel column and gel chromatography, seven unsaturated fatty acids compounds were isolated from the faction Fr7 and Fr8. All these compounds showed the best inhibiting effects against Micrococcus luteus. The results suggested that unsaturated fatty acids were antimicrobial chemical components of the ethyl acetate extract from P.americana. The inhibition effects of the unsaturated fatty acids against gram-positive bacteria were stronger than against gram-negative bacteria.

1. Research Institute of Resources Insects, Chinese Academy of Forestry, Key Laboratory of Cultivation and Utilization of Resources Insects, State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2012-11-23

Abstract: Antimicrobial chemical components, which isolated from the ethyl acetate extract of Periplaneta americana L., were tested for the ability to inhibit Staphyloccocus aureus, Escherichia coli, etc., based on bioassay-guided method. The results indicated that the ethyl acetate extract of P. americana showed some inhibiting effects to all gram-positive bacteria tested, and the best inhibition effect was against Bacillus subtilis. Nine factions were obtained after silica gel column chromatography. Among them, the faction Fr7 showed inhibiting effects to all bacteria tested. Applying the isolation method of silica gel column and gel chromatography, seven unsaturated fatty acids compounds were isolated from the faction Fr7 and Fr8. All these compounds showed the best inhibiting effects against Micrococcus luteus. The results suggested that unsaturated fatty acids were antimicrobial chemical components of the ethyl acetate extract from P.americana. The inhibition effects of the unsaturated fatty acids against gram-positive bacteria were stronger than against gram-negative bacteria.

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Reference (19)

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Isolation of Ethyl Acetate Extract from Periplaneta americana and Its Antimicrobial Activity

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