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Cloning and Express Analysis of PeAP2 Gene and Its Promoter in Phyllostachys edulis

  • Received Date: 2012-12-03
  • APETALA2 (AP2) gene plays an important role in plant development. A full-length cDNA sequence of AP2 homologous gene was cloned from Phyllostachys edulis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods, and named as PeAP2. The PeAP2 is 1 750 bp and consists of a 5'-untranslated region (UTR) of 106 bp, a 3'-UTR of 174 bp and an opening reading frame of 1 470 bp. The sequence analysis showed that PeAP2 encoded a protein of 489 aa containing two AP2 domains which indicated that it belonged to the AP2 subfamily of AP2/EREBP family. Comparison analysis showed that PeAP2 protein shared high homology with AP2 protein from other monocotyledon plants, especially with that of Brachypodium distachyon up to 74.85%. Real time PCR analysis showed that PeAP2 expressed all in root, stem, leaf, sheath and joint. The highest expressed level presented in leaf, followed by sheath and much lower in root, stem and joint. A 1 359 bp promoter sequence of PeAP2 gene was cloned by hiTAIL-PCR methods. The sequence analysis showed that it contained many cis-acting elements that response to light, hormone, and other factors.
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Cloning and Express Analysis of PeAP2 Gene and Its Promoter in Phyllostachys edulis

  • 1. International Centre for Bamboo and Rattan, Key Laboratory on the Science and Technology of Bamboo and Rattan, Beijing 100102
  • 2. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Abstract: APETALA2 (AP2) gene plays an important role in plant development. A full-length cDNA sequence of AP2 homologous gene was cloned from Phyllostachys edulis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods, and named as PeAP2. The PeAP2 is 1 750 bp and consists of a 5'-untranslated region (UTR) of 106 bp, a 3'-UTR of 174 bp and an opening reading frame of 1 470 bp. The sequence analysis showed that PeAP2 encoded a protein of 489 aa containing two AP2 domains which indicated that it belonged to the AP2 subfamily of AP2/EREBP family. Comparison analysis showed that PeAP2 protein shared high homology with AP2 protein from other monocotyledon plants, especially with that of Brachypodium distachyon up to 74.85%. Real time PCR analysis showed that PeAP2 expressed all in root, stem, leaf, sheath and joint. The highest expressed level presented in leaf, followed by sheath and much lower in root, stem and joint. A 1 359 bp promoter sequence of PeAP2 gene was cloned by hiTAIL-PCR methods. The sequence analysis showed that it contained many cis-acting elements that response to light, hormone, and other factors.

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